Liquid nitrogen storage of yeast cultures. II. Stability of characteristics of stored strains

1983 ◽  
Vol 49 (6) ◽  
pp. 571-578 ◽  
Author(s):  
Anna Kockov�-Kratochv�lov� ◽  
Z. Hub�lek
Keyword(s):  
Liquid Nitrogen ◽  
Nitrogen Storage ◽  
Yeast Cultures

Liquid Nitrogen Storage of Yeast Cultures Compared to More Traditional Storage Methods

1981 ◽  
Vol 39 (1) ◽  
pp. 19-24 ◽  
Author(s):  
I. Russell ◽  
G. G. Stewart
Keyword(s):  
Liquid Nitrogen ◽  
Nitrogen Storage ◽  
Yeast Cultures ◽  
Storage Methods

Liquid nitrogen storage of yeast cultures I. Survival, and literature review of the preservation of fungi at ultralow temperatures

1978 ◽  
Vol 44 (2) ◽  
pp. 229-241 ◽  
Author(s):  
Z. Hub�lek ◽  
Anna Kockov�-Kratochv�lov�
Keyword(s):  
Literature Review ◽  
Liquid Nitrogen ◽  
Nitrogen Storage ◽  
Yeast Cultures ◽  
Ultralow Temperatures

What Factors Affect The Presence of Microorganisms In Cryotanks? - A Culture-Independent Approach To Assess Potential Microbial Colonization of Liquid Nitrogen Storage Tanks

Cryobiology ◽  
2021 ◽  
Vol 103 ◽  
pp. 161
Author(s):  
Felizitas Bajerski ◽  
Antje Bürger ◽  
Birgit Glasmacher ◽  
E.R. Joachim Keller ◽  
Karin Müller ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Microbial Colonization ◽  
Nitrogen Storage ◽  
Storage Tanks ◽  
Culture Independent

Isber And Sfc Partner To Produce The Liquid Nitrogen Storage Best Practices

Cryobiology ◽  
2019 ◽  
Vol 91 ◽  
pp. 182
Author(s):  
David Lewandowski
Keyword(s):  
Best Practices ◽  
Liquid Nitrogen ◽  
Nitrogen Storage

Liquid nitrogen storage of Anaplasma marginale complement-fixation antigen by a multiple small aliquot technique

1978 ◽  
Vol 25 (3) ◽  
pp. 401-402 ◽  
Author(s):  
R. Parker ◽  
M.L. Parker ◽  
A.J. Wilson
Keyword(s):  
Liquid Nitrogen ◽  
Complement Fixation ◽  
Anaplasma Marginale ◽  
Nitrogen Storage ◽  
Small Aliquot

Liquid Nitrogen Storage of Fungi Sealed In A Polyester Film

Mycologia ◽  
1968 ◽  
Vol 60 (3) ◽  
pp. 591-954 ◽  
Author(s):  
John Tuite
Keyword(s):  
Liquid Nitrogen ◽  
Nitrogen Storage ◽  
Polyester Film

A Study on the Weld Part Fracture Toughness of Austenite Type Stainless Steel for Cryogenic Liquid Nitrogen Storage Tank

2011 ◽  
Vol 35 (6) ◽  
pp. 802-808
Author(s):  
Young-Deuk Kim ◽  
Dong-Jun Choi ◽  
Hyung-Wook Park ◽  
Jong-Rae Cho ◽  
Won-Byoung Bae
Keyword(s):  
Fracture Toughness ◽  
Stainless Steel ◽  
Liquid Nitrogen ◽  
Storage Tank ◽  
Cryogenic Liquid ◽  
Nitrogen Storage ◽  
Type Stainless Steel

scholarly journals The Impact of Freezing Temperature and Pretreatment Protocol on Solid Phase HR-MAS-MRS Metabolomes of Colorectal Cancer Tissue

2021 ◽  
Author(s):  
Hui Liu ◽  
Yu Wang ◽  
Yue-qiang Han ◽  
Guang-yu Yang ◽  
Lu Wang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Liquid Nitrogen ◽  
Solid Phase ◽  
Phospholipid Metabolism ◽  
The Other ◽  
Cancer Tissue ◽  
Nitrogen Storage ◽  
Tissue Samples ◽  
Colorectal Cancer Tissue ◽  
Choline Phospholipid

Abstract Background: To explore the best pretreatment method of colorectal cancer tissue samples for metabolomics research based on solid-phase nuclear magnetic resonance. Method: Taking mucosal tissues of colorectal cancer and divide it into 5 groups of 0.2cm*0.2cm*0.2cm. Pretreatment was performed as follows: I. Liquid nitrogen storage; II. Transfer to the -80℃ refrigerator after storing in liquid nitrogen for 10 minutes; III. Transfer to the -80℃ refrigerator after storing in liquid nitrogen for 20 minutes; IV. Transfer to the -80℃ refrigerator after storing in liquid nitrogen for 30 minutes; V. -80℃ refrigerator storage. The interval between tumor sample separation to pretreatment is less than 30 minutes. The tissue sample testing process is carried out on Bruker AVII-600 Spectrometer equipped with a high-resolution probe having a 1H/13C magical angle rotation. The tissue samples were put into the NMR which run at a speed of 5000Hz for 10 minutes. NMR signals were collected and analyzed by Fourier transform, partial least squares discrimination analysis (PLS-DA). Corresponding metabolites and metabolic pathways were found in Human Metabolome Database (HMDB) according to the ppms with variable importance of projection (VIP) ＞1. Results: The content of pelargonic acid, stearic acid, D-Ribose, heptadecanoic acid, pyruvic acid, succinate, sarcosine, glycine, creatine, and L-lactate in liquid nitrogen storage group were significantly lower than the other groups (P<0.05), the content of glycerophosphocholine in liquid nitrogen storage group was lower than the other groups (P=0.055). Pyruvic, succinate and L-lactate are participating in glucose metabolism. Glycerophosphocholine, sarcosine, glycine and creatine are participating in choline phospholipid metabolism. This indicated that the glucose and choline phospholipid metabolism levels of the liquid nitrogen group were significantly lower than those of the other 4 groups. Conclusion: Liquid nitrogen storage can slow down the glucose and choline phospholipid metabolism process of colorectal cancer tissue samples in vitro; liquid nitrogen can preserve tissue sample’s metabolic state in the body. It is therefore the better way to store tissue sample than the other methods. clinical trial registry website: http://www.chictr.org.cn/index.aspx. Trial number: ChiCTR1900024640

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