Preganglionic parasympathetic innervation in normal and partially denervated rat stomach

1991 ◽  
Vol 23 (2) ◽  
pp. 181-186
Author(s):  
L. P. Voitenko ◽  
B. S. Polinkevich ◽  
T. V. Beregovaya ◽  
L. Ya. Shtanova ◽  
S. D. Groisman
Keyword(s):  
Rat Stomach ◽  
Parasympathetic Innervation

Electron-Microscopic localization of biotinylated gastrin bound to eosinophils in the rat stomach

1994 ◽  
Vol 52 ◽  
pp. 300-301
Author(s):  
Caroline A. Miller ◽  
David H. Nichols ◽  
Richard F. Murphy
Keyword(s):  
Electron Microscope ◽  
Phosphate Buffer ◽  
Uranyl Acetate ◽  
List Type ◽  
Cerium Chloride ◽  
Rat Stomach ◽  
Electron Microscopic ◽  
Human Sequence ◽  
Acetate Lead ◽  
Microscope Slides

Gastrin is a small peptide capable of both stimulating gastric acid secretion and acting as an enteric growth factor. Known functions of eosinophils in the rat stomach are related to immunological defense. Here we demonstrate the binding of biotinylated gastrin to rat stomach eosinophils in the electron microscope. Small pieces of stomach were fixed by immersion in 4% paraformaldehyde/0.1% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4 for 1 hour. The tissue was then cryoprotected in 30% sucrose/0.1 M phosphate buffer, transferred to Tissue Tek OCT compound and frozen in isopentane cooled with liquid nitrogen. Transverse cryostat sections were cut at 25 μm, thawed in PBS and free floating sections exposed to 10−5 M biotinylated 1-17 gastrin (human sequence; Peninsula Labs) for 1 hour. Controls omitted the biotinylated gastrin from this step. Sections were then rinsed 3X in PBS and exposed to either:1).a 1:50 dilution of 10 nm Extravidin colloidal gold (Sigma) for 2 hours, or2).an avidin-biotin-alkaline phosphatase complex (ABC-AP;Vector) for 1 hour. A substrate solution containing cerium chloride was used to generate an electron dense reaction product.Sections from both procedures were postfixed in 1% OsO4 in 0.1 M phosphate buffer, rinsed and dehydrated. These were then flat embedded in EMbed 812 between two microscope slides coated with Liquid Release (both from Electron Microscopy Sciences).Polymerized sections were adhered to resin blocks using super glue, cut at 70-90 nm, stained with uranyl acetate/lead citrate and observed in a Philips CM-10 electron microscope.

Effect of Interleukin-8 on histamine release from isolated rat stomach

2001 ◽  
Vol 120 (5) ◽  
pp. A159-A159
Author(s):  
S RO ◽  
K YAKABI ◽  
T NAKAMURA
Keyword(s):  
Histamine Release ◽  
Interleukin 8 ◽  
Rat Stomach ◽  
Isolated Rat

Effect of galanin on gastrin release from the isolated rat stomach

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S31
Author(s):  
TH. SEUFFERLEIN ◽  
V. SCHUSDZIARRA ◽  
S. MADAUS ◽  
M. CLASSEN
Keyword(s):  
Gastrin Release ◽  
Rat Stomach ◽  
Isolated Rat

Modulatory glucose effect on bombesin-like immunoreactivity and gastrin secretion from isolated perfused rat stomach

Diabetes ◽  
1986 ◽  
Vol 35 (7) ◽  
pp. 791-796 ◽  
Author(s):  
V. Schusdziarra ◽  
R. Schmid ◽  
M. Classen
Keyword(s):  
Rat Stomach ◽  
Glucose Effect ◽  
Gastrin Secretion

Effects of bilateral nephrectomy on endocrine cells of rat stomach

1978 ◽  
Vol 8 (4) ◽  
pp. 401-401
Author(s):  
H. A. Munshid ◽  
F. Sundler ◽  
R. Håkanson
Keyword(s):  
Endocrine Cells ◽  
Bilateral Nephrectomy ◽  
Rat Stomach

scholarly journals The α-Synuclein Origin and Connectome Model (SOC Model) of Parkinson’s Disease: Explaining Motor Asymmetry, Non-Motor Phenotypes, and Cognitive Decline

2021 ◽  
Vol 11 (2) ◽  
pp. 455-474
Author(s):  
Per Borghammer
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Cognitive Decline ◽  
Explanatory Power ◽  
Anatomical Location ◽  
Asymmetric Distribution ◽  
Motor Asymmetry ◽  
Parasympathetic Innervation ◽  
Considerable Body ◽  
Dopaminergic Degeneration

A new model of Parkinson’s disease (PD) pathogenesis is proposed, the α-Synuclein Origin site and Connectome (SOC) model, incorporating two aspects of α-synuclein pathobiology that impact the disease course for each patient: the anatomical location of the initial α-synuclein inclusion, and α-synuclein propagation dependent on the ipsilateral connections that dominate connectivity of the human brain. In some patients, initial α-synuclein pathology occurs within the CNS, leading to a brain-first subtype of PD. In others, pathology begins in the peripheral autonomic nervous system, leading to a body-first subtype. In brain-first cases, it is proposed that the first pathology appears unilaterally, often in the amygdala. If α-synuclein propagation depends on connection strength, a unilateral focus of pathology will disseminate more to the ipsilateral hemisphere. Thus, α-synuclein spreads mainly to ipsilateral structures including the substantia nigra. The asymmetric distribution of pathology leads to asymmetric dopaminergic degeneration and motor asymmetry. In body-first cases, the α-synuclein pathology ascends via the vagus to both the left and right dorsal motor nuclei of the vagus owing to the overlapping parasympathetic innervation of the gut. Consequently, the initial α-synuclein pathology inside the CNS is more symmetric, which promotes more symmetric propagation in the brainstem, leading to more symmetric dopaminergic degeneration and less motor asymmetry. At diagnosis, body-first patients already have a larger, more symmetric burden of α-synuclein pathology, which in turn promotes faster disease progression and accelerated cognitive decline. The SOC model is supported by a considerable body of existing evidence and may have improved explanatory power.

Immunohistochemical relationships of huntingtin-associated protein 1 with enteroendocrine cells in the pyloric mucosa of the rat stomach

2020 ◽  
Vol 122 (8) ◽  
pp. 151650
Author(s):  
Akie Yanai ◽  
Md Nabiul Islam ◽  
Maki Hayashi-Okada ◽  
Mir Rubayet Jahan ◽  
Abu Md Mamun Tarif ◽  
...  
Keyword(s):  
Enteroendocrine Cells ◽  
Rat Stomach ◽  
Pyloric Mucosa

scholarly journals Pharmacological activities of a kinin released from rat plasma by catheptic enzyme in rat stomach.

1983 ◽  
Vol 33 (6) ◽  
pp. 1119-1126
Author(s):  
Motoki KOBAYASHI ◽  
Katsuya OHATA
Keyword(s):  
Rat Plasma ◽  
Rat Stomach ◽  
Pharmacological Activities

Cellular localization and distribution of neurokinin-1 receptors in the rat stomach

2003 ◽  
Vol 104 (2) ◽  
pp. 95-108 ◽  
Author(s):  
Linda Yip ◽  
Yin Nam Kwok ◽  
Alison M.J. Buchan
Keyword(s):  
Cellular Localization ◽  
Rat Stomach ◽  
Neurokinin 1
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