Temperature-sensitive DNA repair mutations in the cellular slime mould Dictyostelium discoideum

1981 ◽  
Vol 3 (3) ◽  
pp. 167-171 ◽  
Author(s):  
Dennis L. Welker ◽  
Keith L. Williams
Keyword(s):  
Dna Repair ◽  
Dictyostelium Discoideum ◽  
Temperature Sensitive ◽  
Cellular Slime Mould ◽  
Slime Mould ◽  
Cellular Slime

scholarly journals A GENETIC STUDY OF AGGREGATION IN THE CELLULAR SLIME MOULD DICTYOSTELIUM DISCOIDEUM USING COMPLEMENTATION ANALYSIS

Genetics ◽  
1976 ◽  
Vol 82 (2) ◽  
pp. 287-307
Author(s):  
Keith L Williams ◽  
Peter C Newell
Keyword(s):  
Dictyostelium Discoideum ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Resistance Mutations ◽  
Partial Dominance ◽  
Parasexual Cycle ◽  
Cellular Slime Mould ◽  
Slime Mould ◽  
Cellular Slime ◽  
Interallelic Complementation

ABSTRACT A series of aggregation-deficient (aggregateless) mutants were isolated in genetically marked haploid strains of the cellular slime mould Dictyostelium discoideum. Diploids were produced from pairs of such haploid mutants by a fusion system based on this organism's parasexual cycle. The diploids were isolated from the haploids by using complementation of non-allelic growth-temperature-sensitive mutations and selection at the restrictive temperature. Complementation between the aggregateless mutations was then assessed in 419 diploids so formed. The non-complementing aggregateless mutations fell into five complementation groups (agoA, B, C, D and E) and a dominant aggregation class that allowed little or no aggregation when present in a diploid with any of the other mutations tested or the parental wild type. Complicating factors, including partial dominance, multiple mutations, and possible interallelic complementation, are discussed. Data on the linkage of the aggregateless mutations was obtained by using recessive drug resistance mutations on three linkage groups to segregate haploids from the diploids. Calculations from our results suggest a genetic complexity of about 50 genes that are specific and essential for aggregation.

scholarly journals Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture

1970 ◽  
Vol 119 (2) ◽  
pp. 171-174 ◽  
Author(s):  
D. J. Watts ◽  
J. M. Ashworth
Keyword(s):  
Cell Differentiation ◽  
Dictyostelium Discoideum ◽  
Axenic Culture ◽  
Axenic Medium ◽  
Cellular Slime Mould ◽  
Slime Mould ◽  
Solid Media ◽  
Cellular Slime

1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.

Control of cellular differentiation by temperature in the cellular slime mould Dictyostelium discoideum

1984 ◽  
Vol 69 (1) ◽  
pp. 159-165
Author(s):  
M. Maeda
Keyword(s):  
Low Temperature ◽  
Dictyostelium Discoideum ◽  
Cellular Differentiation ◽  
Cellular Slime Mould ◽  
Slime Mould ◽  
Cellular Slime

The effects of low temperature on morphogenesis and cellular differentiation of Dictyostelium discoideum were examined. During incubation at 5 degrees C, the vegetative and preaggregation cells never developed, but cell masses at the aggregation or slug stage developed to form hemispherical, or dumbbell-shaped multicellular structures. By staining with FITC-antispore IgG, the structures formed after 10 days of incubation of tipped aggregates at 5 degrees C were found to be composed of 90% spores, 5% prespore cells and 5% non-stained cells. Since only 20% of the total cells constituting the tipped aggregate had been prespore cells at the beginning of incubation, this showed that spore differentiation proceeded even at low temperature, while stalk differentiation was completely inhibited. Similar results were obtained when the cells were incubated at 3 degrees C. However, at 0 degree C, morphogenesis and cellular differentiation did not occur, although most of the prespore cells at the late culmination stage differentiated incompletely into spores. Possible reasons for the high proportion of spores being induced by low temperature are discussed.

Aggregation of the cellular slime mould Dictyostelium discoideum triggered by glucose starvation.

2008 ◽  
Vol 19and20 (1) ◽  
pp. 73-77
Author(s):  
Kazuhiro Ishigaki ◽  
Toshinori Usui ◽  
Tomoaki Abe
Keyword(s):  
Dictyostelium Discoideum ◽  
Glucose Starvation ◽  
Cellular Slime Mould ◽  
Slime Mould ◽  
Cellular Slime
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