Ethanol and biomass production of wild strains and respiratory deficient mutants of Saccharomyces cerevisiae under anaerobic and aerobic conditions

1982 ◽  
Vol 16 (2-3) ◽  
pp. 161-164 ◽  
Author(s):  
Karl Esser ◽  
Udo Schmidt ◽  
Ulf Stahl
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Biomass Production ◽  
Aerobic Conditions ◽  
Wild Strains ◽  
Respiratory Deficient

scholarly journals Electromagnetic Fields (0.04 to 0.39) mT effect on cellular growth cycles of Saccharomyces cerevisiae wine strains

2019 ◽  
Vol 17 (2) ◽  
pp. 196
Author(s):  
Eliseo Amado-González ◽  
Alveiro Álvarez Ovallos ◽  
Alfonso Quijano Parra
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Electromagnetic Fields ◽  
Biomass Production ◽  
Exposure Time ◽  
Culture Media ◽  
Dry Weight ◽  
Cellular Growth ◽  
Biomass Growth ◽  
Yeast Saccharomyces Cerevisiae ◽  
Growth Cycles

Low frecuency electromagnetic fields effect (EMF) on growth cycles of yeast Saccharomyces cerevisiae wine strains Rv1 and Rhône were studied.  A cylindrical coil induced magnetic fields with inductions up to 0,39 mT. Exposure time to EMF varied between (1 – 10) min at 30 °C.  The biomass growth were monitored in the reactor culture media (yeast extract + by measurement optical density from (0 to 32) h. The biomass was found by dry weight. After yeast expose to the different EMF, the number of growth cycles decreased from 4 cycles to 2 or 1. However, the biomass production increased almost 50 %.  The best biomass production was found at 0.39 mT and 10 min exposure time.  Keywords: Electromagnetic fields, Saccharomyces cerevisiae, biomass production, RV1

REVERSION OF SPONTANEOUSLY ARISING RESPIRATORY DEFICIENCY INSACCHAROMYCES CEREVISIAE

1969 ◽  
Vol 11 (3) ◽  
pp. 716-728 ◽  
Author(s):  
Gerald H. Rank ◽  
Clayton Person
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Dna ◽  
Genetic Information ◽  
Respiratory Deficiency ◽  
Respiratory Deficient

Reversion of naturally-arising cytoplasmically-inherited respiratory deficiency in Saccharomyces cerevisiae was indicated by the occurrence of colonies with a respiratory sufficient apex arising from a respiratory deficient base. The basal respiratory deficient cells were shown to contain the suppressive factor. It was suggested that genetic information for the suppressive factor resided in abnormal mitochondrial DNA and that mosaic colonies arose from a heteroplasmic cell containing both normal and abnormal mitochondrial DNA.

A novel metabolite producing respiratory deficient phenocopies in Saccharomyces cerevisiae

1976 ◽  
Vol 7 (2) ◽  
pp. 109-117 ◽  
Author(s):  
G.Lucchini Bonomini ◽  
C. Frova ◽  
L. Panzeri ◽  
M. Paternoster ◽  
M. Cocucci
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Respiratory Deficient

Regulation of phosphoenolpyruvate carboxykinase and pyruvate kinase in Saccharomyces cerevisiae grown in the presence of glycolytic and gluconeogenic carbon sources and the role of mitochondrial function on gluconeogenesis

1986 ◽  
Vol 32 (12) ◽  
pp. 969-972 ◽  
Author(s):  
Albert J. Wilson ◽  
J. K. Bhattacharjee
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pyruvate Kinase ◽  
Growth Medium ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Carbon Sources ◽  
Absolute Requirement ◽  
Fructose 1,6 Bisphosphatase ◽  
Futile Cycling ◽  
Respiratory Deficient

Phosphoenolpyruvate carboxykinase (PEPCKase) and pyruvate kinase (PKase) were measured in Saccharomyces cerevisiae grown in the presence of glycolytic and gluconeogenic carbon sources. The PEPCKase activity was highest in ethanol-grown cells. However, high PEPCKase activity was also observed in cells grown in 1% glucose, especially as compared with the activity of sucrose-, maltose-, or galactose-grown cells. Activity was first detected after 12 h when glucose was exhausted from the growth medium. The PKase activity was very high in glucose-grown cells; considerable activity was also present in ethanol- and pyruvate-grown cells. The absolute requirement of respiration for gluconeogenesis was demonstrated by the absence or significantly low levels of PEPCKase and fructose-1,6-bisphosphatase activities observed in respiratory deficient mutants, as well as in wild-type S. cerevisiae cells grown in the presence of glucose and antimycin A or chloramphenicol. Obligate glycolytic and gluconeogenic enzymes were present sumultaneously only in stationary phase cells, but not in exponential phase cells; hence futile cycling could not occur in log phase cells regardless of the presence of carbon source in the growth medium.

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