Plasmid pAO1 of Arthrobacter oxidans encodes 6-hydroxy-d-nicotine oxidase: cloning and expression of the gene in Escherichia coli

1986 ◽  
Vol 202 (1) ◽  
pp. 96-101 ◽  
Author(s):  
Roderich Brandsch ◽  
Waltraud Faller ◽  
Klaus Schneider
Keyword(s):  
Escherichia Coli ◽  
Cloning And Expression ◽  
Arthrobacter Oxidans

scholarly journals A novel chitosanase from Bacillus sp. Strain K17: Gene cloning and expression in Escherichia coli

2002 ◽  
Vol 2 (1) ◽  
pp. 227-228 ◽  
Author(s):  
R. Yatsunami ◽  
Y. Sakihama ◽  
M. Suzuki ◽  
T. Fukazawa ◽  
S. Shimizu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Cloning ◽  
Cloning And Expression ◽  
Gene Cloning And Expression ◽  
Bacillus Sp ◽  
Expression In Escherichia Coli

Cloning and expression of the Bacillus subtilis methyltransferase gene in Escherichia coli ada− cells

1989 ◽  
Vol 218 (2) ◽  
pp. 153-163 ◽  
Author(s):  
Ken-ichi Kodama ◽  
Yusaku Nakabeppu ◽  
Mutsuo Sekiguchi
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Cloning And Expression ◽  
Methyltransferase Gene

scholarly journals Molecular cloning and expression of the porcine S14R gene in Escherichia coli

2013 ◽  
Vol 12 (4) ◽  
pp. 4405-4412
Author(s):  
Y.J. Guo ◽  
G.Z. Liu ◽  
C.M. Wang ◽  
Y.Y. Wang ◽  
H.J. Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Cloning ◽  
Cloning And Expression

scholarly journals Escherichia coli as a genetic tool

1985 ◽  
Vol 95 (3) ◽  
pp. 611-618
Author(s):  
Naomi Datta
Keyword(s):  
Escherichia Coli ◽  
Academic Research ◽  
Genetically Engineered ◽  
Cloning And Expression ◽  
Biological Research ◽  
New Techniques ◽  
E Coli ◽  
Genetic Tool ◽  
The Past ◽  
Made In

SUMMARYThe study of Escherichia coli and its plasmids and bacteriophages has provided a vast body of genetical information, much of it relevant to the whole of biology. This was true even before the development of the new techniques, for cloning and analysing DNA, that have revolutionized biological research during the past decade. Thousands of millions of dollars are now invested in industrial uses of these techniques, which all depend on discoveries made in the course of academic research on E. coli. Much of the background of knowledge necessary for the cloning and expression of genetically engineered information, as well as the techniques themselves, came from work with this organism.

Cloning and expression of Xylella fastidiosa antigens in Escherichia coli and Erwinia stewartii

1989 ◽  
Vol 35 (4) ◽  
pp. 487-491 ◽  
Author(s):  
Paul H. Goodwin
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Genomic Dna ◽  
Xylella Fastidiosa ◽  
Gene Bank ◽  
Cloning And Expression ◽  
Western Blots ◽  
E Coli ◽  
Cosmid Vector ◽  
Erwinia Stewartii

Xylella fastidiosa DNA, partially digested with Sau3A, was ligated into the cosmid vector, pUCD615. Approximately 4500 ampicillin-resistant Escherichia coli colonies were obtained. The frequency of complementation of leucine auxotrophy in transfected E. coli indicated that the cosmid gene bank was representative of X. fastidiosa genomic DNA. Colonies were lysed directly onto nitrocellulose membranes using a thermo-inducible λ lysogen and screened for expression of X. fastidiosa antigens. Approximately 16.5% of a random sample of clones were found to express X. fastidiosa antigens as determined by Western blots. These proteins comigrated with proteins of X. fastidiosa and ranged in molecular weight from 10 000 to 160 000. Conjugation of several of the plasmids into Erwinia stewartii resulted in expression of the similar molecular weight cloned proteins with similar levels of expression as in E. coli.Key words: Xylella fastidiosa, Pierce's disease, immunological clone screening, thermo-inducible lysogeny.

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