Veterinary and sanitary characteristics of quail meat by feeding nanocrystalline cerium dioxide

The unresolved problem of quail is feed stress and high sensitivity of poultry of this species to feed quality. To eliminate such consequences it is possible with the use of various biologically active feed additives. However, their introduction into the diet automatically raises questions about the quality and safety of the products for the consumer. The purpose of the research is to conduct a veterinary and sanitary assessment of the quality of quail meat for feeding NDC. The purpose of the research is to conduct a veterinary and sanitary assessment of the quality of quail meat for feeding NDC. The object of study were the quails of the Pharaoh breed, at the age of 24 were divided into two groups: experimental and control 30 heads each. The birds were kept in cages with free access to food and water. Quails of the experimental group were added to drinking water feed additive "Nanocerium" at a dose of 8.6 mg per liter of drinking water. Nanocerium feed additive is an aqueous dispersion of NDC with average nanoparticle sizes of 2–7 nm. Antemortem examination of quails of both groups revealed a satisfactory clinical condition of birds. The average weight of the gut carcass of quails of the experimental group was higher by 8.3% compared to the control. Examination of 20 carcasses of quails showed that they can be classified as first class. According to organoleptic parameters, quail meat is of good quality in veterinary and sanitary terms. Microbiological studies of quail meat indicate no effect of NDC in the applied doses on its bacterial contamination. Chemical parameters of meat (pH, aminoammonia nitrogen, volatile fatty acids during storage in the refrigerator) (5 days, t = 4–5 ° C) tended to increase and were within the normal range for fresh product. Tissue in quails of both groups was not detected. The biological value of quail meat in both groups was identical and no toxicity. Tasting evaluation of the broth and meat showed that NDC did not affect the studied taste. The obtained results show that the quails that received the feed additive "Nanocerium" with water according to the indicators of veterinary and sanitary examination are of good quality, which allows to use it in human food without restrictions. Key words: poultry, nanocerium, organoleptic evaluation, microbial contamination, biological value, tasting evaluation.

Dissemination and clinical and hematological manifestations in cat cestodes

The materials of the presented research cover the issues of the spread of cestodes of cats in the Solomyansky district of Kyiv (according to the veterinary clinic Vet Alliance). After analyzing the relevant scientific literature, the epizootic situation of dipilidiosis and mesocestoidosis of cats in the metropolis was analyzed. Studies have shown that more than 50 % of parasitic diseases in cats occur in associated forms, among which the most common are toxocariasis, dipilidiosis, mesocestoidosis, ktenocephalidosis (flea infestation) and otodectosis. The monoform of invasions occurred only in 14.7 % of registered cases. The spread of diplidiosis and mesocestoidosis was characterized by seasonality – the peak of the lesion cats were observed in September-October. The most susceptible to infection Dipylidium caninum and Mesocestoides lineatus were kittens under the age of six months. The clinical condition and hematological parameters of kittens with dipilidiosis were also studied. Depression, anemia of the conjunctiva and mucous membranes of the nose and mouth, indigestion, dry cough were found. All cats had dull fur, and numerous dark brown spots and live fleas were observed on the skin, especially in the lumbar region. These data indicate a violation of erythropoiesis in feline dipilidiosis, which was characterized by erythrocytopenia and leukocytosis. Significant eosinophilia, an increase in the number of rod-shaped, and a decrease in segmental neutrophils were also found compared with clinically healthy animals. The results of biochemical blood tests of sick cats were important to clarify the features of the pathogenesis of dipilidiosis. Changes in blood biochemical parameters were characterized by a probably reduced content of hemoglobin, total protein and albumin. This indicates not only a violation of erythropoiesis, but also a violation of the protein-synthesizing function of the liver, because the pathological process involves various organs and systems of the body. Key words: feline dipilidiosis, feline mesocestoidosis, prevalence, clinical condition, hematological parameters, pulse rate, mucosal anemia, indigestion.

Influence of the prebiotic Bio-active on natural resistance, safety and productivity of young pigs

The primary task of the livestock industry is to provide the population with high-quality food products, and the industry with raw materials. For this, it is necessary to ensure a high level of productivity and safety of young pigs. Studies carried out in recent years indicate an increase in the incidence of gastrointestinal diseases among young farm animals, leading to a decrease in the immunobiological reactivity of the piglets' organism and significant damage to farms. When performing the research, we used zootechnical, zoohygienic, biochemical, immunological and variational-statistical research methods. Research work was carried out during 2010-2018 at the Department of Animal Hygiene and Fundamentals of Veterinary Medicine of the Bila Tserkva National Agrarian University. Scientific and economic experiments and production tests were carried out in the farms of Progress LLC (Uzin, Bila Tserkva district) and Denisenko LLC (Skvirsky district, Kyiv region). Experimental studies were carried out in the Problem Laboratory of Immunology, Department of Animal Hygiene and Fundamentals of Veterinary Medicine, interfaculty laboratory of biochemical and histochemical research methods. For the first time, the prebiotic Bio-active was used as a feed additive in the diet of young pigs on growing at industrial keeping, its positive effect on the safety, productivity, physiological and immunological state of their body was proved. For the first time, the optimal dose of the prebiotic Bio-active for growing pigs was established and a method of its use was developed. The optimal dose of the prebiotic Bio-active for growing young pigs is 5 g (5x107 CFU) per 10 kg of body weight when fed with compound feed once a day for 30 days, which contributes to an increase in the average daily weight gain of piglets by 17.2 % (P <0.01) and 16.6 % (P <0.05) on the 30th and 60th days of the study, respectively. Feeding pigs with Bio-active promotes an increase in the content of total blood serum protein by 7.2 % (P <0.05), albumin – by 5.2 %, γ-globulins – by 6.3 %, an increase in AST activity – by 23, 1 % (P <0.05) and ALT – by 22.4 % (P <0.01). In the peripheral blood of rearing pigs under the influence of the prebiotic Bio-active, the proliferation, differentiation and specialization of immunocompetent cells increase: an increase in the total number of T-lymphocytes by 5.43 % (P <0.05), B-lymphocytes by 2.85 %, a decrease in the content of 0-lymphocytes – by 8.29 % (P <0.05). The number of medium-avid T-lymphocytes also increases – by 9.19 % (P <0.05) and B-lymphocytes – by 10.28 % (P <0.05) due to a decrease in the level of low-avid immunocompetent cells. The use of the prebiotic Bio-active in an optimal dose promotes the activation of metabolic processes, antigen-nonspecific immunity and an increase in the body weight of pigs. Key words: industrial pig breeding, rearing young pigs, housing conditions, gastrointestinal canal, prebiotic, immunobiological reactivity, metabolism, safety.

Epizootic process of demodicosis and sarcoptosis of pet dogs in the metropolis

Scabies mites, in particular Sarcoptes scabei and Demodex canis, remain undesirable but permanent members of zoocenoses in metropolis around the world. This is due to the contacts of domestic animals with wild relatives (foxes, raccoon dogs, coyotes), which are massively infected with various types of ticks. The purpose of our research was to find out the epizootic situation with demodicosis and sarcoptic mange of domestic dogs in Kyiv, as well as to establish the sources of their infection. The actual results were obtained on the basis of the "Veterinary Clinic of Doctor Medvedev" (Kyiv) according to the data of routine work with patients for 2009-2020. Diagnostic procedures consisted of clinical and dermatological examination of dogs, examination of skin scrapings, trichoscopy, "Scotch tape" test, a Wood's lamp examination and others. Over 11 years, 1473 dermatological examinations of domestic dogs were carried out. Of these, 230 dogs (15.6%) suffered from demodicosis at the age of 5 months to 7 years. Sarcoptic mange was much less common: a total of 59 sick dogs (4.0%) were identified. Both of acaroses were seasonal, with an increase in the incidence in fall and winter. Regarding the breed factor: Pugs (10.43%), Staffordshire Terriers (10.0%) and French Bulldogs (9.56%) were more prone to demodicosis; to sarcoptic mange – German wirehaired pointer (13.6%), German hunt terrier (11.9%) and Shorthaired pointer (10.2%). The stray dogs and cats were source most often of infection of domestic decorative dogs with mites Sarcoptes scabei var canis. Hunting dogs mainly became infected during the hunting season during contact with animals of the wild fauna, most often – foxes. Clinical manifestations of acaroses occurred mainly in young animals not older than 3 years (70.5 and 76.7% for demodicosis and sarcoptic mange, respectively). In addition, in male dogs sarcoptic mange is more often recorded (59.3%), in female dogs – demodicosis (53.9%). Key words: scabies, mites, Sarcoptes scabiei, Demodex canis, prevalence, dogs, foxes.

Modern condition of the problem of Lime Burreliosis of animals (systematic review)

Lyme borreliosis (LB) is the most common tick-borne disease in the temperate climate of the Northern Hemisphere. LB is caused by spirochetes, which are grouped in the complex Borrelia burgdorferi sensu lato, vectors – Ixodidae mites, dozens of species of small mammals and birds actas reservoir hosts, and the role of reservoirs of domestic animals, such as dogs and cats, is not excluded. Although the ecology and epidemiology of the disease have been extensively studied in Europe and North America, there is considerable uncertainty regarding the study of Lyme borreliosis in veterinary medicine. Search, selection andanalysis of scientific data on the research topic were carried out according to acceptable rules for systematic reviews of the literature. The scientometric database Web of Science Core Collection, the database of scientific articles Pub Med and the database Scientific Periodicals of Ukraine were used. Seventy-four scientific articles were used for the article, which contained the necessary set of data and met the set goal. The article highlights the main issues of the etiology of the disease, which describes the characteristics of the pathogen, its properties, genotypic composition of Borrelia, which cause Lyme disease, the spread of pathogenic genotypes of Borrelia in Europe and Ukraine. The connection of Lyme borreliosis spread with ecological factors, climatic changes and anthropogenic impact on biocenoses and biotopes, the role of vectors and reservoir hosts in the spread of LB is described. Data on the prevalence and degree of infection of Ixodes mites with Borrelia, as well as the spread of LB among the population of Europe and Ukraine are presented. The seroprevalence of each of the animal species (dogs, cats, horses, ruminants), clinical manifestations of LB, if documented and indicators of LB prevalence among animals in Europe and Ukraine, as well as currently known treatments, prevention and diagnosis of LB animals are considered. Key words: Borrelia Burgdorferi Sensu Lato, Lyme Diseases Etiology, Ixodidae.

Prospects for the creation and use of paired and multiple correlation and regression models in beekeeping

In animal husbandry, including beekeeping, there are a growing number of independent consultancy services to analyse the performance of the industry in relation to disease monitoring status and preventive measures to maintain proper bee family health. In order to provide expert advice, these services must always be backed up by quality data and accurate statistical analysis. It would give clear instructions on how to interpret the results obtained when processing them, and show directions for improving disease prevention. Currently, there are problems related to improving the control of infectious diseases in bees, as various natural and anthropogenic factors have a multidirectional effect on the economic performance of beekeeping. There are also concerns about the control of infectious animal and insect diseases, which is a multifaceted series of causes due to natural and anthropogenic factors that have a polyvector effect on the economic performance of beekeeping. Therefore, the experimental application of different types of correlation and regression analysis in this industry by constructing pairwise and multivariate dependencies and their statistical interpretation was the aim of the paper. The correlation and regression model under study contains four sets of characteristics: result variable (y) - the amount of honey from 20 different apiaries in one season and factor variables: x1 - air temperature in the apiaries; x2 - amount of probiotic "Enteronormin Iodis + Se" to stimulate the immune system as one of the preventive methods; x3 - number of beehives in each apiary. Linear proportional relationships between apiary productivity and the factors included in the regression model are obtained. According to the results of the correlation-regression analysis, paired correlation coefficients showed that the relationship between air temperature in the apiary and produced honey is medium connection (r1 = 0,666), the relationship between the amount of probiotic applied per frame and produced honey is tight (close) connection (r2 = 0,813), the relationship between the number of beehives and produced honey is medium connection (r3 = 0,633). The regression coefficients show how the amount of honey produced in an apiary changes when each factor changes by one, with the other factors in the equation fixed. So, raising the temperature by 1 °C increases the honey production by 216 kg in each apiary, while increasing the concentration of "Enteronormin Iodis + Se" by 1 cm3 per beehive frame increases the nectar production by 1,12 kg for one hive. The coefficient of multiple determination (R2 = 0,954163) identifies a close relationship in the model created (95% of the factors investigated determine apiary performance). Therefore, modelling in the form of linear and multiple correlation and regression analysis is feasible in beekeeping. Key words: beekeeping, modeling, system analysis, factor and result characteristics.

Coagulase-positive staphylococci in dogs and their antimicrobial resistance (systematic review)

Staphylococcus spp. a common genus of bacteria that colonizes the body of dogs. Some families of this genus are pathogenic and are involved in the development of diseases of various systems and organs, especially the skin and outer ear. One of the main signs of the most pathogenic families of this genus is the secretion of the enzyme coagulase. They are grouped into the group of coagulase-positive Staphylococcus (CoPS). S. pseudintermedius most often is isolated from a dog, about 10-60% isolates. S. aureus is identified less frequently, in 4-15% of cases, but it is associated with people who are its natural carriers. S. schleiferi subsp. coagulans is another family that can be identified in the pathological material obtained from dogs. However, coagulase is one of much different pathogenicity factors in this family. Also, microorganisms of this family can exist both in the form of a planktonic culture and in the structure of a biofilm, on which the effect of antibacterial agents is weakened. Some strain of CoPS carry genes of resistance to various antibacterial agents and may occupy a dominant position in the pathogenic process. Such strains can colonize the environment in places where animals congregate, which leads, for example, to the occurrence of clinical infections. Also resistant are strains of this family that can colonize other animals or humans. Owners and specialists working with animals are constantly at risk. To date, the issue of the emergence of resistance to beta-lactam antibiotics (methicelin-resistant strains) and the acquisition of resistance to several groups of antibacterial agents is acute. In the countries of the European Union, a fairly large number of studies are conducted aimed at studying the spread of Staphylococcus spp. analysis of virulence and pathogenicity factors, as well as mechanisms of acquiring antibacterial resistance. Programs to control the prevalence of resistant strains are also being developed there. Different authors show resistance to a large number of antibacterial agents, the profile of antibacterial resistance may differ both within different groups and between different drugs of the same pharmaceutical group. In Ukraine, there are no systematic studies of the spread of resistant strains of microorganisms among small animals. Key words: Staphylococcus spp., S. pseudintermedius, S. aureus, S. schleiferi subsp. Coagulans, CoPS, MRSP, MRSA, antibacterial resistance, dogs.

Dynamics of biochemical bone and endothelial parameters during the replacement of bone defects in dogs with hydroxyapatite ceramic doped with silicon

Metal structures for osteosynthesis available in veterinary orthopedics are not able to compensate for the lost elements of bone tissue in complex splinter fractures. It is prompt the use of hydroxyappatite materials that replaced bone defects for maintenance of osteoconductive function, and ideally would combine osteointegration and osteoinductive properties. However, their influence on the biological processes of fracture consolidation which go through a number of successive stages and end with the formation of bone tissue in the fracture zone identical to the maternal, is insufficiently substantiated according to the criteria of the molecular biological phase of reparative osteogenesis. The aim of the study was to investigate the dynamics of biochemical osteotropic parameters and the level of NO using silicon-doped ceramics for fractures heeling in dogs. Materials and methods. The animals suffering of fractures that were admitted to the faculty clinic were divided into control (n=7) and experimental (n=7) groups. In both groups, extracortical osteosynthesis was performed with a support plate from an unalloyed titanium alloy. In the control group, bone defects were left to heal under spontaneous blood clot, and in the experimental group, they were replaced with ceramic based on hydroxyapatite with β-tricalciumphosphate doped with silicon (HA/β-TCP/l-Si–3).Blood samples were taken after the injury no later than the 48th day, and on the 3th, 12th, 21th, 42th and 60th days after osteosynthesis. To increase the objectivity of the biochemical analysis, we additionally formed a group of clinically healthy dogs that were admitted to the clinic for routine vaccination (n=10). It included the spectrophotometric determination of the content of NO, BALP, TRACP, Ca, P, Mg, total protein in blood serum, and fibrinogen in blood plasma. Research results. A clinical study showed that in the case of using HA/β-TCP/l-Si–3for splinter fractures, the stages of reparative osteogenesis are more optimized in time, and their consolidation occurs on average 19 days earlier than in the control group. The results of the biochemical study showed that when using HA/β-TCP/l-Si–3, it is accompanied by a peak NO value already on the third day, which is significantly higher than in the control group and indicates early angiogenesis in the research group. In terms of TRACP, the period of osteoresorption in the control group was permanent with little expressed peaks of activity. However, in the research group, the peak of TRACP activity is limited to 12 and 21 days, which is evidence of an optimized inflammatoryresorptive phase. In parallel with this, the activity of BALP increases, which indicates the consistency of the stages of reparative osteogenesis and provides an optimized and accelerated consolidation of fractures in the research group. Conclusion. The dynamics of NO, BALP and TRACP pathochemically substantiates the optimized reparative osteogenesis when using HA/β-TCP/l-Si–3 for bone defects replacement in cases of splinter fractures of tubular bones. Key words: bone markers, bone isoenzyme of alkaline phosphatase, NO, tartrate-resistant acid phosphatase, fibrinogen, calcium, phosphorus.

Conducting a comprehensive mycological study to prevent the occurrence of aspergillosis in chickens

Diseases of poultry aspergillosis are recorded in many poultry farms, the causes of the disease are the violation of the sanitary and hygienic conditions of hatcheries and poultry houses (unfavorable microclimate, affected feed and litter by microscopic fungi). To prevent the development of infection, it is necessary to control air pollution and equipment in hatcheries; determine the safety indicators of feed and water (the level of their mycological contamination) in poultry houses; monitor the status of feed storage facilities and water supply systems. According to the results of our research, in 15.1% of dead chickens at the age of 3 to 5 days and 6.6% at the age of 7-10 days, according to the results of mycological examination of pathological material, the diagnosis of aspergillosis was established. Aspergillus flavus (47%) Aspergillus fumigatus (34.5%), Aspergillus niger (10%) were isolated from the lungs of dead chickens. Macroscopically revealed edema and hyperemia of the lungs in chickens of 3-5 days of age. The formation of granulomas in the lungs and serous membranes was not detected, which may indicate an acute form of the pathological process. Chickens 7-10 days old showed a strong thickening of the air sacs, lungs with multiple spherical granulomas up to 2 mm in diameter. Spores of fungi entering the lungs cause a local inflammatory reaction - acute pneumonia. Histologically, in chickens of 3-5 days of age, a thickening of the parabronchial wall due to hyperplasia of local lymphoid formations (providing local immunity), signs of the development of interstitial pneumonia were revealed. Pathognomonic signs were found in the parenchyma - the formation of giant multinucleated cells, which is a characteristic sign of mycotic infections in poultry. The fusion of local pulmonary macrophages, histiocytes, into multinucleated cells is a prerequisite for the formation of granulomas, being a specific immune response in birds to the penetration of a pathogen. According to the development of the infectious process, caseous granulomas and fungal hyphae can be detected microscopically in the lungs. According to the results of histological examination, the formation of mycotic granulomas was found in the lung parenchyma in 7-10 days old chickens, which was accompanied by the formation of a zone of coagulation necrosis, in which the fungal hyphae were found. On the periphery, giant multinucleated epithelioid cells are located, the detection of which by histological examination is the basis for suspecting bird infection with microscopic fungi. Microscopic fungi Aspergillus flavus and Aspergillus fumigatus have been found in washes from hatchery ventilation cabinets, and Aspergillus flavus was found in the air from the chick sorting room and in washes from the feed mixer. Key words: aspergillosis, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, micromycete, mold fungi, histiocytes, granulomas, phylaids, conidia, hyphae.

Analysis of changes in hematological indexes and essential trace elements metabolism under the using of vitamin-amino acid complex

It has been established that the level of blood parameters in the body of sheep is not always stable and often depends on the breed, sex, their physiological state, the level of productivity, feeding and housing conditions. Among the factors that determine the optimal sheep nutritional level, not the last place is occupied by their provision with a sufficient amount of essential amino acids, especially lysine, methionine, cystine, and vitamins, given their physiological importance for the animal body. The overwhelming amount of them is able to be synthesized in the sheep rumen with the help of microorganisms of the gastrointestinal tract, however, some of them are irreplaceable, that is, those whose balance must be replenished through feed rations. The article describes the use of the vitamin-amino acid complex "Аlphabet for Animals" in sheep, containing a complex of fat-and water-soluble vitamins, as well as amino acids, which contribute to the normalization of metabolism in the body of small ruminants, increase its resistance, have a positive effect on productivity, safety and reproductive animals functions. During the research, the effect of this feed additive on hemocytopoiesis and the exchange of essential micronutrients in ewes were studied. After a double use of the additive, an increase in the processes of erythrocytopoiesis was established, as evidenced by an increase in the number of blood erythrocytes by 21.2% and the hemoglobin content by 11.2%, stabilization of the size and degree of saturation of erythrocytes with hemoglobin, as evidenced by a decrease in MCH and MCV indices. There were no significant changes in the content of essential micronutrients in the blood serum of sheep under the influence of the "Alphabet for Animals", and a slight increase of zinc content in the blood was noted in sheep after the second injection of the drug. At the same time, in the blood of experimental animals group a significant increase the iron content on average up to 129.5±7.33 μg/100 ml (p<0.05) was established, and the number of animals with a low amount of blood iron was only 12.5 %. The number of leukocytes in the sheep blood of both groups was within the normal range and during the second and third blood tests did not change significantly. Key words: sheep, feeding, feed additives, amino acids, vitamins, hemocytopoiesis, essential micronutrients.