Ethanol and glycerol production under aerobic conditions by wild-type, respiratory-deficient mutants and a fusion product of Saccharomyces cerevisiae

1984 ◽  
Vol 20 (2) ◽  
Author(s):  
Marianne Johansson ◽  
Jan-Eric Sj�str�m
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fusion Product ◽  
Wild Type ◽  
Glycerol Production ◽  
Aerobic Conditions ◽  
Respiratory Deficient

scholarly journals Anaerobicity Prepares Saccharomyces cerevisiae Cells for Faster Adaptation to Osmotic Shock

2004 ◽  
Vol 3 (6) ◽  
pp. 1381-1390 ◽  
Author(s):  
Marcus Krantz ◽  
Bodil Nordlander ◽  
Hadi Valadi ◽  
Mikael Johansson ◽  
Lena Gustafsson ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Osmotic Shock ◽  
Production Capacity ◽  
Transcriptional Response ◽  
Global Gene Expression ◽  
Yeast Cells ◽  
Glycerol Production ◽  
Gene Expression Response ◽  
Aerobic Conditions

ABSTRACT Yeast cells adapt to hyperosmotic shock by accumulating glycerol and altering expression of hundreds of genes. This transcriptional response of Saccharomyces cerevisiae to osmotic shock encompasses genes whose products are implicated in protection from oxidative damage. We addressed the question of whether osmotic shock caused oxidative stress. Osmotic shock did not result in the generation of detectable levels of reactive oxygen species (ROS). To preclude any generation of ROS, osmotic shock treatments were performed in anaerobic cultures. Global gene expression response profiles were compared by employing a novel two-dimensional cluster analysis. The transcriptional profiles following osmotic shock under anaerobic and aerobic conditions were qualitatively very similar. In particular, it appeared that expression of the oxidative stress genes was stimulated upon osmotic shock even if there was no apparent need for their function. Interestingly, cells adapted to osmotic shock much more rapidly under anaerobiosis, and the signaling as well as the transcriptional response was clearly attenuated under these conditions. This more rapid adaptation is due to an enhanced glycerol production capacity in anaerobic cells, which is caused by the need for glycerol production in redox balancing. Artificially enhanced glycerol production led to an attenuated response even under aerobic conditions. These observations demonstrate the crucial role of glycerol accumulation and turgor recovery in determining the period of osmotic shock-induced signaling and the profile of cellular adaptation to osmotic shock.

scholarly journals The ROX3 gene encodes an essential nuclear protein involved in CYC7 gene expression in Saccharomyces cerevisiae.

1991 ◽  
Vol 11 (11) ◽  
pp. 5639-5647 ◽  
Author(s):  
L S Rosenblum-Vos ◽  
L Rhodes ◽  
C C Evangelista ◽  
K A Boayke ◽  
R S Zitomer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fold Increase ◽  
Fusion Product ◽  
Wild Type ◽  
Coding Region ◽  
Yeast Saccharomyces Cerevisiae ◽  
Coding Sequence ◽  
Amino Terminal ◽  
Terminal Domain ◽  
Carboxy Terminal

The ROX3 gene was identified during a hunt for mutants with increased expression of the heme-regulated CYC7 gene, which encodes the minor species of cytochrome c in the yeast Saccharomyces cerevisiae. The rox3 mutants caused a 10-fold increase in CYC7 expression both in the presence and absence of heme, had slightly increased anaerobic expression of the heme-activated CYC1 gene, and caused decreases in the anaerobic expression of the heme-repressed ANB1 gene and the aerobic expression of its heme-induced homolog. The wild-type ROX3 gene was cloned, and the sequence indicated that it encodes a 220-amino-acid protein. This protein is essential; deletion of the coding sequence was lethal. The coding sequence for beta-galactosidase was fused to the 3' end of the ROX3 coding sequence, and the fusion product was found to be localized in the nucleus, strongly suggesting that the wild-type protein carries out a nuclear function. Mutations in the rox3 gene showed an interesting pattern of intragenic complementation. A deletion of the 5' coding region complemented a nonsense mutation at codon 128 but could not prevent the lethality of the null mutation. These results suggest that the amino-terminal domain is required for an essential function, while the carboxy-terminal domain can be supplied in trans to achieve the wild-type expression of CYC7. Finally, RNA blots demonstrated that the ROX3 mRNA was expressed at higher levels anaerobically but was not subject to heme repression. The nuclear localization and the lack of viability of null mutants suggest that the ROX3 protein is a general regulatory factor.

The ROX3 gene encodes an essential nuclear protein involved in CYC7 gene expression in Saccharomyces cerevisiae

1991 ◽  
Vol 11 (11) ◽  
pp. 5639-5647
Author(s):  
L S Rosenblum-Vos ◽  
L Rhodes ◽  
C C Evangelista ◽  
K A Boayke ◽  
R S Zitomer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fold Increase ◽  
Fusion Product ◽  
Wild Type ◽  
Coding Region ◽  
Yeast Saccharomyces Cerevisiae ◽  
Coding Sequence ◽  
Amino Terminal ◽  
Terminal Domain ◽  
Carboxy Terminal

The ROX3 gene was identified during a hunt for mutants with increased expression of the heme-regulated CYC7 gene, which encodes the minor species of cytochrome c in the yeast Saccharomyces cerevisiae. The rox3 mutants caused a 10-fold increase in CYC7 expression both in the presence and absence of heme, had slightly increased anaerobic expression of the heme-activated CYC1 gene, and caused decreases in the anaerobic expression of the heme-repressed ANB1 gene and the aerobic expression of its heme-induced homolog. The wild-type ROX3 gene was cloned, and the sequence indicated that it encodes a 220-amino-acid protein. This protein is essential; deletion of the coding sequence was lethal. The coding sequence for beta-galactosidase was fused to the 3' end of the ROX3 coding sequence, and the fusion product was found to be localized in the nucleus, strongly suggesting that the wild-type protein carries out a nuclear function. Mutations in the rox3 gene showed an interesting pattern of intragenic complementation. A deletion of the 5' coding region complemented a nonsense mutation at codon 128 but could not prevent the lethality of the null mutation. These results suggest that the amino-terminal domain is required for an essential function, while the carboxy-terminal domain can be supplied in trans to achieve the wild-type expression of CYC7. Finally, RNA blots demonstrated that the ROX3 mRNA was expressed at higher levels anaerobically but was not subject to heme repression. The nuclear localization and the lack of viability of null mutants suggest that the ROX3 protein is a general regulatory factor.

Improvement in ethanol production using respiratory deficient phenotype of a wild type yeast Saccharomyces cerevisiae ITV-01

2012 ◽  
Vol 37 (1) ◽  
pp. 197-201 ◽  
Author(s):  
B. Ortiz-Muñiz ◽  
O. Carvajal-Zarrabal ◽  
B. Aguilar ◽  
M.G. Aguilar-Uscanga
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Wild Type ◽  
Yeast Saccharomyces Cerevisiae ◽  
Wild Type Yeast ◽  
Respiratory Deficient

Transcript analysis of 203 novel genes from Saccharomyces cerevisiae in hap1 and rox1 mutant backgrounds

Genome ◽  
2000 ◽  
Vol 43 (5) ◽  
pp. 881-886 ◽  
Author(s):  
L J Lombardía ◽  
J L Cadahía-Rodríguez ◽  
M A Freire-Picos ◽  
M I González-Siso ◽  
A M Rodríguez-Torres ◽  
...  
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Transcriptional Regulators ◽  
Open Reading Frames ◽  
Transcript Analysis ◽  
Wild Type ◽  
Aerobic Conditions ◽  
The Family ◽  
Reading Frames ◽  
Regulatory Sites

Hap1 and Rox1 are transcriptional regulators that bind regulatory sites in the promoters of oxygen-regulated genes in Saccharomyces cerevisiae. Hap1 is a heme-responsive activator of genes induced in aerobic conditions and Rox1 is a repressor of hypoxic genes in aerobic conditions. We have studied transcriptional regulation of a pool of 203 open reading frames (ORFs) from chromosomes IV, VII, and XIV in wild-type, hap1, and rox1 mutant genetic backgrounds in an attempt to extend the family of oxygen and heme regulated genes. Only three ORFs are significantly repressed by Rox1 but they cannot be considered as typical hypoxic genes because they are not overexpressed during hypoxia.Key words: Saccharomyces cerevisiae, genome analysis, chromosomes IV, VII, and XIV, gene expression, ROX1 and HAP1.

scholarly journals Effects of Mutations of RAD50, RAD51, RAD52, and Related Genes on Illegitimate Recombination in Saccharomyces cerevisiae

Genetics ◽  
1996 ◽  
Vol 142 (2) ◽  
pp. 383-391 ◽  
Author(s):  
Yasumasa Tsukamoto ◽  
Jun-ichi Kato ◽  
Hideo Ikeda
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Quantitative Analysis ◽  
Structural Analysis ◽  
Recombination Rate ◽  
Type Strain ◽  
Negative Selection ◽  
Wild Type Strain ◽  
Illegitimate Recombination ◽  
Wild Type ◽  
In The Wild

Abstract To examine the mechanism of illegitimate recombination in Saccharomyces cerevisiae, we have developed a plasmid system for quantitative analysis of deletion formation. A can1 cyh2 cell carrying two negative selection markers, the CAN1 and CYH2 genes, on a YCp plasmid is sensitive to canavanine and cycloheximide, but the cell becomes resistant to both drugs when the plasmid has a deletion over the CAN1 and CYH2 genes. Structural analysis of the recombinant plasmids obtained from the resistant cells showed that the plasmids had deletions at various sites of the CAN1-CYH2 region and there were only short regions of homology (1-5 bp) at the recombination junctions. The results indicated that the deletion detected in this system were formed by illegitimate recombination. Study on the effect of several rad mutations showed that the recombination rate was reduced by 30-, 10-, 10-, and 10-fold in the rad52, rad50, mre11, and xrs2 mutants, respectively, while in the rud51, 54, 55, and 57 mutants, the rate was comparable to that in the wild-type strain. The rad52 mutation did not affect length of homology at junction sites of illegitimate recombination.

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