Rheological and textural analysis as tools for investigation of drug-polymer and polymer–polymer interactions on the example of low-acyl gellan gum and mesalazine

Purpose. In the performed study, the rheological and textural parameters of gellan-based hydrogels were investigated and their dependence on three factors was taken into consideration: ( i) The presence of the model drug, ( ii) The presence and type of the ionic crosslinking agent, and ( iii) the composition of the polymer network. The objective was to compare two analytical methods, regarded as complementary, and define to what extent the obtained results correlate with each other. Methods. The hydrogels contained low-acyl gellan gum or its mixtures with hydroxyethyl cellulose or κ-carrageenan. CaCl2 and MgCl2 were used as gelling agents. Mesalazine was used as a model drug. The rheological analysis included oscillatory stress and frequency sweeping. The texture profile analysis was performed to calculate texture parameters. Results. Placebo gels without the addition of gelling agents had the weakest structure. The drug had the strongest ability to increase the stiffness of the polymer network. The weakest structure revealed the placebo samples without the addition of gelling agents. Texture analysis revealed no significant influence of the drug on the strength of the gels, while rheological measurements indicated clear differences. Conclusions. It can be concluded that in the case of some parameters methods correlate, that is, the effect related to gelling ions. However, the rheological analysis seems to be more precise and sensitive to some changes in the mechanical properties of the gels.

Bio-Based Hydrogel and Aerogel Composites Prepared by Combining Cellulose Solutions and Waterborne Polyurethane

Hydrogel composites can be prepared from cellulose-based materials and other gel materials, thus combining the advantages of both kinds of material. The aerogel, porous material formed after removing the water in the hydrogel, can maintain the network structure. Hydrogel and aerogel have high application potential. However, low mechanical strength and weight loss of cellulose hydrogel due to the water dehydration/absorption limit the feasibility of repeated use. In this study, cellulose hydrogels were prepared using microcrystalline cellulose (MC), carboxymethyl cellulose (CMC), and hydroxyethyl cellulose (HEC) as raw materials. Waterborne polyurethane (WPU) was added during the preparation process to form cellulose/WPU composite hydrogel and aerogel. The influence of the cellulose type and WPU addition ratio on the performance of hydrogel and aerogel were investigated. The results show that the introduction of WPU can help strengthen and stabilize the structure of cellulose hydrogel, reduce weight loss caused by water absorption and dehydration, and improve its reusability. The mixing of cellulose and WPU at a weight ratio of 90/10 is the best ratio to make the cellulose/WPU composite aerogel with the highest water swelling capacity and heat resistance.

Development of an Antifungal Device Based on Oriental Mustard Flour to Prevent Fungal Growth and Aflatoxin B1 Production in Almonds

The present study describes the manufacture of an antifungal device composed of oriental mustard flour and hydroxyethyl-cellulose (H-OMF) and evaluates its efficacity in inhibiting Aspergillus flavus growth and aflatoxin B1 (AFB1) production in almonds. Additionally, it compares the H-OMF with allyl isothiocyanate (AITC) and a freeze-dried extract of yellow mustard flour (YMF-E); such substances were previously described as antifungal. Minimum inhibitory concentration (MIC), Minimum fungicidal concentration (MFC), the H-OMF in vitro antifungal activity, and the residual fungal population, as well as the production of AFB1 in almonds were determined. AITC and YMF-E showed significant antifungal activity in vitro. Additionally, the in vitro activity of H-OMF avoided mycelial growth by applying 30 mg/L. Almonds treated with AITC (5.07, 10.13, and 20.26 mg/L) and H-OMF (2000 and 4000 mg/L) showed a reduction in the population of A. flavus and the production of AFB1 to values below the limit of detection. YMF-E showed effectiveness by in vitro methodologies (MIC and MFC) but did not show efficacy when applied in almonds. Our findings indicated that the hydroxyethyl-cellulose-based device containing oriental mustard flour might be utilised as a fumigant to increase the safety of almonds and could be extended to other cereals or dry fruits.

Degradation-Dependent Stress Relaxing Semi-Interpenetrating Networks of Hydroxyethyl Cellulose in Gelatin-PEG Hydrogel with Good Mechanical Stability and Reversibility

The mechanical milieu of the extracellular matrix (ECM) plays a key role in modulating the cellular responses. The native ECM exhibits viscoelasticity with stress relaxation behavior. Here, we reported the preparation of degradation-mediated stress relaxing semi-interpenetrating (semi-IPN) polymeric networks of hydroxyethyl cellulose in the crosslinked gelatin-polyethylene glycol (PEG) architecture, leveraging a newly developed synthesis protocol which successively includes one-pot gelation under physiological conditions, freeze-drying and a post-curing process. Fourier transform infrared (FTIR) confirmed the formation of the semi-IPN blend mixture. A surface morphology analysis revealed an open pore porous structure with a compact skin on the surface. The hydrogel showed a high water-absorption ability (720.00 ± 32.0%) indicating the ability of retaining a hydrophilic nature even after covalent crosslinking with functionalized PEG. Detailed mechanical properties such as tensile, compressive, cyclic compression and stress relaxation tests were conducted at different intervals over 28 days of hydrolytic degradation. Overall, the collective mechanical properties of the hydrogel resembled the mechanics of cartilage tissue. The rate of stress relaxation gradually increased with an increasing swelling ratio. Hydrolytic degradation led to a marked increase in the percentage dissipation energy and stress relaxation response, indicating the degradation-dependent viscoelasticity of the hydrogel. Strikingly, the hydrogel maintained the structural stability even after degrading two-thirds of its initial mass after a month-long hydrolytic degradation. This study demonstrates that this semi-IPN G-PEG-HEC hydrogel possesses bright prospects as a potential scaffolding material in tissue engineering.

Flow regimes during fluid-filled crack propagation in elastic media: Applications to volcanology and magma flow within dykes

Scaled analogue experiments were conducted to explore the effect of magma flow regimes, characterised by the Reynolds number (Re), on the transit of magma through the lithosphere via fractures. An elastic, transparent gelatine solid (the crust analogue) was injected by a fluid (magma analogue) to create a thin, vertical, and penny-shaped crack that is analogous to a magma-filled crack (dyke). A vertical laser sheet fluoresced passive-tracer particles suspended in the injected fluid, and particle image velocity (PIV) was used to map the location, magnitude, and direction of flow within the growing dyke from its inception to its surface rupture. Experiments were conducted using water, hydroxyethyl cellulose (HEC) or xanthan gum (XG) as the magma analogue. The results suggest that Re has significant impact on the direction of fluid flow within propagating dykes: Re > 0.1 (jet-flow) is characterised by a rapid central rising fluid jet and downflow at the dyke margin, whereas Re < 0.1 (creeping flow) is characterised by broadly uniform velocities across the dyke plane. Re may be underestimated by up to two orders of magnitude if tip velocity rather than internal fluid velocity is used. In nature, these different flow regimes would affect the petrological, geochemical, geophysical, and geodetic measurements of magma movement, key information upon which reconstructions of volcanic plumbing system architectures and their growth are based.

Nano-SiO2/hydroxyethyl cellulose nanocomposite used for 210 °C sedimentation control of petroleum drilling fluid

Cellulose derivatives are widely applied in the field of oil and gas exploration. However, this kind of natural polymers always shows poor temperature resistance due to their organic nature. To improve the temperature resistance of hydroxyethyl cellulose (HEC), inorganic nano-SiO2 was introduced onto HEC polymer chains through the silylation coupling technique. And Fourier transform infrared spectrum (FTIR), X-ray photoelectron spectrum (XPS), and thermogravimetic analysis (TGA) were used to analyze the nanocomposite. As a result, nano-SiO2 particle is chemically coupled onto hydroxyethyl cellulose molecule, and nano-SiO2/hydroxyethyl cellulose nanocomposite (RJ-HEC) shows excellent thermal stability comparing with HEC polymer. In experiment, thermal aging tests were utilized, and test results suggest that nano-SiO2/hydroxyethyl cellulose (RJ-HEC) nanocomposite can be utilized as thickening agent of water-based drilling fluid, which shows improved rheology stability at 210 °C and excellent salt (NaCl) tolerance.