024 Size-exclusion chromatography with simultaneous low-angle laser light scattering, ultraviolet and refractive index detection: absolute molecular mass determination with online determination of the specific refractive index increment

1992 ◽  
Vol 343 (1) ◽  
pp. 76-77 ◽  
Author(s):  
R�diger Kock ◽  
Helmut Greiling
Keyword(s):  
Refractive Index ◽  
Laser Light ◽  
Laser Light Scattering ◽  
Size Exclusion ◽  
Mass Determination ◽  
Refractive Index Increment ◽  
Exclusion Chromatography ◽  
Molecular Mass Determination ◽  
Refractive Index Detection

Fractional Separation and Estimation of Mark-Houwink-Sakurada Equation Parameters for Gluten

2012 ◽  
Vol 8 (1) ◽  
Author(s):  
Lei Zhao ◽  
Lin Li ◽  
Guoqin Liu ◽  
Ling Chen ◽  
Xiaoxi Li ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Liquid Chromatography ◽  
Light Scattering ◽  
Laser Light ◽  
Average Molecular Weight ◽  
Laser Light Scattering ◽  
Size Exclusion ◽  
Gluten Proteins ◽  
Exclusion Chromatography

Fractional separation of gluten using the fast protein liquid chromatography (FPLC) and a numerical method for determination of Mark-Houwink-Sakurada (MHS) equation were studied. With 1% SDS buffer as solvent, the gluten was disassembled into two components: SDS soluble (SDS-S-G) and SDS insoluble gluten proteins (SDS-IS-G). The average molecular weight (Mn, Mw and Mz) and intrinsic viscosity [η] were measured by the multi-angle laser light scattering (MALLS) in conjunction with a size-exclusion chromatography (SEC) and viscosimetry. MHS equations for SDS soluble gluten and SDS insoluble gluten proteins were established as: (SDS-S-G) [η]=1.7459×10-2Mv0.6933=1.7459×10-2qMHSMw0.6933=1.6677×10-2Mw0.6933 (SDS-IS-G) [η]=7.5682×10-3Mv0.7323=7.5682×10-3qMHSMw0.7323=7.2079×10-3Mw0.7323 where qMHS was the polydispersity correction factor.

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