Molecular cloning of the imidazoleglycerolphosphate dehydratase gene of Trichoderma harzianum by genetic complementation in Saccharomyces cerevisiae using a direct expression vector

1992 ◽  
Vol 234 (3) ◽  
pp. 481-488 ◽  
Author(s):  
G. H. Goldman ◽  
J. Demolder ◽  
S. Dewaele ◽  
A. Herrera-Estrella ◽  
R. A. Geremia ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Cloning ◽  
Trichoderma Harzianum ◽  
Expression Vector ◽  
Genetic Complementation

scholarly journals Expression of GA Coat Protein-Derived Mosaic Virus-Like Particles in Saccharomyces cerevisiae and Packaging in vivo of mRNAs into Particles

2012 ◽  
Vol 66 (6) ◽  
pp. 234-241
Author(s):  
Arnis Strods ◽  
Dagnija Argule ◽  
Indulis Cielens ◽  
Ludmila Jackeviča ◽  
Regīna Renhofa
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Coat Protein ◽  
Pichia Pastoris ◽  
Mosaic Virus ◽  
Expression Vector ◽  
Expression Systems ◽  
Coding Sequences ◽  
Yeast Pichia Pastoris ◽  
Virus Like Particles

Our previous research showed that the best yield of virus-like particles (VLPs) formed by RNA bacteriophage GA coat protein was obtained by expression in yeast Pichia pastoris, while other used expression systems in Saccharomyces cerevisiae gave much lower amounts of capsids. The main reasons to attempt further studies in Saccharomyces cerevisiae were to improve the yield of GA-based VLPs using constructs with optimised nucleotide triplets in coding sequences, and to exploit the possibilities of the two-promoter Gal1/Gal10 system of expression vector pESC-URA for production of the desired mosaic VLPs and for packaging of mRNAs into VLPs in vivo

scholarly journals Molecular cloning and transcriptional analysis of the start gene CDC25 of Saccharomyces cerevisiae

1986 ◽  
Vol 5 (9) ◽  
pp. 2363-2369 ◽  
Author(s):  
Enzo Martegani ◽  
Maurizio D. Baroni ◽  
Gianni Frascotti ◽  
Lilia Alberghina
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Cloning ◽  
Transcriptional Analysis
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