Simultaneous inhibition of translocation of photosynthate and of the floral stimulus by localized low-temperature treatment in the short-day plant Pharbitis nil

Planta ◽  
1979 ◽  
Vol 144 (2) ◽  
pp. 201-204 ◽  
Author(s):  
David L. Kavon ◽  
Jan A. D. Zeevaart

2006 ◽  
Vol 131 (4) ◽  
pp. 551-563 ◽  
Author(s):  
Carole L. Bassett ◽  
Michael E. Wisniewski ◽  
Timothy S. Artlip ◽  
John L. Norelli ◽  
Jenny Renaut ◽  
...  

In response to environmental cues plants undergo changes in gene expression that result in the up- or down-regulation of specific genes. To identify genes in peach [Prunus persica (L.) Batsch.] trees whose transcript levels are specifically affected by low temperature (LT) or short day photoperiod (SD), we have created suppression subtractive hybridization (SSH) libraries from bark tissues sampled from trees kept at 5 °C and 25 °C under short day (SD) photoperiod or exposed to a night break (NB) interruption during the dark period of the SD cycle to simulate a long day (LD) photoperiod. Sequences expressed in forward and reverse subtractions using various subtracted combinations of temperature and photoperiod treatments were cloned, sequenced, and identified by BLAST and ClustalW analysis. Low temperature treatment resulted in the up-regulation of a number of cold-responsive and stress-related genes and suppression of genes involved in “housekeeping” functions (e.g., cell division and photosynthesis). Some stress-related genes not observed to be up-regulated under LT were increased in response to SD photoperiod treatments. Comparison of the patterns of expression as a consequence of different temperature and photoperiod treatments allowed us to determine the qualitative contribution of each treatment to the regulation of specific genes.



1982 ◽  
Vol 23 (3) ◽  
pp. 473-477 ◽  
Author(s):  
Masateru Shinozaki ◽  
Masayuki Hikichi ◽  
Kazuichi Yoshida ◽  
Kazuo Watanabe ◽  
Atsushi Takimoto


1972 ◽  
Vol 50 (12) ◽  
pp. 2683-2685 ◽  
Author(s):  
Richard P. Pharis ◽  
William Morf

Short-day (SD) and low temperature (5 °C) treatments were tested separately to ascertain their contribution to the anthesis-like development of gibberellin-induced staminate and ovulate strobili of western red cedar (Thuja plicata Donn.). Low temperature, when given only for 8 weeks under long day (LD) where the daily sequence of photoperiod was kept continuously at 16 h resulted in expansion of 90% and 77% of the staminate and ovulate strobili respectively compared to 96% and 79% respectively where cold was given only during 8 weeks of SD in the sequence LD → SD → LD. Conversely, 8 weeks of SD given at warm temperature (19 °C) in the sequence of LD → SD → LD resulted in expansion of 9% and 1% of the staminate and ovulate strobili respectively. Thus, strobili of western red cedar can be said to have a cold requirement for normal anthesis-like expansion. In only a very small percentage of the strobili can SD (at least 8 weeks of SD) substitute for low temperature, and the low temperature treatment is effective regardless of photoperiod.







1968 ◽  
Vol 48 (3) ◽  
pp. 597-609
Author(s):  
R. MORRIS ◽  
J. M. BULL

1. An investigation has been made of the factors which cause sodium loss from ammocoetes when they are immersed in de-ionized water at 1° and 10° C. 2. Sodium influx ceases when animals are first immersed in de-ionized water, but can recommence when the animal loses sufficient sodium to the environment. The concentration of sodium required for influx to take place decreases with succeeding periods of immersion in de-ionized water at 10° C. and reaches minimum equilibrium concentrations as low as 0.005 mM-Na/l. 3. Low temperature inhibits sodium influx and thus promotes net loss of sodium to de-ionized water. 4. Low temperature also decreases the initial loss of sodium to de-ionized water and probably lowers the permeability of the external surfaces of the animal to ions. This effect is small compared with the inhibition of ion uptake so that the combined result is to increase the net loss of sodium from the animal. 5. Since animals lose calcium to de-ionized water and show a decreased rate of sodium loss when calcium salts are added, it is believed that the high rates of sodium loss in de-ionized water are attributable to the effect of calcium on permeability. 6. Lack of calcium may also explain why animals which have been depleted of sodium by low-temperature treatment take up sodium much faster at higher temperatures from dilute Ringer solutions than from pure sodium chloride solutions. 7. When animals lose ions to de-ionized water at low temperature, sodium and chloride are lost from the extracellular space, whilst the muscle cells lose potassium. These ions are recovered into the extracellular space when animals are allowed to take up ions at 10° C. from diluted Ringer solution later.



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