Structural alterations in a transcribed region of the T type cytoplasmic male sterile maize mitochondrial genome

1986 ◽  
Vol 10 (10) ◽  
pp. 777-783 ◽  
Author(s):  
A. G. Abbott ◽  
C. M. R. Fauron
Keyword(s):  
Mitochondrial Genome ◽  
Male Sterile ◽  
Structural Alterations ◽  
Cytoplasmic Male Sterile

scholarly journals Unusual mitochondrial genome organization in cytoplasmic male sterile common bean and the nature of cytoplasmic reversion to fertility.

Genetics ◽  
1993 ◽  
Vol 135 (3) ◽  
pp. 869-879 ◽  
Author(s):  
H Janska ◽  
S A Mackenzie
Keyword(s):  
Mitochondrial Genome ◽  
Common Bean ◽  
Physical Map ◽  
Fertility Restoration ◽  
Nuclear Gene ◽  
Male Sterile ◽  
Unique Region ◽  
Cytoplasmic Male Sterile ◽  
Molecular Events ◽  
Fertile Revertant

Abstract Spontaneous reversion to pollen fertility and fertility restoration by the nuclear gene Fr in cytoplasmic male sterile common bean (Phaseolus vulgaris L.) are associated with the loss of a large portion of the mitochondrial genome. To understand better the molecular events responsible for this DNA loss, we have constructed a physical map of the mitochondrial genome of a stable fertile revertant line, WPR-3, and the cytoplasmic male sterile line (CMS-Sprite) from which it was derived. This involved a cosmid clone walking strategy with comparative DNA gel blot hybridizations. Mapping data suggested that the simplest model for the structure of the CMS-Sprite genome consists of three autonomous chromosomes differing only in short, unique regions. The unique region contained on one of these chromosomes is the male sterility-associated 3-kb sequence designated pvs. Based on genomic environments surrounding repeated sequences, we predict that chromosomes can undergo intra- and intermolecular recombination. The mitochondrial genome of the revertant line appeared to contain only two of the three chromosomes; the region containing the pvs sequence was absent. Therefore, the process of spontaneous cytoplasmic reversion to fertility likely involves the disappearance of an entire mitochondrial chromosome. This model is supported by the fact that we detected no evidence of recombination, excision or deletion events within the revertant genome that could account for the loss of a large segment of mitochondrial DNA.

scholarly journals Characterisation and expression of the mitochondrial genome of a new type of cytoplasmic male-sterile sunflower

1994 ◽  
Vol 26 (6) ◽  
pp. 1819-1831 ◽  
Author(s):  
Mariana Spassova ◽  
Fran�oise Moneger ◽  
Christopher J. Leaver ◽  
Peter Petrov ◽  
Atanas Atanassov ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
New Type

Mitochondrial genome organization of the maize cytoplasmic male sterile type T

1989 ◽  
Vol 216 (2-3) ◽  
pp. 395-401 ◽  
Author(s):  
Christiane Fauron ◽  
Marie Havlik ◽  
David Lonsdale ◽  
Lindy Nichols
Keyword(s):  
Mitochondrial Genome ◽  
Genome Organization ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

scholarly journals Characterization of the mitochondrial genome of the MAX1 type of cytoplasmic male-sterile sunflower

2019 ◽  
Vol 19 (S1) ◽  
Author(s):  
Maksim S. Makarenko ◽  
Alexander V. Usatov ◽  
Tatiana V. Tatarinova ◽  
Kirill V. Azarin ◽  
Maria D. Logacheva ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

scholarly journals Disruption of the mitochondrial gene orf352 partially restores pollen development in cytoplasmic male sterile rice

2021 ◽  
Author(s):  
Shiho Omukai ◽  
Shin-ich Arimura ◽  
Kinya Toriyama ◽  
Tomohiko Kazama
Keyword(s):  
Amino Acids ◽  
Mitochondrial Genome ◽  
Pollen Development ◽  
Mitochondrial Gene ◽  
Pollen Viability ◽  
Target Site ◽  
Transcription Activator ◽  
Male Sterile ◽  
Gene Encoding ◽  
Cytoplasmic Male Sterile

AbstractPlant mitochondrial genomes sometimes carry cytoplasmic male sterility (CMS)-associated genes. These genes have been harnessed in agriculture to produce high-yielding F1 hybrid seeds in various crops. The gene orf352 was reported to be an RT102-type CMS gene in rice (Oryza sativa), although a causal demonstration of its role in CMS is lacking. Here, we employed mitochondrion-targeted transcription activator-like effector nucleases (mitoTALENs), to knock out orf352 from the mitochondrial genome in the cytoplasmic male sterile rice RT102A. We isolated 18 independent transformation events in RT102A that resulted in genome editing of orf352, including its complete removal from the mitochondrial genome in several plants. Sequence analysis around the mitoTALEN target sites revealed the repair of their induced double-strand breaks via homologous recombination. Near the 5ʹ target site, repair involved sequences identical to orf284, while repair of the 3ʹ target site yielded various new sequences that generated new chimeric genes consisting orf352 fragments. Plants with a new mitochondrial gene encoding amino acids 179 to 352 of ORF352 exhibited the same shrunken pollen grain phenotype as RT102A, whereas plants either lacking orf352 or harboring a new gene encoding amino acids 211 to 352 of ORF352 showed partial rescue of pollen viability and germination, although they failed to set seed. These results demonstrated that disruption of orf352 partially restored pollen development, indicating that amino acids 179 to 210 from ORF352 may contribute to the establishment of pollen abortion.

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