Changes in erythrocyte count and hemoglobin content in the peripheral blood under prolonged nociceptive irritation

1957 ◽  
Vol 43 (2) ◽  
pp. 156-160
Author(s):  
V. V. Kravtsov
Author(s):  
V. Holovakha ◽  
E. Mostovoy ◽  
O. Piddubnyak ◽  
О. Kurchev

In our studies, we examined the indices of erythrocytopoiesis in service dogs for physical activity. The studies were conducted on 1.5−2 years old Belgian Shepherd (Malinois) dogs whoperformed exercises from the international Mondioring program, which included physical andemotional training to train dogs for the Armed Forces, Border Troops and National Police. Blood was collected from animals before training (at rest) and after the training process (lasting 2 hours). It was found that 62.5% of animals showed a tendency to increase the total number of erythrocytes and their populations −"old" and "mature". Such changes in dogs during exercise are indicative of more intense maturation of “young” erythrocytes in the peripheral blood, elimination of “mature” cells from the depot, increased resistance to the membrane of their membranes, and intensifi cation of the processes of erythrocyte attachment and delivery. With regard to hemoglobin content in the blood and hematocrit, these fi gures in 75.0% of dogs after exercise tended to increase. However, red blood indices (MCH and MCV) tended to decrease, indicating an increase in the erythrocyte adaptation processes to physiological hypoxia during exercise and the appearance of a large number of unsaturated microcytic forms of erythrocytes in the bloodstream in particular. In the study of the ferumotransferrincomplex, it should be noted that in 87.5% of the animals, the amount of ferum, TIBC and transferrin levels increased, which are related to the release into the bloodstream of the reserve reserves of the ferrum for hemoglobin formation and adequate ability of hepatocytes to synthesize during transplantation. Key words: dogs, physical activity, erythrocytes, hemoglobin, hematocrit, acid resistance of erythrocytes, ferrum, transferrin, ferumotransferin complex.


Blood ◽  
1987 ◽  
Vol 70 (6) ◽  
pp. 1824-1829 ◽  
Author(s):  
BA Miller ◽  
O Platt ◽  
S Hope ◽  
G Dover ◽  
DG Nathan

Abstract Cytotoxic drugs increase circulating fetal hemoglobin levels. We examined the mechanism by measuring the fetal hemoglobin produced per BFU-E-derived erythroblast following hydroxyurea treatment in vivo and in vitro. Treatment of four sickle cell patients increased the percentage of circulating F reticulocytes. The frequencies of bone marrow or peripheral blood BFU-E or CFU-E-derived colonies and their fetal hemoglobin content were unaffected. In all cases, the number of erythroid cells/progenitor-derived colony increased. To explore further the effect of hydroxyurea on fetal hemoglobin production, we added 50 mumol/L hydroxyurea to cultures of peripheral blood BFU-E-derived erythroblasts on 1 of 9 days (day 5 through 13) to nine samples. These BFU-E were derived from the peripheral blood of normal donors, sickle trait donors, and sickle cell anemia patients and from the bone marrows of monkeys. This concentration of hydroxyurea was selected so that the frequency of BFU-E and their size was moderately decreased. Addition of hydroxyurea to these progenitor-derived erythroid cells had no effect on fetal hemoglobin content per cell. Neither did transient exposure of progenitors to hydroxyurea prior to culture in nontoxic concentrations (0 to 500 mumol/L) result in a significant increase in fetal hemoglobin content in progenitor-derived erythroblasts. These data suggest that hydroxyurea does not directly alter the HbF program expressed by progenitor-derived erythroid cells. Instead, it enhances hemoglobin F content secondarily, possibly by inducing alterations in erythropoiesis.


1969 ◽  
Vol 26 (11) ◽  
pp. 2881-2886 ◽  
Author(s):  
Anne Chlebeck ◽  
Gary L. Phillips

A hematological study was made based on 10 specimens each of the buffalofishes Ictiobus cyprinellus and I. bubalus (Catostomidae). Average results of analyses of selected blood characteristics of I. cyprinellus and I. bubalus, respectively, were: hemoglobin content, 7.8 and 8.5 g/100 ml; erythrocyte count, 1.08 and 1.28 millions/mm2; hematocrit, 34.6 and 32.9%; mean corpuscular hemoglobin, 67 and 64 μμg; and leucocyte count, 23916 and 29004 cells/mm3. Types of leucocytes noted were lymphocytes, neutrophils, "secretory granulocytes," eosinophils, and thrombocytes. No basophils were found. The secretory granulocyte is apparently derived from the neutrophil series. The status of the secretory granulocyte in buffalofishes is discussed with reference to pertinent literature.


Blood ◽  
1987 ◽  
Vol 70 (6) ◽  
pp. 1824-1829 ◽  
Author(s):  
BA Miller ◽  
O Platt ◽  
S Hope ◽  
G Dover ◽  
DG Nathan

Cytotoxic drugs increase circulating fetal hemoglobin levels. We examined the mechanism by measuring the fetal hemoglobin produced per BFU-E-derived erythroblast following hydroxyurea treatment in vivo and in vitro. Treatment of four sickle cell patients increased the percentage of circulating F reticulocytes. The frequencies of bone marrow or peripheral blood BFU-E or CFU-E-derived colonies and their fetal hemoglobin content were unaffected. In all cases, the number of erythroid cells/progenitor-derived colony increased. To explore further the effect of hydroxyurea on fetal hemoglobin production, we added 50 mumol/L hydroxyurea to cultures of peripheral blood BFU-E-derived erythroblasts on 1 of 9 days (day 5 through 13) to nine samples. These BFU-E were derived from the peripheral blood of normal donors, sickle trait donors, and sickle cell anemia patients and from the bone marrows of monkeys. This concentration of hydroxyurea was selected so that the frequency of BFU-E and their size was moderately decreased. Addition of hydroxyurea to these progenitor-derived erythroid cells had no effect on fetal hemoglobin content per cell. Neither did transient exposure of progenitors to hydroxyurea prior to culture in nontoxic concentrations (0 to 500 mumol/L) result in a significant increase in fetal hemoglobin content in progenitor-derived erythroblasts. These data suggest that hydroxyurea does not directly alter the HbF program expressed by progenitor-derived erythroid cells. Instead, it enhances hemoglobin F content secondarily, possibly by inducing alterations in erythropoiesis.


Copeia ◽  
1976 ◽  
Vol 1976 (1) ◽  
pp. 186 ◽  
Author(s):  
Gustav A. Engbretson ◽  
Victor H. Hutchison

1930 ◽  
Vol 52 (1) ◽  
pp. 23-38 ◽  
Author(s):  
Louise Pearce ◽  
Albert E. Casey

Observations are reported on the consecutive weekly erythrocyte counts and the hemoglobin contents of the peripheral blood in 5 groups of normal rabbits, comprising 45 animals, during a period of 20 months from October, 1927 to July, 1929. The duration of individual group examinations varied from 8 to 35 weeks. The results are analyzed on the basis of the weekly mean values of each group. On the whole, the erythrocyte values were quite uniform within a narrow range of variation, while the hemoglobin content was comparatively irregular within a wider range of variation. The major changes in the levels of mean values of both the red cells and the hemoglobin, however, were found to be statistically significant. The directions or trends in the levels of the erythrocyte and hemoglobin mean values did not necessarily move in opposite directions. The general levels of the erythrocyte and hemoglobin mean values were not identical for two consecutive years, those of 1927–28 being higher than those of 1928–29. The fluctuations of both red cell and hemoglobin mean values observed in one group of animals were also usually observed in another group examined during the same months.


1989 ◽  
Vol 5 (3) ◽  
pp. 399-404 ◽  
Author(s):  
Helena Miszta

The effect of lead on the stromal cells of bone marrow in rats was studied in cultures in vitro. Toxic effects of lead were manifested by a decrease in erythrocyte count and hemoglobin level in peripheral blood. Lead stimulated an increase in the number of fibroblasts, while the macrophage count dropped. The number of adipocytes remained unaffected.


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