Biomass composition and methods for the determination of metabolic reserve polymers in phototrophic sulfur bacteria

1994 ◽  
Vol 56 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Claudio Del Don ◽  
Kurt W. Hanselmann ◽  
Raffaele Peduzzi ◽  
Reinhard Bachofen
Keyword(s):  
Biomass Composition ◽  
Phototrophic Sulfur Bacteria ◽  
Sulfur Bacteria ◽  
Metabolic Reserve

scholarly journals Anoxygenic photo- and chemosynthesis of phototrophic sulfur bacteria from an alpine meromictic lake

2021 ◽  
Author(s):  
Francesco Di Nezio ◽  
Clarisse Beney ◽  
Samuele Roman ◽  
Francesco Danza ◽  
Antoine Buetti-Dinh ◽  
...  
Keyword(s):  
Carbon Fixation ◽  
Meromictic Lake ◽  
Purple Sulfur Bacterium ◽  
Phototrophic Sulfur Bacteria ◽  
Anaerobic Microorganisms ◽  
Sulfur Bacteria ◽  
Pure Cultures ◽  
Chlorobium Phaeobacteroides ◽  
Anoxic Environment ◽  
Dark Carbon Fixation

Abstract Meromictic lakes are interesting ecosystems to study anaerobic microorganisms due their permanent stratification allowing the formation of a stable anoxic environment. The crenogenic meromictic Lake Cadagno harbors an important community of anoxygenic phototrophic sulfur bacteria responsible for almost half of its total productivity. Besides their ability to fix CO2 through photosynthesis, these microorganisms also showed high rates of dark carbon fixation via chemosyntesis. Here, we grew in pure cultures three populations of anoxygenic phototrophic sulfur bacteria previously isolated from the lake, accounting for 72.8% of the total microbial community, and exibiting different phenotypes: 1) the motile, large-celled purple sulfur bacterium (PSB) Chromatium okenii, 2) the small-celled PSB Thiodictyon syntrophicum, and 3) the green sulfur bacterium (GSB) Chlorobium phaeobacteroides. We measured their ability to fix CO2 through photo- and chemo-synthesis, both in situ in the lake and in laboratory under different incubation conditions. We also evaluated the efficiency and velocity of H2S photo-oxidation, an important reaction in the anoxygenic photosynthesis process. Our results confirm that phototrophic sulfur bacteria strongly fix CO2 in the presence of light and that oxygen increases chemosynthesis at night, in laboratory conditions. Moreover, substancial differences were displayed between the three selected populations in terms of activity and abundance.

Computational determination of the pigment binding motif in the chlorosome protein a of green sulfur bacteria

2013 ◽  
Vol 118 (3) ◽  
pp. 231-247 ◽  
Author(s):  
Sándor Á. Kovács ◽  
William P. Bricker ◽  
Dariusz M. Niedzwiedzki ◽  
Peter F. Colletti ◽  
Cynthia S. Lo
Keyword(s):  
Protein A ◽  
Green Sulfur Bacteria ◽  
Binding Motif ◽  
Sulfur Bacteria ◽  
Green Sulfur

Vertical gradient of nutrients in two dimictic lakes ? influence of phototrophic sulfur bacteria on nutrient balance

2004 ◽  
Vol 66 (3) ◽  
Author(s):  
Uwe Selig ◽  
Thomas H�bener ◽  
Reinhard Heerkloss ◽  
Hendrik Schubert
Keyword(s):  
Nutrient Balance ◽  
Vertical Gradient ◽  
Phototrophic Sulfur Bacteria ◽  
Sulfur Bacteria

Determination of CHO biomass composition

2018 ◽  
Vol 44 ◽  
pp. S144-S145
Author(s):  
D. Széliová ◽  
D. Ruckerbauer ◽  
S.N. Galleguillos ◽  
M. Hanscho ◽  
N. Borth
Keyword(s):  
Biomass Composition

scholarly journals Determination of Hemicellulose, Cellulose, and Lignin Content in Different Types of Biomasses by Thermogravimetric Analysis and Pseudocomponent Kinetic Model (TGA-PKM Method)

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1048
Author(s):  
David Díez ◽  
Ana Urueña ◽  
Raúl Piñero ◽  
Aitor Barrio ◽  
Tarja Tamminen
Keyword(s):  
Thermogravimetric Analysis ◽  
Kinetic Model ◽  
Lignin Content ◽  
Low Cost ◽  
Biomass Composition ◽  
Fast Method ◽  
Additional Information ◽  
Extraction And Purification ◽  
Different Types

The standard method for determining the biomass composition, in terms of main lignocellulosic fraction (hemicellulose, cellulose and lignin) contents, is by chemical method; however, it is a slow and expensive methodology, which requires complex techniques and the use of multiple chemical reagents. The main objective of this article is to provide a new efficient, low-cost and fast method for the determination of the main lignocellulosic fraction contents of different types of biomasses from agricultural by-products to softwoods and hardwoods. The method is based on applying deconvolution techniques on the derivative thermogravimetric (DTG) pyrolysis curves obtained by thermogravimetric analysis (TGA) through a kinetic approach based on a pseudocomponent kinetic model (PKM). As a result, the new method (TGA-PKM) provides additional information regarding the ease of carrying out their degradation in comparison with other biomasses. The results obtained show a good agreement between experimental data from analytical procedures and the TGA-PKM method (±7%). This indicates that the TGA-PKM method can be used to have a good estimation of the content of the main lignocellulosic fractions without the need to carry out complex extraction and purification chemical treatments. In addition, the good quality of the fit obtained between the model and experimental DTG curves (R2Adj = 0.99) allows to obtain the characteristic kinetic parameters of each fraction.

scholarly journals A Benthic Gradient Chamber for culturing phototrophic sulfur bacteria on reconstituted sediments

1996 ◽  
Vol 20 (4) ◽  
pp. 237-250 ◽  
Author(s):  
Olivier Pringault ◽  
Rutger Wit ◽  
Pierre Caumette
Keyword(s):  
Phototrophic Sulfur Bacteria ◽  
Sulfur Bacteria

scholarly journals Dynamic cellular complexity of anoxygenic phototrophic sulfur bacteria in the chemocline of meromictic Lake Cadagno

PLoS ONE ◽  
2017 ◽  
Vol 12 (12) ◽  
pp. e0189510 ◽  
Author(s):  
Francesco Danza ◽  
Nicola Storelli ◽  
Samuele Roman ◽  
Samuel Lüdin ◽  
Mauro Tonolla
Keyword(s):  
Meromictic Lake ◽  
Phototrophic Sulfur Bacteria ◽  
Sulfur Bacteria

scholarly journals Community Shift from Phototrophic to Chemotrophic Sulfide Oxidation following Anoxic Holomixis in a Stratified Seawater Lake

2014 ◽  
Vol 81 (1) ◽  
pp. 298-308 ◽  
Author(s):  
Petra Pjevac ◽  
Marino Korlević ◽  
Jasmine S. Berg ◽  
Elvira Bura-Nakić ◽  
Irena Ciglenečki ◽  
...  
Keyword(s):  
Water Column ◽  
Sulfide Oxidation ◽  
Water Layer ◽  
Tight Coupling ◽  
Phototrophic Sulfur Bacteria ◽  
Community Shift ◽  
Sulfur Bacteria ◽  
Microbiological Methods ◽  
Culture Independent ◽  
The Impact

ABSTRACTMost stratified sulfidic holomictic lakes become oxygenated after annual turnover. In contrast, Lake Rogoznica, on the eastern Adriatic coast, has been observed to undergo a period of water column anoxia after water layer mixing and establishment of holomictic conditions. Although Lake Rogoznica's chemistry and hydrography have been studied extensively, it is unclear how the microbial communities typically inhabiting the oxic epilimnion and a sulfidic hypolimnion respond to such a drastic shift in redox conditions. We investigated the impact of anoxic holomixis on microbial diversity and microbially mediated sulfur cycling in Lake Rogoznica with an array of culture-independent microbiological methods. Our data suggest a tight coupling between the lake's chemistry and occurring microorganisms. During stratification, anoxygenic phototrophic sulfur bacteria were dominant at the chemocline and in the hypolimnion. After an anoxic mixing event, the anoxygenic phototrophic sulfur bacteria entirely disappeared, and the homogeneous, anoxic water column was dominated by a bloom of gammaproteobacterial sulfur oxidizers related to the GSO/SUP05 clade. This study is the first report of a community shift from phototrophic to chemotrophic sulfide oxidizers as a response to anoxic holomictic conditions in a seasonally stratified seawater lake.

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