Amide- and urea-based synthetic anticonvulsants, antihypoxics, and inducers of the hepatic monooxygenase system. IX. Synthesis and search for inducers of the liver cytochrome P-450-dependent monooxygenase system among carbamide-containing heterocyclic compounds

The hepatic monooxygenase system was studied in hypophysectomized female rats infused for 5 days with ovine growth hormone (GH). At 7.5 μg∙h−1 GH decreased the total cytochrome P-450 by 16%; at 10 μg∙h−1 it reduced both cytochrome P-450 (31%) and the activity of ethylmorphine demethylase (31%). GH did not alter the activities of NADPH cytochrome c reductase or aniline hydroxylase. The lower GH dose decreased the amount of fast- and slow-turnover P-450 by 11 and 38%, respectively, while the higher dose decreased both by 49%. The loss of demethylase activity therefore correlates with the loss of fast-tumover P-450. This component is relatively more abundant in the female (fast: slow turnover of 4.3) than the male (fast: slow turnover of 2.5). GH did not affect the half-lives of the P-450 components, suggesting that it decreases their synthesis. The P-450 concentration in microsomes from GH-treated animals did not increase after incubation with hemin, suggesting that in vivo the hormone does not lower P-450 synthesis via depression of heme. Puromycin mimicked the effect of GH and when given with the hormone their effects on the P-450 levels were multiplicative (p < 0.05), suggesting different modes of action and that GH does not decrease P-450 by acting at translation.

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THE EFFECT OF VITAMIN E ON THE BIOCHEMICAL PARAMETERS IN THE EXPERIMENT

Doctor's Herald/ Доктор ахборотномаси /Вестник врача ◽

10.38095/2181-466x-2020931-103-105 ◽

2020 ◽

Vol 93 (1) ◽

pp. 103-105

Author(s):

I. Shukurov ◽

◽

Ch. Khayrullayev ◽

M. Gulomova ◽

F. Umurov

Keyword(s):

Acute Pancreatitis ◽

Cytochrome P450 ◽

Vitamin E ◽

Rat Liver ◽

Biochemical Parameters ◽

Microsomal Fraction ◽

Monooxygenase System ◽

Experimental Acute Pancreatitis ◽

Liver Cytochrome ◽

Cytochrome P 450

The effect of vitamin E on rat liver cytochrome P-450 in experimental acute pancreatitis (AP) was studied. The animals were divided into 4 groups. The obtained data were compared with the indicators of the 1–st group (intact). During the experiment, the development of AP showed a decrease in the content of cytochrome P450 in the microsomal fraction. The administration of vitamin E to animals of the 4th group led to an increase in the content of cytochrome P-450, strengthening the protection of the liver, the abolition of inhibition of the monooxygenase system of the liver.

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Benzobarbital and fluorbenzobarbital - hepatic monooxygenase system phenobarbital-like inducers

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2011-5-78-81 ◽

2011 ◽

Vol 10 (5) ◽

pp. 78-81 ◽

Cited By ~ 1

Author(s):

T. P. Novozheyeva ◽

M. I. Smagina ◽

N. A. Cherevko ◽

S. N. Fateyeva

Keyword(s):

Large Degree ◽

Major Metabolite ◽

Monooxygenase System ◽

Oxidation Activity ◽

Hepatic Monooxygenase ◽

Cytochrome P 450 ◽

Hepatic Cytochrome

Benzobarbital and fluorbenzobarbital as monooxygenase system inductors increase the hepatic cytochrome P-450 level and the content of its isoenzymes 2B6, 2C9, 2Å1, accelerate aminopyrine, 7-ethoxyresorufine, 7-pentoxyresorufine, aniline and androstendione oxidation. Activity of benzobarbital and fluorbenzobarbital as inductors is to a large degree due to the action of their major metabolite — phenobarbital. Benzobarbital and fluorbenzobarbital unlike phenobarbital induce isoenzyme 3A4, responsible for androstendione 16b-OH-hydroxylation. PCN-type induction activity posses also native molecules of benzobarbital and fluorbenzobarbital.

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