Effect of burn trauma on reactivity of mouse spleen cells of different genotypes in the regional graft versus host reaction

1990 ◽  
Vol 109 (6) ◽  
pp. 793-795
Author(s):  
A. N. Barsukov ◽  
G. N. Fedorov ◽  
V. F. Semenkov
Keyword(s):  
Mouse Spleen ◽  
Graft Versus Host Reaction ◽  
Spleen Cells ◽  
Host Reaction ◽  
Burn Trauma ◽  
Graft Versus Host

Biopterin Synthesis in Mouse Spleen during Bone Marrow Transplantation Correlates with Unimpaired Hemopoietic Engraftment

1987 ◽  
Vol 42 (4) ◽  
pp. 461-464 ◽  
Author(s):  
Irmgard Ziegler ◽  
Stefan Thierfelder
Keyword(s):  
Monoclonal Antibody ◽  
Bone Marrow ◽  
Donor Cell ◽  
Mouse Spleen ◽  
Graft Versus Host Reaction ◽  
Allogeneic Bone ◽  
Bone Marrow Transplants ◽  
Host Reaction ◽  
Graft Versus Host ◽  
Biopterin Synthesis

Abstract Normal engraftment of bone marrow transplants depends on histocompatibility between donor and recipient. In this case reconstitution of hemopoiesis and lymphopoiesis in the mouse spleen is correlated with transient biopterin synthesis in these cells. Allogeneic bone marrow progeny cells undergo donor cell proliferation in the spleen prior to the incidence of graft-versus-host reaction. These cells are not committed to biopterin synthesis. Thy-1 monoclonal antibody fully repairs engraftment of allogeneic transplants together with biopterin synthesis.

scholarly journals GRAFT-VERSUS-HOST REACTION ACROSS DIFFERENT REGIONS OF THE H-2 COMPLEX OF THE MOUSE

1973 ◽  
Vol 137 (5) ◽  
pp. 1213-1225 ◽  
Author(s):  
Jan Klein ◽  
Jong M. Park
Keyword(s):  
T Cells ◽  
Mixed Lymphocyte Reaction ◽  
Graft Versus Host Reaction ◽  
Spleen Cells ◽  
Host Reaction ◽  
Graft Versus Host ◽  
D Region ◽  
The Individual ◽  
Ir Genes

H-2 crossovers and their parental strains were arranged into 35 combinations in which the adult donor of spleen cells differed from the newborn recipient in the whole H-2 complex, or in three, two, or one region of the complex. A Simonsen splenomegaly assay was then used to test the contribution of the individual H-2 regions to the graft-versus-host reaction (GVHR). It was shown that the strongest GVHR was associated with the Ir region. Differences in the Ir region caused significant splenomegaly in spite of the fact that no antigens detectable by conventional serological methods have thus far been associated with this region. Differences in the K and D regions showed only a borderline effect on GVHR in spite of the fact that these regions code for most, if not all, of the antigens detectable by conventional serological and transplantation methods. The K region alone caused no stronger GVHR than the D legion alone; however, K + Ir region differences led to much stronger GVHR than D region differences. The Ss-Slp region also showed only a borderline effect on GVHR. Differences in two or more H-2 regions usually caused greater splenomegaly than differences in each of the regions separately. On the basis of these findings it is concluded that the strongest GVHR is caused by genes distinct from the known histocompatibility genes of the H-2 complex. It is speculated that the GVHR genes are identical with the mixed lymphocyte reaction (MLR) and Ir genes and that the product of these genes are receptors on the surface of the thymus-derived lymphocytes (T cells).

Effect of cis-Platinum (II) Diamminedichloride and Gallium Nitrate on the Ability of Spleen Cells to Induce a Graft-Versus-Host Reaction

1974 ◽  
Vol 146 (2) ◽  
pp. 333-336 ◽  
Author(s):  
A. M. Munster ◽  
P. Greenberg ◽  
S. Greenberg ◽  
A. G. Leary ◽  
G. R. Gale
Keyword(s):  
Graft Versus Host Reaction ◽  
Gallium Nitrate ◽  
Spleen Cells ◽  
Host Reaction ◽  
Graft Versus Host

FATAL RESPONSE SUGGESTIVE OF GRAFT-VERSUS-HOST REACTION FOLLOWING TRANSPLANTATION OF SPLEEN CELLS FROM ALLOGENEIC ATHYMIC (NUDE) DONORS

1981 ◽  
Vol 31 (3) ◽  
pp. 201-204 ◽  
Author(s):  
JAMES P. OKUNEWICK ◽  
RUBY F. MEREDITH ◽  
RADMILA B. RAIKOW ◽  
BARBARA J. BROZOVICH ◽  
KATHLEEN MAGLIERE
Keyword(s):  
Graft Versus Host Reaction ◽  
Spleen Cells ◽  
Host Reaction ◽  
Graft Versus Host

Selective effects of graft-versus-host reaction on disaccharidase expression by mouse jejunal enterocytes

1986 ◽  
Vol 71 (2) ◽  
pp. 189-198 ◽  
Author(s):  
E. K. Lund ◽  
M. G. Bruce ◽  
M. W. Smith ◽  
A. Ferguson
Keyword(s):  
Graft Versus Host Reaction ◽  
Lactase Activity ◽  
Spleen Cells ◽  
Host Reaction ◽  
Graft Versus Host ◽  
Neonatal Mice ◽  
Crypt Hyperplasia ◽  
Biochemical Analyses ◽  
Precocious Development ◽  
Quantitative Cytochemistry

1. Graft-versus-host reaction (GvHR) was induced in neonatal mice to produce crypt hyperplasia with and without stunted villi. Lactase activity was measured along individual villi of control and GvHR mice using quantitative cytochemistry. 2. Lactase activity increased in control mice as enterocytes migrated over the lower part of the villus. This increase was followed by a period when lactase activity remained approximately constant. 3. Effects produced by GvHR on this normal profile of development included an extension of the distance on the villus over which enterocytes could continue to increase lactase activity, a reduction in the time needed for an enterocyte to express lactase activity at maximal rate, and an overall decrease in the maximal lactase activity expressed by mature enterocytes. 4. These effects have been quantified by fitting logistic curves to the experimental data. 5. Parallel biochemical analyses of intestinal homogenates showed sucrase, isomaltase, trehalase and maltase activities to increase markedly 7–8 days after the injection of parental spleen cells. 6. Attention is drawn to similarities between these results and steroid induced precocious development of intestinal function in neonatal mice.

Modulation of Cell-Mediated Immunity by Lithium Chloride

1994 ◽  
Vol 49 (9-10) ◽  
pp. 679-683 ◽  
Author(s):  
M. Kubera ◽  
M. Bubak-Satora ◽  
V. Holan ◽  
W. Krol ◽  
A. Basta-Kaim ◽  
...  
Keyword(s):  
Lithium Chloride ◽  
Nk Cell ◽  
Graft Versus Host Reaction ◽  
Cell Mediated Immunity ◽  
Spleen Cells ◽  
Host Reaction ◽  
Graft Versus Host ◽  
Control Value ◽  
Licl Treatment

Abstract Immunomodulation of cell-mediated immunity was studied in mice treated with either lithium chloride (LiCl), anti-CD 8 monoclonal antibody or their combination. While 6-day LiCl treatment decreased the ability of their splenocytes to induce a local graft-versus-host reaction -anti-CD 8 abolished this effect. The proliferative response of spleen cells from those three groups of mice to concanavalin A stimulation in vitro was significantly increased. The natural killer (NK) cell toxicity of the mice was decreased by over 43% after the 6-day LiCl treatment, but was ×2.5 higher then the control value after a longer 21-d treatment. These results indicate that the immunomodulatory capacity of lithium is dependent on the type of cell population studied, and on the schedule of administration.

scholarly journals THE INTERACTION OF DONOR AND HOST LYMPHOID CELLS IN THE PATHOGENESIS OF RENAL CORTICAL DESTRUCTION INDUCED BY A LOCAL GRAFT VERSUS HOST REACTION

1966 ◽  
Vol 123 (1) ◽  
pp. 103-118 ◽  
Author(s):  
William L. Elkins
Keyword(s):  
Host Cells ◽  
Whole Body ◽  
Graft Versus Host Reaction ◽  
Developmental Phase ◽  
Donor Strain ◽  
Spleen Cells ◽  
Host Reaction ◽  
F1 Hybrid ◽  
Graft Versus Host ◽  
Donor Type

The graft versus host reaction (GVHR), which results from the injection of parental strain spleen cells beneath the kidney capsule of F1 hybrid rats, is transferable during its developmental phase into F1 hybrid hosts isogeneic with the primary host, but not into secondary hosts of the parental (donor) strain. Furthermore, the GVHR propagates but rarely in secondary hybrid hosts which are allogeneic with respect to the primary hosts, but which are also genetically tolerant of donor-type cells. These findings indicate that the donor cells not only initiate the GVHR but also maintain it by virtue of immunologically specific activity. Whole-body irradiation of (LBf)F1 and (LBN)F1 hosts 24 hours prior to the injection of parental (L) spleen cells results in inhibition of the subsequent GVHR to a degree commensurate with the radiation damage sustained by the lymphoid system of the host. Furthermore, propagation of transferred GVHRs did not occur if susceptible secondary hybrid hosts had been previously irradiated. These findings indicate that radiosensitive host cells play a continuing and essential role in the pathogenesis of the invasive-destructive lesion. It is concluded that the development of this lesion depends upon the continuous interaction of the specifically reactive donor-type cells with an immunologically non-specific population of host mononuclears.

scholarly journals Autoantibodies in chronic graft versus host result from cognate T-B interactions.

1990 ◽  
Vol 171 (2) ◽  
pp. 503-517 ◽  
Author(s):  
S C Morris ◽  
R L Cheek ◽  
P L Cohen ◽  
R A Eisenberg
Keyword(s):  
T Cells ◽  
B Cells ◽  
Direct Interaction ◽  
Graft Versus Host Reaction ◽  
Spleen Cells ◽  
Host Reaction ◽  
Graft Versus Host ◽  
Alloreactive T Cells ◽  
Donor T Cells ◽  
And Control

A chronic graft-versus-host reaction (GVH) induced in nonautoimmune mice causes a syndrome that closely resembles SLE. In this model, donor T cells react against incompatible host Ia structures and generate excessive help, which activates a subpopulation of self-reactive B cells. We have studied whether these self-reactive B cells are activated by direct interaction with alloreactive T cells or by nonspecific bystander effects. Two types of chimeras were made: double-parental chimeras, differing at both Ia and Igh allotype [B6.C20 + bm12----(B6.C20 x bm12)F1]; and control chimeras [(B6.C20 x bm12)F1----(B6.C20 x bm12)F1]. A chronic GVH syndrome was induced in the chimeras by infusion of B6 or bm12 spleen cells. Coombs and antichromatin autoantibodies were measured using Igh allotype-specific immunoassays. The double-parental chimeras that received bm12 cells made autoantibodies principally of the Igha allotype, indicating that the bm12 T cells interacted only with the Iab-bearing host B cells. Conversely, double-parental chimeras that received B6 cells made mostly Ighb autoantibodies, indicating direct cognate interaction with the Iabm12-bearing host B cells. The control chimeras made autoantibodies of both allotypes. These results indicate that autoantibodies in chronic GVH result from direct T-B interactions and not from nonspecific T cell-derived factors.

Effect of the graft-versus-host reaction on the immunological responsiveness of the mouse

1961 ◽  
Vol 154 (957) ◽  
pp. 532-539 ◽  
Keyword(s):  
Graft Versus Host Reaction ◽  
Bacterial Antigen ◽  
Spleen Cells ◽  
Host Reaction ◽  
Graft Versus Host ◽  
Loss Of Weight ◽  
Immunological Reactions ◽  
First Time ◽  
Hybrid Mice ◽  
Special Case

Graft-versus-host reaction has been induced in unirradiated adult ( C 57 BL x CBA ) F 1 hybrid mice by intravenous injection of parent-line spleen cells, and the capacity of the injected animals to react to first-set and second-set grafts of A -line skin, and to Salm. typhi H antigen, has been investigated. Injection of CBA cells resulted in little or no loss of weight, a low mortality, little or no impairment of the recipient’s capacity to react to A skin or to the bacterial antigen when encountered for the first time, and no loss of pre-existing immunity to A skin. Injection of (40 to 100) x 10 6 C 57 BL cells on the other hand resulted in severe loss of weight and diarrhoea, often culminating in death. Surviving non-immunized animals reacted feebly to the bacterial antigen and some were slower than normal in rejecting first-set grafts of A skin. Pre-existing immunity to A skin was lost except in the special case when the spleen cell donor had itself been immunized against A skin. It is concluded that severe graft-versus-host reaction depresses the host’s immunological reactivity, but that the foreign cells may enable the host to muster a variety of immunological reactions by proxy.

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