Determination of background serum alpha-fetoprotein level in blood donors by combined electrophoresis and precipitation in polyacrylamide gel

SummaryA one-stage lysis time system containing fibrinogen, streptokinase, thrombin, and a known, small amount of plasminogen was used to determine plasminogen in plasma.The known amount of plasminogen was added to the system in order to keep the lysis times relatively short when a highly diluted plasma was tested. High dilutions of plasma were used to reduce the influence of the plasma inhibitors.The calculation of the plasminogen concentration was made on the basis of the correlation: “plasminogen = fibrinogen/lysis time” which was valid in the system. The method allowed determination of plasminogen in plasma with varying fibrinogen concentrations, as the fibrinogen concentration in plasma was considered in the calculation.The presence of “spontaneous” plasmin activity in the plasma did not influence the plasminogen determination. Estimated by this method, the plasminogen content in plasma from 32 blood donors aged 25-45 years was 13.1 ±2.4 casein u/ml. The error of a single determination was 0.3 casein u/ml. The plasminogen content in plasma, determined with the present method, is about 3-4 times higher than the content found when a caseinolytic method is used.

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Faculty Opinions recommendation of Defining hepatoblastoma responsiveness to induction therapy as measured by tumor volume and serum alpha-fetoprotein kinetics.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.5078969.5016077 ◽

2010 ◽

Author(s):

Piotr Czauderna

Keyword(s):

Induction Therapy ◽

Tumor Volume ◽

Alpha Fetoprotein ◽

Serum Alpha Fetoprotein

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RESULTS OF 12 YEARS' COMBINED MATERNAL SERUM ALPHA-FETOPROTEIN SCREENING AND ULTRASOUND FETAL MONITORING FOR PRENATAL DETECTION OF FETAL MALFORMATIONS IN HAVANA CITY, CUBA

Prenatal Diagnosis ◽

10.1002/(sici)1097-0223(199704)17:4<301::aid-pd969>3.0.co;2-x ◽

1997 ◽

Vol 17 (4) ◽

pp. 301-304 ◽

Cited By ~ 7

Author(s):

L. RODRÍGUEZ ◽

R. SÁNCHEZ ◽

J. HERNÁNDEZ ◽

L. CARRILLO ◽

J. OLIVA ◽

...

Keyword(s):

Fetal Monitoring ◽

Maternal Serum ◽

Alpha Fetoprotein ◽

Prenatal Detection ◽

Fetal Malformations ◽

Serum Alpha Fetoprotein ◽

Havana City

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Evaluation of serum alpha fetoprotein-L3 as an accuracy novel biomarker for the early diagnosis of hepatocellular carcinoma in Egyptian Patients

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2021.06.020 ◽

2021 ◽

Author(s):

Hassnaa Mohamed Ibrahim ◽

Magdy Zaki Elghannam ◽

Om Ali Yousef Elkhawaga ◽

Ahmed Mohamed Adel El-Sokkary

Keyword(s):

Hepatocellular Carcinoma ◽

Early Diagnosis ◽

Alpha Fetoprotein ◽

Egyptian Patients ◽

Serum Alpha Fetoprotein

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Hepatoid adenocarcinoma of the lung and the review of the literature

Journal of Oncology Pharmacy Practice ◽

10.1177/1078155220903360 ◽

2020 ◽

Vol 26 (6) ◽

pp. 1505-1510

Author(s):

Onder Tonyali ◽

Onur Gonullu ◽

Mehmet Akif Ozturk ◽

Aysun Kosif ◽

Ozlenen Gonca Civi

Keyword(s):

Concurrent Chemoradiotherapy ◽

Mediastinal Lymph Node ◽

Alpha Fetoprotein ◽

Disease Stage ◽

Hepatoid Adenocarcinoma ◽

Rare Type ◽

Adenocarcinoma Of The Lung ◽

Female Smoker ◽

Diagnostic Biopsy ◽

Serum Alpha Fetoprotein

Introduction Hepatoid adenocarcinoma of the lung is an extremely rare type of the non-small cell lung cancer. Treatment principles and prognosis are similar to that of lung adenocarcinoma. Case report A 62-year-old female smoker presented with a huge mass in the left upper lobe. After diagnostic biopsy, she underwent left pneumonectomy and mediastinal lymph node dissection. A diagnosis of stage T4N1M0 hepatoid adenocarcinoma of the lung with positive surgical margins was made. Management and outcome After the operation, the level of serum alpha fetoprotein was 9010 ng/ml (N: <10). The level of serum alpha fetoprotein was decreased with concurrent chemoradiotherapy and chemotherapy. Disease progression was detected at 11 months after diagnosis. No response was obtained to other therapies. The patient died at 14 months from the time of diagnosis. Discussion Usually, patients with hepatoid adenocarcinoma of the lung are male smokers. Hepatoid adenocarcinoma tends to settle in the upper lobes of the lung. The most important prognostic factor of the hepatoid adenocarcinoma of the lung is the disease stage at the diagnosis and patients with metastatic disease have poor survival.

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