Plant genetic engineering for crop improvement

1995 ◽  
Vol 11 (4) ◽  
pp. 449-460 ◽  
Author(s):  
G. Kahl ◽  
P. Winter
Author(s):  
B. D. Harrison

SynopsisSome of the most successful early applications of genetic engineering in crop improvement have been in the production of virus-resistant plants. This has been achieved not by the transfer of naturally occurring resistance genes from one plant species or variety to another but by transformation with novel resistance genes based on nucleotide sequences derived from the viruses themselves or from virus-associated nucleic acids. Transformation of plants with a DNA copy of the particle protein gene of viruses that have positive-sense single-stranded RNA genomes typically confers resistance to infection with the homologous and closely related viruses. Transformation with a gene that is transcribed to produce a benign viral satellite RNA can confer virus-specific tolerance of infection. In addition, recent work with viral poly-merase gene-related sequences offers much promise, and research is active on other strategies such as the use of virus-specific ribozymes.Already the field trialling of plants incorporating transgenic virus resistance has begun, with encouraging results, and effects on virus spread are being studied. Deployment strategies for the resistant plants must now be devised and the conjectural hazards of growing them assessed. Genetically engineered virus resistance promises to make a major contribution to the control of plant virus diseases by non-chemical methods.


1998 ◽  
pp. 339-345
Author(s):  
Kathleen D’Halluin ◽  
Johan Botterman

Plant Biology ◽  
2021 ◽  
pp. 192-197
Author(s):  
Andrew Lack ◽  
David Evans

Author(s):  
Javid Ahmad Parray ◽  
Mohammad Yaseen Mir ◽  
Nowsheen Shameem

2019 ◽  
Vol 116 (28) ◽  
pp. 13816-13824 ◽  
Author(s):  
Kwang Ho Kim ◽  
Aymerick Eudes ◽  
Keunhong Jeong ◽  
Chang Geun Yoo ◽  
Chang Soo Kim ◽  
...  

Despite the enormous potential shown by recent biorefineries, the current bioeconomy still encounters multifaceted challenges. To develop a sustainable biorefinery in the future, multidisciplinary research will be essential to tackle technical difficulties. Herein, we leveraged a known plant genetic engineering approach that results in aldehyde-rich lignin via down-regulation of cinnamyl alcohol dehydrogenase (CAD) and disruption of monolignol biosynthesis. We also report on renewable deep eutectic solvents (DESs) synthesized from phenolic aldehydes that can be obtained fromCADmutant biomass. The transgenicArabidopsis thaliana CADmutant was pretreated with the DESs and showed a twofold increase in the yield of fermentable sugars compared with wild type (WT) upon enzymatic saccharification. Integrated use of low-recalcitrance engineered biomass, characterized by its aldehyde-type lignin subunits, in combination with a DES-based pretreatment, was found to be an effective approach for producing a high yield of sugars typically used for cellulosic biofuels and biobased chemicals. This study demonstrates that integration of renewable DES with plant genetic engineering is a promising strategy in developing a closed-loop process.


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