Mapping of mutationtsm-8 with respect to transfer RNA genes on the mitochondrial DNA ofSaccharomyces cerevisiae

In this study, the complete mitochondrial DNA sequence of Parum colligata (Lepidoptera: Sphingidae: Smerinthinae) was sequenced firstly. The mitogenome is 15,288 bp in size, containing 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and an A+T-rich region. In the mitogenome, Ile, Leu2, and Phe are the most frequently used codon families, while codons GCG, TGC, GGC, CTG, AGG, and ACG are absent. The A+T-rich region is 358 bp in length including a motif ‘ATAGA’, an 18 bp poly-T stretch, three copies of a 12 bp ‘TATATATATATA’, and a short poly-A element. The nucleotides sequence of A+T-rich region is closer to Sphinginae than Macroglossinae. Phylogenetic analyses, based on the PCGs by using Maximum Likelihood (ML) and Bayesian Inference (BI) methods, generated consistent results that Smerinthinae was clustered together with Sphinginae to be the sister groups rather than Macroglossinae.

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Transfer RNA genes in the mitochondrial DNA of cytoplasmic petite mutants of Saccharomyces cerevisiae

Journal of Molecular Biology ◽

10.1016/0022-2836(74)90395-7 ◽

1974 ◽

Vol 88 (4) ◽

pp. 717-733 ◽

Cited By ~ 35

Author(s):

James W. Casey ◽

Huey-Juang Hsu ◽

Murray Rabinowitz ◽

Godfrey S. Getz ◽

Hiroshi Fukuhara

Keyword(s):

Saccharomyces Cerevisiae ◽

Mitochondrial Dna ◽

Transfer Rna ◽

Petite Mutants ◽

Rna Genes

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Nucleotide sequence of transfer RNA genes from the linear mitochondrial DNA of the yeastWilliopsis mrakiiandPichia pijperi

Nucleic Acids Research ◽

10.1093/nar/20.13.3509 ◽

1992 ◽

Vol 20 (13) ◽

pp. 3509-3509 ◽

Cited By ~ 3

Author(s):

Nathalie Dinouël ◽

Frédéric Sor ◽

Hiroshi Fukuhara

Keyword(s):

Mitochondrial Dna ◽

Nucleotide Sequence ◽

Transfer Rna ◽

Linear Mitochondrial Dna ◽

Rna Genes

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Complete mitochondrial DNA sequence analysis of Bluespotted seabream, Pagrus caeruleostictus (Perciformes: Sparidae)

10.21203/rs.2.16176/v2 ◽

2019 ◽

Author(s):

Gyamfua Afriyie ◽

Yusong Guo ◽

Felix K.A Kuebutornye ◽

Christian Ayisi Larbi ◽

Zhongduo Wang

Keyword(s):

Mitochondrial Dna ◽

Dna Sequence ◽

Molecular Genetic ◽

Marine Species ◽

Illumina Hiseq ◽

Protein Coding ◽

Tropical Regions ◽

Mitochondrial Dna Sequence ◽

Rna Genes ◽

Pagrus Caeruleostictus

Abstract Background Bluespotted seabream, Pagrus caeruleostictus is a benthopelagic marine species found in tropical regions and a member of the family Sparidae and genus Pagrus . In this article, a mitochondrial DNA sequence by high-throughput technique, Illumina Hiseq, was carried out on muscle of the above species and determined the complete mitogenome. Samples were obtained from species collected from the coast of Ghana, West Africa.Results The complete mitochondrial DNA sequence was 16,653 bases pairs in length (GenBank Accession number: MN319701) and comprises of 37 genes; 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes as well as a control region as in a typical vertebrate mitochondrial DNA gene. The phylogenetic analysis showed P. caeruleostictus clustered with the clade of P. auriga. Conclusion This research will serve as the foundation for molecular genetic studies of Ghanaian fish species. Moreover, it will contribute to the phylogenetics of class Actinopterygii, order Spariformes, family Sparidae and genus Pagrus.

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The complete mitochondrial genome and description of a new cryptic species of Benedenia Diesing, 1858 (Monogenea: Capsalidae), a major pathogen infecting the yellowtail kingfish Seriola lalandi Valenciennes in the South-East Pacific

Parasites & Vectors ◽

10.1186/s13071-019-3711-5 ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 4

Author(s):

J. Antonio Baeza ◽

Fabiola A. Sepúlveda ◽

M. Teresa González

Keyword(s):

Mitochondrial Genome ◽

Ribosomal Rna ◽

Complete Mitochondrial Genome ◽

Transfer Rna ◽

The South ◽

Protein Coding ◽

East Pacific ◽

Yellowtail Kingfish ◽

Seriola Lalandi ◽

Rna Genes

Abstract Background The monogenean Benedenia seriolae parasitizes fishes belonging to the genus Seriola, represents a species complex, and causes substantial impact on fish welfare in aquaculture systems worldwide. This study reports, for the first time, the complete mitochondrial genome of B. humboldti n. sp., a new cryptic species from the South-East Pacific (SEP). Methods The mitogenome of B. humboldti n. sp. was assembled from short Illumina 150 bp pair-end reads. The phylogenetic position of B. humboldti n. sp. among other closely related congeneric and confamiliar capsalids was examined using mitochondrial protein-coding genes (PCGs). Morphology of B. humboldti n. sp. was examined based on fixed and stained specimens. Results The AT-rich mitochondrial genome of B. humboldti is 13,455 bp in length and comprises 12 PCGs (atp8 was absent as in other monogenean genomes), 2 ribosomal RNA genes, and 22 transfer RNA genes. All protein-coding, ribosomal RNA, and transfer RNA genes are encoded on the H-strand. The gene order observed in the mitochondrial genome of B. humboldti n. sp. was identical to that of B. seriolae from Japan but different from that of B. seriolae from Australia. The genetic distance between B. humboldti n. sp. and B. seriolae from Japan was high. Minor but reliable differences in the shape of the penis were observed between Benedenia humboldti n. sp. and congeneric species. Conclusions Phylogenetic analyses based on PCGs in association with differences in the shape of the penis permitted us to conclude that the material from the South-East Pacific represents a new species of Benedenia infecting S. lalandi off the coast of Chile. The discovery of this parasite represents the first step to improving our understanding of infestation dynamics and to develop control strategies for this pathogen infecting the farmed yellowtail kingfish, Seriola lalandi, in the South-East Pacific.

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