047 Biospecific interaction analysis using surface plasmon resonance detection. Mapping of binding sites on IGF-II and kinetic analysis of the interaction of IGF-II with IGFBP-1 and the IGF-II receptor

1992 ◽  
Vol 343 (1) ◽  
pp. 100-101
Author(s):  
Robert Karlsson
Keyword(s):  
Surface Plasmon Resonance ◽  
Kinetic Analysis ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Sites ◽  
Interaction Analysis ◽  
Biospecific Interaction ◽  
Resonance Detection

scholarly journals Peptide–protein microarrays and surface plasmon resonance detection: Biosensors for versatile biomolecular interaction analysis

2010 ◽  
Vol 26 (4) ◽  
pp. 1554-1559 ◽  
Author(s):  
Marie-Bernadette Villiers ◽  
Sandra Cortès ◽  
Carine Brakha ◽  
Jean-Pierre Lavergne ◽  
Christophe A. Marquette ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Interaction Analysis ◽  
Protein Microarrays ◽  
Biomolecular Interaction ◽  
Biomolecular Interaction Analysis ◽  
Resonance Detection

scholarly journals Surface Plasmon Resonance for Detection of Genetically Modified Organisms in the Food Supply

2006 ◽  
Vol 89 (3) ◽  
pp. 893-897 ◽  
Author(s):  
Roberto Gambari ◽  
Giordana Feriotto
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Genetically Modified Organisms ◽  
Interaction Analysis ◽  
Genetically Modified ◽  
Research Field ◽  
Pcr Products ◽  
Value Determination ◽  
Biospecific Interaction

Abstract A review is presented demonstrating that biospecific interaction analysis, using surface plasmon resonance (SPR) and biosensor technologies is a simple, rapid, and automatable approach to detect genetically modified organisms (GMOs). Using SPR, we were able to monitor in real-time the hybridization between oligonucleotide or polymerase chain reaction (PCR)-generated probes and target single-stranded PCR products obtained by using as substrates DNA isolated from normal or transgenic soybean and maize. This procedure allows a one-step, nonradioactive detection of GMOs. PCR-generated probes are far more efficient in detecting GMOs than are oligodeoxyribonucleotide probes. This is expected to be a very important parameter, because information on low percentage of GMOs is of great value. Determination of the ability of SPR-based analysis to quantify GMOs should be considered a major research field for future studies, especially for the analyses of food supplies.

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