Selection of symbiotically energy efficient strains of Rhizobium japonicum by their ability to induce a H2-uptake hydrogenase in the free-living state

1981 ◽  
Vol 128 (3) ◽  
pp. 275-279 ◽  
Author(s):  
T. Ruiz-Argüeso ◽  
E. Cabrera ◽  
Mirta Barate de Bertalmio
1980 ◽  
Vol 26 (3) ◽  
pp. 338-342 ◽  
Author(s):  
Laura de Vasconcelos ◽  
Llonas Miller ◽  
Carlos A. Neyra

This work investigated the usefulness of chlorate resistance as a method for the selection of nitrate reductase negative (NR−) strains from Rhizobium japonicum (61A76) and evaluated the symbiotic characteristics of these strains. Chlorate resistant strains were selected from populations seeded on CS 7 agar containing 10 or 20 mM KClO3 and incubated in 2% air – 98% N2–CO2 (95:5).Over 200 resistant strains were isolated, 58% of which lacked the dissimilatory nitrate reductase. In 12 selected isolates, some strains had also lost the assimilatory nitrate reductase, but all retained hydrogenase activity.Chlorate resistant strains inoculated to soybean seedlings were equal to or better than the parent strain in terms of nodule mass and acetylene reduction. Those strains lacking both assimilatory and dissimilatory nitrate reductase showed the best symbiotic characteristics, suggesting that chlorate resistance in R. japonicum could be a useful method for the selection of strains with superior nitrogen-fixing characteristics.


2014 ◽  
Vol 134 (12) ◽  
pp. 1022-1030 ◽  
Author(s):  
Tomoyuki Ogawa ◽  
Kazumasa Kumazawa ◽  
Noriaki Sugita ◽  
Yoichi Imamura ◽  
Shingo Minobe ◽  
...  

Marine Drugs ◽  
2019 ◽  
Vol 17 (10) ◽  
pp. 561 ◽  
Author(s):  
Anna Fidor ◽  
Robert Konkel ◽  
Hanna Mazur-Marzec

Cyanobacteria of the genus Nostoc are widespread in all kinds of habitats. They occur in a free-living state or in association with other organisms. Members of this genus belong to prolific producers of bioactive metabolites, some of which have been recognized as potential therapeutic agents. Of these, peptides and peptide-like structures show the most promising properties and are of a particular interest for both research laboratories and pharmaceutical companies. Nostoc is a sole source of some lead compounds such as cytotoxic cryptophycins, antiviral cyanovirin-N, or the antitoxic nostocyclopeptides. Nostoc also produces the same bioactive peptides as other cyanobacterial genera, but they frequently have some unique modifications in the structure. This includes hepatotoxic microcystins and potent proteases inhibitors such as cyanopeptolins, anabaenopeptins, and microginins. In this review, we described the most studied peptides produced by Nostoc, focusing especially on the structure, the activity, and a potential application of the compounds.


Author(s):  
R. P. Johnston ◽  
P. Ortiz

Details of the NASA sponsored General Electric Energy Efficient Engine (E3) technology program are presented along with a description of the engine, cycle and aircraft system benefits. Opportunities for further performance improvement beyond E3 are examined. Studies leading to the selection of the E3 cycle and configuration are summarized. The advanced technology features, cycle and component performance levels are also presented. An evaluation of the benefits of the fully developed Flight Propulsion System (FPS) is made relative to the NASA program goals by comparing the FPS with the CF6-50C where both are installed in advanced subsonic transport aircraft. Results indicate that a mission fuel saving from 15 to 23 percent is possible depending on mission length.


1964 ◽  
Vol 21 (3) ◽  
pp. 429-463 ◽  
Author(s):  
Elliott Robbins ◽  
Nicholas K. Gonatas

With a technique of preselecting the mitotic cell in the living state for subsequent electron microscopy, it has been possible to examine the ultrastructure of the various stages of mitosis with greater precision than has been reported previously. The early dissolution of the nuclear envelope has been found to be preceded by a marked undulation of this structure within the nuclear "hof." This undulation appears to be intimately related to the spindle-forming activity of the centriole at this time. Marked pericentriolar osmiophilia and extensive arrays of vesicles are also prominent at this stage, the former continuing into anaphase. Progression of the cell through prophase is accompanied by a disappearance of these vesicles. A complex that first makes its appearance in prophase but becomes most prominent in metaphase is a partially membrane-bounded cluster of dense osmiophilic bodies. These clusters which have a circumferential distribution in the mitotic cell are shown to be derived from multivesicular bodies and are acid phosphatase-positive. The precise selection of cells during the various stages of anaphase has made it possible to follow chronologically the morphological features of the initiation of nuclear membrane reformation. The nuclear membrane appears to be derived from polar aggregates of endoplasmic reticulum, and the process begins less than 2 minutes after the onset of karyokinesis. While formation of the nuclear envelope is initiated on the polar aspects of the chromatin mass, envelope elements appear on the equatorial aspect long before the polar elements fuse. Apparently interfering with this fusion are continuous spindle tubules which traverse the chromatin mass in striking density at characteristic points. Several cortical changes, also most pronounced in anaphase, have been described, as has the kinetochore which is seen to good advantage only in this stage. The Golgi complex has been found to disappear both morphologically and histochemically during mitosis and to reappear rapidly in telophase. Evidence is presented which implicates the continuous spindle tubules in certain phases of chromosome movement.


Author(s):  
Diarmuid Corcoran ◽  
Loghman Andimeh ◽  
Andreas Ermedahl ◽  
Per Kreuger ◽  
Christian Schulte

Sign in / Sign up

Export Citation Format

Share Document