Specific detection of RNA molecules by fluorescent in situ hybridization in living cells

1996 ◽  
Vol 12 (4-6) ◽  
pp. 359-361 ◽  
Author(s):  
S. Paillasson ◽  
M. Robert-Nicoud ◽  
X. Ronot
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Living Cells ◽  
Specific Detection ◽  
Rna Molecules

scholarly journals Specific Detection of Lawsonia intracellularis in Porcine Proliferative Enteropathy Inferred from Fluorescent rRNA In Situ Hybridization

1998 ◽  
Vol 35 (2) ◽  
pp. 153-156 ◽  
Author(s):  
M. Boye ◽  
T. K. Jensen ◽  
K. Møller ◽  
T. D. Leser ◽  
S. E. Jorsal
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Lawsonia Intracellularis ◽  
Specific Detection ◽  
Fixed Tissue ◽  
Tissue Samples ◽  
Formalin Fixed Tissue ◽  
Proliferative Enteropathy ◽  
Formalin Fixed

Fluorescent in situ hybridization targeting 16S ribosomal RNA was used for specific detection of the obligate intracellular bacterium Lawsonia intracellularis in enterocytes from pigs affected by proliferative enteropathy. A specific oligonucleotide probe was designed and the specificity of the probe was determined by simultaneous comparison with indirect immunofluorescence assay for detection of L. intracellularis in formalin-fixed tissue samples from 15 pigs affected by porcine proliferative enteropathy. We used 10 tissue samples from pigs without proliferative mucosal changes as negative controls. The results showed that the oligonucleotide probe is specific for L. intracellularis and that fluorescent in situ hybridization targeting ribosomal RNA is a suitable and fast method for specific detection and histological recognition of L. intracellularis in formalin-fixed tissue.

scholarly journals Specific Detection of Arcobacter and Campylobacter Strains in Water and Sewage by PCR and Fluorescent In Situ Hybridization

2003 ◽  
Vol 69 (2) ◽  
pp. 1181-1186 ◽  
Author(s):  
Yolanda Moreno ◽  
Salut Botella ◽  
José Luis Alonso ◽  
María A. Ferrús ◽  
Manuel Hernández ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Sequence Data ◽  
23S Rrna ◽  
Specific Detection ◽  
Specific Primers ◽  
Detection Range ◽  
Water And Wastewater ◽  
Wastewater Samples

ABSTRACT The aim of this study was to evaluate PCR and fluorescent in situ hybridization (FISH) techniques for detecting Arcobacter and Campylobacter strains in river water and wastewater samples. Both 16S and 23S rRNA sequence data were used to design specific primers and oligonucleotide probes for PCR and FISH analyses, respectively. In order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on selective media. The detection range of PCR and FISH assays varied between 1 cell/ml (after enrichment) to 103 cells/ml (without enrichment). According to our results, both rRNA-based techniques have the potential to be used as quick and sensitive methods for detection of campylobacters in environmental samples.

scholarly journals Specific detection of viable Listeria monocytogenes in Spanish wastewater treatment plants by Fluorescent In Situ Hybridization and PCR

2011 ◽  
Vol 45 (15) ◽  
pp. 4634-4640 ◽  
Author(s):  
Yolanda Moreno ◽  
Lorena Ballesteros ◽  
Jorge García-Hernández ◽  
Paula Santiago ◽  
Ana González ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
In Situ Hybridization ◽  
Listeria Monocytogenes ◽  
Fluorescent In Situ Hybridization ◽  
Wastewater Treatment Plants ◽  
Specific Detection
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