The pharmacokinetic properties of a new non-steroidal anti-inflammatory preparation of nabumetone have been studied in both plasma and extravascular fluids. The skin suction blister technique was used on six healthy volunteers on two separate occasions and gave good, reproducible results. A single oral dose of 1 g nabumetone achieved significant blood and skin suction blister fluid concentrations. The partition index (area under the concentration curve for the skin suction blister/area under the concentration curve for blood) showed that the orally administered drug was well diffused within the body.
Abstract
A method is described for determination of 8-methoxypsoralen to 0.2 mL of suction-blister fluid or 1 mL of serum from psoriatic patients being treated with this drug. The drug is extracted from the biological matrix at pH 9.0 with a mixture of dichloromethane and light petroleum ether. 5-Methoxypsoralen is used as internal standard. Separation and quantitation are performed on a “high-performance” liquid chromatograph with use of an RP 18 reversed-phase column and detection at 245 nm. Accuracy and precision are good. Some benzodiazepines and their metabolites interfere. The lowest detectable concentration is 10 microgram/L, which means that the method is sufficiently sensitive to measure the drug concentrations in serum and suction-blister fluid for pharmacokinetic studies in patients being treated with a therapeutic dosage.
Currents: Searching the signaling subproteome for Alzheimer's biomarkers | Pseudo-internal standards for label-free quantitation | Top-down proteomics on a chromatographic timescale | Proteomics of suction blister fluid | Absolute quantitation with PSAQ | SILAC for top-down MS | HUPO BPP pilot-study results