Involvement of single-strand breaks in complex formation between single-stranded DNA and nucleoids of Bacillus subtilis

1984 ◽  
Vol 193 (3) ◽  
pp. 500-506
Author(s):  
Jan van Randen ◽  
Koos Wiersma ◽  
Gerard Venema
Keyword(s):  
Bacillus Subtilis ◽  
Complex Formation ◽  
Single Strand ◽  
Single Stranded Dna ◽  
Strand Breaks ◽  
Single Strand Breaks

Experimentelle Hinweise auf intramolekulare Energieleitung in der hybriden DNA von Bacillus subtilis nach Bestrahlung mit langwelligem UV-Licht / Experimental Evidence for Intramolecular Energy Transfer in Hybrid DNA of B. subtilis after Irradiation with Long Wavelength UV

1972 ◽  
Vol 27 (6) ◽  
pp. 708-713 ◽  
Author(s):  
W. Köhnlein ◽  
F. Mönkehaus
Keyword(s):  
Bacillus Subtilis ◽  
Energy Transfer ◽  
Experimental Evidence ◽  
Analytical Ultracentrifugation ◽  
Single Strand ◽  
Intramolecular Energy Transfer ◽  
Strand Breaks ◽  
Long Wavelength ◽  
Single Strand Breaks ◽  
Hybrid Dna

Investigations on hybrid DNA of B. subtilis after irradiation with long wavelength UV (313 nm) indicate that single strand breaks are produced in the T-strand. These lesions are not observed in normal DNA. Since the action of diffusible photoproducts could be excluded the occurrence of single strand breaks in the T-strand may be due to intramolecular energy transfer. Measuring the breakage rates for the T- and B-strand by employing analytical ultracentrifugation it was found that up to 5% of the energy absorbed in the B-strand can be transferred to the T-strand and causes there single strand breaks. The breakage rates per erg-mm- 2 for single strand breaks after long wavelength UV are determined to be aT = 0.5 · 10-4 and aB = 10 · 10-4 breaks/106 Dalton for the T- and B-strand of hybrid DNA respectively.

scholarly journals Artificial and Solar UV Radiation Induces Strand Breaks and Cyclobutane Pyrimidine Dimers in Bacillus subtilis Spore DNA

2000 ◽  
Vol 66 (1) ◽  
pp. 199-205 ◽  
Author(s):  
Tony A. Slieman ◽  
Wayne L. Nicholson
Keyword(s):  
Bacillus Subtilis ◽  
Uv Irradiation ◽  
Double Strand Breaks ◽  
Single Strand ◽  
Complex Spectrum ◽  
Cyclobutane Pyrimidine Dimers ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Pyrimidine Dimers ◽  
Solar Uv

ABSTRACT The loss of stratospheric ozone and the accompanying increase in solar UV flux have led to concerns regarding decreases in global microbial productivity. Central to understanding this process is determining the types and amounts of DNA damage in microbes caused by solar UV irradiation. While UV irradiation of dormant Bacillus subtilis endospores results mainly in formation of the “spore photoproduct” 5-thyminyl-5,6-dihydrothymine, genetic evidence indicates that an additional DNA photoproduct(s) may be formed in spores exposed to solar UV-B and UV-A radiation (Y. Xue and W. L. Nicholson, Appl. Environ. Microbiol. 62:2221–2227, 1996). We examined the occurrence of double-strand breaks, single-strand breaks, cyclobutane pyrimidine dimers, and apurinic-apyrimidinic sites in spore DNA under several UV irradiation conditions by using enzymatic probes and neutral or alkaline agarose gel electrophoresis. DNA from spores irradiated with artificial 254-nm UV-C radiation accumulated single-strand breaks, double-strand breaks, and cyclobutane pyrimidine dimers, while DNA from spores exposed to artificial UV-B radiation (wavelengths, 290 to 310 nm) accumulated only cyclobutane pyrimidine dimers. DNA from spores exposed to full-spectrum sunlight (UV-B and UV-A radiation) accumulated single-strand breaks, double-strand breaks, and cyclobutane pyrimidine dimers, whereas DNA from spores exposed to sunlight from which the UV-B component had been removed with a filter (“UV-A sunlight”) accumulated only single-strand breaks and double-strand breaks. Apurinic-apyrimidinic sites were not detected in spore DNA under any of the irradiation conditions used. Our data indicate that there is a complex spectrum of UV photoproducts in DNA of bacterial spores exposed to solar UV irradiation in the environment.

scholarly journals Single Strand Breaks Induced in DNA of Bacillus subtilis Cells by Mild Heating

1977 ◽  
Vol 43 (6) ◽  
pp. 779-779
Author(s):  
Aritsune UCHIDA ◽  
Yoshihiko SAKO ◽  
Hajime KADOTA
Keyword(s):  
Bacillus Subtilis ◽  
Single Strand ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
I Cells

scholarly journals Damaged DNA in lymphocytes of aplastic anemia

Blood ◽  
1975 ◽  
Vol 46 (5) ◽  
pp. 735-742 ◽  
Author(s):  
Y Hashimoto ◽  
F Takaku ◽  
K Kosaka
Keyword(s):  
Aplastic Anemia ◽  
Sucrose Gradient ◽  
Gradient Centrifugation ◽  
Single Strand ◽  
Single Stranded Dna ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Circulating Lymphocytes ◽  
The Difference ◽  
Average Size

Abstract The size of single-stranded DNA in lymphocytes in GO stage from 22 patients with acquired primary and secondary aplastic anemia was estimated by alkaline sucrose gradient centrifugation. The average size was 9.3 (+/-0.3) times 10(7) daltons. The lymphocytes of patients contained significantly more single-strand breaks in DNA, compared to those of normal persons. The difference in size of single-stranded DNA which had been present in nontransformed lymphocytes could also be observed in transformed lymphocytes. Some characteristic differences could be observed in the sedimentation patterns of single-stranded DNA in the lymphocytes of patients with aplastic anemia and those of normal persons. The single-strand breaks in DNA suggested that the repair processes were disturbed in the DNA molecules of circulating lymphocytes from patients with acquired primary and secondary aplastic anemia.

scholarly journals Damaged DNA in lymphocytes of aplastic anemia

Blood ◽  
1975 ◽  
Vol 46 (5) ◽  
pp. 735-742
Author(s):  
Y Hashimoto ◽  
F Takaku ◽  
K Kosaka
Keyword(s):  
Aplastic Anemia ◽  
Sucrose Gradient ◽  
Gradient Centrifugation ◽  
Single Strand ◽  
Single Stranded Dna ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Circulating Lymphocytes ◽  
The Difference ◽  
Average Size

The size of single-stranded DNA in lymphocytes in GO stage from 22 patients with acquired primary and secondary aplastic anemia was estimated by alkaline sucrose gradient centrifugation. The average size was 9.3 (+/-0.3) times 10(7) daltons. The lymphocytes of patients contained significantly more single-strand breaks in DNA, compared to those of normal persons. The difference in size of single-stranded DNA which had been present in nontransformed lymphocytes could also be observed in transformed lymphocytes. Some characteristic differences could be observed in the sedimentation patterns of single-stranded DNA in the lymphocytes of patients with aplastic anemia and those of normal persons. The single-strand breaks in DNA suggested that the repair processes were disturbed in the DNA molecules of circulating lymphocytes from patients with acquired primary and secondary aplastic anemia.

DNA Single Strand Breaks Induced by Low Levels of Occupational Exposure to Styrene: The Gap between Standards and Reality

2002 ◽  
Vol 21 (1) ◽  
pp. 5 ◽  
Author(s):  
Magdy Y. Shamy ◽  
Hazem H. Osman ◽  
Kamal M. Kandeel ◽  
Nehad M. Abdel-Moneim ◽  
Khalid F. El Said
Keyword(s):  
Occupational Exposure ◽  
Single Strand ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Low Levels
Sign in / Sign up

Export Citation Format

Share Document