Modulation of oligosaccharide structure of a pro-urokinase derivative (pro-UK?GS1) by changing culture conditions of a lymphoblastoid cell line Namalwa KJM-1 adapted to serum-free medium

1996 ◽  
Vol 19 (2) ◽  
pp. 125-135
Author(s):  
Shinji Hosoi ◽  
Mitsuo Satoh ◽  
Katsuya Higo ◽  
Seiji Sugimoto ◽  
Hiromasa Miyaji ◽  
...  
Keyword(s):  
Cell Line ◽  
Lymphoblastoid Cell Line ◽  
Culture Conditions ◽  
Serum Free Medium ◽  
Free Medium ◽  
Oligosaccharide Structure ◽  
Serum Free

scholarly journals The effects of various hormones and growth factors on the growth of human insulin-producing cell line in serum-free medium

1997 ◽  
Vol 29 (4) ◽  
pp. 209-216 ◽  
Author(s):  
Jae-Jeong Lee ◽  
Jai-Hyun Kwon ◽  
Yong Keun Park ◽  
Ohoak Kwon ◽  
Tai-Wook Yoon
Keyword(s):  
Growth Factors ◽  
Cell Line ◽  
Human Insulin ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

scholarly journals Rational development of a serum-free medium and fed-batch process for a GS-CHO cell line expressing recombinant antibody

2012 ◽  
Vol 65 (3) ◽  
pp. 363-378 ◽  
Author(s):  
Huifeng Zhang ◽  
Haibin Wang ◽  
Mei Liu ◽  
Tao Zhang ◽  
Ji Zhang ◽  
...  
Keyword(s):  
Cell Line ◽  
Recombinant Antibody ◽  
Batch Process ◽  
Serum Free Medium ◽  
Free Medium ◽  
Fed Batch ◽  
Cho Cell ◽  
Serum Free ◽  
Rational Development ◽  
Cho Cell Line

scholarly journals Mutant strain of Chinese hamster ovary cells with no detectable ornithine decarboxylase activity.

1985 ◽  
Vol 5 (6) ◽  
pp. 1385-1390 ◽  
Author(s):  
P Pohjanpelto ◽  
E Hölttä ◽  
O A Jänne
Keyword(s):  
Cell Line ◽  
Mutant Strain ◽  
Ornithine Decarboxylase ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Decarboxylase Activity ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Mutant Cells

We previously described an arginase-deficient, polyamine-dependent Chinese hamster ovary cell line which grows in serum-free medium. From this strain we isolated a new mutant strain that has no detectable catalytic ornithine decarboxylase activity. The mutant cells contain, however, immunoreactive ornithine decarboxylase-like protein roughly in the same quantity as the parent strain. The mutant and the parent cell line strains also contain similar amounts of ornithine decarboxylase-mRNA hybridizable to a specific cDNA. If polyamines are omitted from the medium, proliferation of the mutant cells is considerably retarded and ceases in 6 to 10 days. Addition of ornithine or alpha-difluoromethylornithine, a specific inhibitor of ornithine decarboxylase, has no effect on these cells. Putrescine and spermidine decreased in the mutant cells to undetectable levels during polyamine starvation, whereas spermine was reduced to 1/5th of that found in the control cultures. Polyamines appear to be indispensable for the mutant strain, but this was obvious only after the amount of polyamines, found as impurities in bovine serum albumin used in the medium, was reduced by dialysis to 10(-12) M. Because sera contain polyamines, the ability of the mutant strain to grow in serum-free medium is a great advantage in elucidation of the mechanisms of polyamine function.

Proliferation of a human embryonal carcinoma-derived cell line in serum-free medium: inter-relationship between growth factor requirements and membrane receptor expression

1985 ◽  
Vol 73 (1) ◽  
pp. 361-373
Author(s):  
W. Engstrom ◽  
A.R. Rees ◽  
J.K. Heath
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Embryonal Carcinoma ◽  
Density Lipoprotein ◽  
Basal Medium ◽  
Receptor Expression ◽  
Embryonal Carcinoma Cell ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Substantial multiplication in vitro of cloned cells from a human embryonal carcinoma cell line, Tera 2, has been obtained in a basal medium (DMEM/Ham's F12,50:50, v/v) supplemented with 10 micrograms low density lipoprotein/ml, 100 micrograms high density lipoprotein/ml, 100 ng multiplication stimulating activity/ml, 100 ng insulin/ml and 1 microgram transferrin/ml. The growth rate appears to be similar to that obtained in 10% serum. Furthermore, studies on the expression of cell surface receptors revealed that cloned Tera 2 cells express high-affinity receptors for IGF-II but not for insulin. The cells also express receptors for Epidermal Growth Factor (EGF) even though the addition of EGF does not stimulate their proliferation in serum-free medium. These results suggest that the expression of specific growth factor receptors is not an absolute determinant of hormone responsiveness.

Mutant strain of Chinese hamster ovary cells with no detectable ornithine decarboxylase activity

1985 ◽  
Vol 5 (6) ◽  
pp. 1385-1390
Author(s):  
P Pohjanpelto ◽  
E Hölttä ◽  
O A Jänne
Keyword(s):  
Cell Line ◽  
Mutant Strain ◽  
Ornithine Decarboxylase ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Decarboxylase Activity ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Mutant Cells

We previously described an arginase-deficient, polyamine-dependent Chinese hamster ovary cell line which grows in serum-free medium. From this strain we isolated a new mutant strain that has no detectable catalytic ornithine decarboxylase activity. The mutant cells contain, however, immunoreactive ornithine decarboxylase-like protein roughly in the same quantity as the parent strain. The mutant and the parent cell line strains also contain similar amounts of ornithine decarboxylase-mRNA hybridizable to a specific cDNA. If polyamines are omitted from the medium, proliferation of the mutant cells is considerably retarded and ceases in 6 to 10 days. Addition of ornithine or alpha-difluoromethylornithine, a specific inhibitor of ornithine decarboxylase, has no effect on these cells. Putrescine and spermidine decreased in the mutant cells to undetectable levels during polyamine starvation, whereas spermine was reduced to 1/5th of that found in the control cultures. Polyamines appear to be indispensable for the mutant strain, but this was obvious only after the amount of polyamines, found as impurities in bovine serum albumin used in the medium, was reduced by dialysis to 10(-12) M. Because sera contain polyamines, the ability of the mutant strain to grow in serum-free medium is a great advantage in elucidation of the mechanisms of polyamine function.

Ex Vivo Expansion of Human Marrow Mesenchymal Stem Cells: A Serum-Free Medium Compared to Classical α-MEM Medium.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4257-4257 ◽  
Author(s):  
Nathalie Meuleman ◽  
Tatiana Tondreau ◽  
Alain Delforge ◽  
Marielle Dejeneffe ◽  
Martine Massy ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Culture Conditions ◽  
Adherent Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Marrow Mesenchymal Stem Cells ◽  
Serum Substitute ◽  
Cells Cultured

Abstract BACKGROUND: Bone Marrow mesenchymal stem cells (MSC) are pluripotent cells that have the capacity to differentiate into several tissue lineages and also the ability to support hematopoiesis. Their use in gene and cell therapy requires their in vitro expansion. Maintenance and proliferation of MSC are strongly dependent on the culture conditions such as properties of the bovine serum added in the culture medium. However, duration and culture conditions are critical for the successful clinical use of MSC. OBJECTIVE: We have evaluated the efficiency of a commercial serum-free medium (UltraCulture, Bio-Whittaker, Walkersville, MD) supplemented with a serum substitute (Ultroser, BioSepra, Cergy-Saint-Christophe, France) in order to work without FBS and to have a more constant composition. This medium (UC) was compared to the classical medium a-MEM containing 10% FBS (Invitrogen, Merelbeke, Belgium). METHODS: BM-mononuclear cells collected from 11 healthy donors were plated in petri dishes at a concentration of 105/cm2. After 3 days, non adherent cells were removed and culture media were added to adherent cells which were maintained at 37°C until they reached confluence. MSC expansion was analysed after the primoculture (PM) and after the first passage (P1). CFU-F (colony forming units-fibroblastic) number, phenotypic analysis and differentiation potential were also evaluated. RESULTS::The mean culture duration was 13±2 and 8±2 days respectively for PM and P1 but the confluence was reached more rapidly for cells cultured in UC. After PM, 0.35x106 and 1.21x106 cells were obtained for MEM and UC respectively (p<0.005). Moreover, around 20% of cells cultured in MEM were CD45+ while the level of CD45+ cells was frequently < 5% in UC indicating that UC medium favored the rapid elimination of hematopoietic cells. After P1, the expansion rate was significantly higher in UC than MEM: respectively 5.13 ± 1.2 and 22.6 ± 3 (p<0.0005). The numbers of CFU-F were always higher in UC demonstrating the enhanced proliferation in serum-free medium. The phenotype of MSC was similar in the both media: SH2+, SH3+, CD44+, CD45−, CD34−, HLA-DR−. More importantly, these cells remained able to differentiate into osteocytes, chondrocytes, adipocytes and neuron-like cells in the both media confirming their multipotentiality. CONCLUSIONS: Our data strongly support UltraCulture medium supplemented with a serum substitute as an optimal medium for MSC culture. Indeed, it allows a better cell expansion, better proliferation and preserves multipotentiality. This medium, reducing culture period and containing low concentration of serum substitute, is of major interest for clinical production of MSC.

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