Organotypic culture of dissociated fetal rat lung cells on a collagen sponge matrix

1978 ◽  
Vol 4 (1) ◽  
pp. 749-753 ◽  
Author(s):  
William H. J. Douglas ◽  
James A. McAteer ◽  
Thomas Cavanagh
Keyword(s):  
Organotypic Culture ◽  
Collagen Sponge ◽  
Lung Cells ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Sponge Matrix

An Ultrastructural Comparison of Rat Lung Cells Grown in an Organotypic Culture System with Lung Tissue Found in the Whole Animal

1976 ◽  
Vol 34 ◽  
pp. 172-173
Author(s):  
Peter J. Del Vecchio ◽  
Edward P. Dougherty ◽  
William H. J. Douglas
Keyword(s):  
Lung Tissue ◽  
Organotypic Culture ◽  
Fetal Lung ◽  
Lung Cells ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Tissue Organization ◽  
Fetal Rat ◽  
Fetal Rat Lung

This study describes the ultrastructure of an organotypic system prepared from rat fetal lung and compares it to lung in rat fetuses. The preparation of the organotypic system has been described elsewhere (1).The organotypic systems in this study are prepared from 18-19 day fetal rat lung and spend two days in culture making the cells a total of 20-21 days old. The fine structure of the type II cells present in this system will be compared to the type II cells in fetal lung at 20-21 days gestation. Because the tissue organization of the organotypic system is more like the organization of fetal lung at an earlier stage (18-19 days gestation) , the cell to cell relationships of the organotypic system are compared to fetal lung tissue at this stage.The histology of the organotypic system is similar to that of the fetal lung in its glandular stage (Fig. 1). The epithelial cells are all columnar and are surrounded by relatively undifferentiated mesenchyme.

The formation of histotypic structures from monodisperse fetal rat lung cells cultured on a three-dimensional substrate

In Vitro ◽  
1976 ◽  
Vol 12 (5) ◽  
pp. 373-381 ◽  
Author(s):  
William H. J. Douglas ◽  
Gary W. Moorman ◽  
Robert W. Teel
Keyword(s):  
Three Dimensional ◽  
Lung Cells ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Cells Cultured

scholarly journals Differential Regulation of Glucocorticoid Receptor Expression by Ligand in Fetal Rat Lung Cells

1995 ◽  
Vol 38 (4) ◽  
pp. 506-512 ◽  
Author(s):  
Neil Sweezey ◽  
Carolyn Mawdsley ◽  
Felicia Ghibu ◽  
Li Song ◽  
Shilpa Buch ◽  
...  
Keyword(s):  
Glucocorticoid Receptor ◽  
Differential Regulation ◽  
Receptor Expression ◽  
Lung Cells ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung

Mechanical stretch stimulates macrophage inflammatory protein-2 secretion from fetal rat lung cells

2000 ◽  
Vol 279 (4) ◽  
pp. L699-L706 ◽  
Author(s):  
Eric Mourgeon ◽  
Noritaka Isowa ◽  
Shaf Keshavjee ◽  
Xiaoming Zhang ◽  
Arthur S. Slutsky ◽  
...  
Keyword(s):  
Mechanical Stretch ◽  
Lung Cells ◽  
Maximal Effect ◽  
Mrna Levels ◽  
Rat Lung ◽  
Inflammatory Protein ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Inhibitor Of Protein Synthesis ◽  
Macrophage Inflammatory Protein

Ventilation-induced lung injury has been related to cytokine production. Immaturity and barotrauma are important contributors to the development of bronchopulmonary dysplasia in infants. In the present study, stretch of organotypic cultured fetal rat lung cells was used to simulate ventilation of preterm newborns. Cells were stimulated with lipopolysaccharide (LPS; 100 ng/ml) and/or mechanical stretch. After 4 h, stretch enhanced LPS-induced macrophage inflammatory protein (MIP)-2 production in a force- and frequency-dependent manner. The maximal effect of stretch was seen with 5% elongation at 40 cycles/min. In contrast, after 1 h of stimulation, stretch alone significantly increased MIP-2 production, which was not blocked by cycloheximide, an inhibitor of protein synthesis. At both the 1- and 4-h time points, only LPS increased MIP-2 mRNA levels. Stretch-induced MIP-2 release was associated with cell injury as measured by lactate dehydrogenase release and was not inhibited by gadolinium, a stretch-activated ion channel blocker. Taken together, these results suggest that the major effect of stretch on MIP-2 production from fetal rat lung cells is to stimulate its secretion.

scholarly journals Mechanical strain-induced posttranscriptional regulation of fibronectin production in fetal lung cells

1999 ◽  
Vol 277 (1) ◽  
pp. L142-L149 ◽  
Author(s):  
Eric Mourgeon ◽  
Jing Xu ◽  
A. Keith Tanswell ◽  
Mingyao Liu ◽  
Martin Post
Keyword(s):  
Cell Proliferation ◽  
Mechanical Strain ◽  
Fetal Lung ◽  
Northern Analysis ◽  
Normal Lung ◽  
Lung Cells ◽  
Mrna Levels ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung

We have shown that intermittent mechanical strain, simulating fetal breathing movements, stimulated fetal rat lung cell proliferation. Because normal lung growth requires proper coordination between cell proliferation and extracellular matrix remodeling, we investigated the effect of strain on fibronectin metabolism. Organotypic cultures of fetal rat lung cells, subjected to intermittent strain, showed increased fibronectin content in the culture media. Fibronectin-degrading activity in media from strained cells was similar to that of static cultures. Northern analysis revealed that strain inhibited fibronectin mRNA accumulation seen during static culture. Synthesis of fibronectin, determined by metabolic labeling, was increased by strain despite lower mRNA levels or presence of actinomycin D. This increase was not mediated via a rapamycin-sensitive mechanism. Strain stimulated prelabeled fibronectin secretion even in the presence of cycloheximide. These results suggest that strain differentially regulates fibronectin production of fetal lung cells at the transcriptional and posttranscriptional levels. Mechanical strain increases soluble fibronectin content by stimulating its synthesis and secretion without increasing fibronectin message levels.

scholarly journals Mechanical Strain Induces pp60 Activation and Translocation to Cytoskeleton in Fetal Rat Lung Cells

1996 ◽  
Vol 271 (12) ◽  
pp. 7066-7071 ◽  
Author(s):  
Mingyao Liu ◽  
Yi Qin ◽  
Jason Liu ◽  
A. Keith Tanswell ◽  
Martin Post
Keyword(s):  
Mechanical Strain ◽  
Lung Cells ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung

Differential regulation of extracellular matrix molecules by mechanical strain of fetal lung cells

1999 ◽  
Vol 276 (5) ◽  
pp. L728-L735 ◽  
Author(s):  
Jing Xu ◽  
Mingyao Liu ◽  
Martin Post
Keyword(s):  
Extracellular Matrix ◽  
Cell Proliferation ◽  
Mechanical Strain ◽  
Fetal Lung ◽  
Lung Cells ◽  
Gelatinase A ◽  
Rat Lung ◽  
Fetal Rat ◽  
Gene And Protein Expression ◽  
Fetal Rat Lung

We have previously shown that an intermittent mechanical strain regimen (5% elongation, 60 cycles/min, 15 min/h) that simulates fetal breathing movements stimulated fetal rat lung cell proliferation. Because normal lung growth requires proper coordination between cell proliferation and extracellular matrix (ECM) remodeling, we subjected organotypic cultures of fetal rat lung cells ( day 19 of gestation, term = 22 days) to this strain regimen and examined alterations in ECM gene and protein expression. Northern analysis revealed that mechanical strain reduced messages for procollagen-α1(I) and biglycan and increased the levels of mRNA for collagen-α1(IV) and -α2(IV), whereas laminin β-chain mRNA levels remained constant. Regardless of mRNA changes, mechanical strain increased the protein content of type I and type IV collagen as well as of biglycan in the medium. Mechanical strain did not affect gene expression of several matrix metalloproteinases (MMPs), such as MMP-1 (interstitial collagenase), MMP-2 (gelatinase A), and MMP-3 (stromelysin-1). Neither collagenase nor gelatinase (A and B) activities in conditioned medium were affected by mechanical strain. Tissue inhibitor of metalloproteinase activities in conditioned medium remained unchanged during the 48-h intermittent mechanical stretching. These data suggest that an intermittent mechanical strain differentially regulates gene and protein expression of ECM molecules in fetal lung cells. The observed increase in matrix accumulation appears to be mainly a result of an increased synthesis of ECM molecules and not of decreasing activity of degradative enzymes.

Insulin-like Growth Factor Binding Protein Production and Regulation in Fetal Rat Lung Cells

1993 ◽  
Vol 8 (4) ◽  
pp. 425-432 ◽  
Author(s):  
Wayne A. Price ◽  
Billie M. Moats-Staats ◽  
A. Joseph D'Ercole ◽  
Alan D. Stiles
Keyword(s):  
Growth Factor ◽  
Protein Production ◽  
Binding Protein ◽  
Lung Cells ◽  
Insulin Like Growth Factor ◽  
Rat Lung ◽  
Factor Binding ◽  
Fetal Rat ◽  
Fetal Rat Lung
Sign in / Sign up

Export Citation Format

Share Document