Ameliorative activity of aqueous leaf extract from Madhuca longifolia against diclofenac-administered toxicity on rat stomach and intestine

Diclofenac is used to treat rheumatism disorders, which are associated with the damages of renal, gastric and hepatic organs. Diclofenac is a pharmaceutical drug that is known to induce toxicity on its overdosage and long-term usage. Madhuca longifolia is known to have antioxidant, anti-inflammatory and anti-ulcer activity. It is an evergreen tree that is reported to have many ethnomedicinal uses. The other properties of Madhuca longifolia include anti-diabetic, analgesic and anti-microbial activities. Our study aims to evaluate the pre-treatment activity against the diclofenac-induced toxicity by the Madhuca longifolia aqueous leaf extract in Wistar albino rats for 10 and 15 days. Rats were divided as Group-I: Normal control, Group-II: Diclofenac on the last two days, Group-III and group-IIIa: Diclofenac + Aqueous Leaf Extract of Madhuca longifolia, Group-IV and group-IVb: Diclofenac + Silymarin, Group -V and group-Va: Aqueous Madhuca longifolia leaf extract. After the sacrifice, the rats were studied for antioxidant assay, renal enzyme markers, liver enzyme markers, and histopathological analysis of the kidney, stomach, intestine, and liver. As a result, we could identify that Madhuca longifolia has reduced the toxic changes in rats caused by diclofenac.

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Simultaneous Determination of Gallic Acid and Quercetin in Leaf Extract of Madhuca longifolia from Myanmar by HPLC-DAD: Method Development and Validation

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.859.51 ◽

2020 ◽

Vol 859 ◽

pp. 51-56

Author(s):

Aye Thida ◽

Onoomar Toyama ◽

Malai Satiraphan

Keyword(s):

Gallic Acid ◽

Leaf Extract ◽

Method Development ◽

Research Work ◽

Hplc Method ◽

Madhuca Longifolia ◽

Method Development And Validation ◽

Aqueous Leaf Extract ◽

Development And Validation

Madhuca longifolia (J.Koenig ex L.) Macbr. belongs to the Sapotaceae family. This research work focused on the determination of gallic acid and quercetin in an aqueous leaf extract of M. longilolia. The development and validation of the analytical method using HPLC-DAD have been performed. The validated method was applied to determine both compounds in the leaves of M. longifolia. Optimized HPLC conditions were developed and validated for specificity, linearity, sensitivity, precision and accuracy. The linearity ranges and linear regression value were 3.125 to 100 μg/mL with r2=1 for gallic acid and 0.78 to 50 μg/mL with r2=0.9999 for quercetin, respectively. The LOD and LOQ were 0.24 and 0.73 μg/mL for gallic acid and 0.21 and 0.63 μg/mL for quercetin. Intra and inter-day precision were in the range of 0.18 to 1.76. The recovery percentage were 103.86% to 104.98% for gallic acid and 100.10% to 102.97% for quercetin. The concentration ranges of gallic acid and quercetin in the extracts were 207.95 to 405.79 mg/100 g and 7.31 to 20.56 mg/100 g, respectively. The developed HPLC method was considered to be accurate, precise and reliable for the determination of gallic acid and quercetin in M. longifolia aqueous leaf extract. This may be the first-time report for HPLC-DAD method development for the determination of bioactive phenolic compounds in Madhuca longifolia from Myanmar.

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Cytoprotective and antiadhesive effects of aqueous leaf extract from Orthosiphon aristatus against uropathogenic E. coli

Planta Medica ◽

10.1055/s-0036-1596551 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381 ◽

Cited By ~ 1

Author(s):

S Sarshar ◽

MR Asadi Karam ◽

M Habibi ◽

S Bouzari ◽

X Qin ◽

...

Keyword(s):

Leaf Extract ◽

E Coli ◽

Aqueous Leaf Extract

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Insecticidal Activities of the Leaf Extract of the Invasive Alien Plant, Chromolaena odorata (L.) (Asteraceae) Against Macrotermes species (Termites)

Nigeria Journal of Entomology ◽

10.36108/nje/9102/53.01.31 ◽

2019 ◽

Vol 35 (1) ◽

pp. 145-153

Author(s):

O. Uyi, ◽

I.G. Amolo ◽

A.D. Adetimehin

Keyword(s):

Leaf Extract ◽

Insect Pests ◽

Chromolaena Odorata ◽

Leaf Extracts ◽

Alien Plant ◽

Biological Efficacy ◽

Invasive Alien Plant ◽

Impregnation Technique ◽

Aqueous Leaf Extract ◽

Worker Caste

Several studies have demonstrated the biological efficacy of leaf, stem and root powders or extracts of Chromolaena odorata (L.) King and Robinson against insect pests but those that are focused on the biological efficacy of aqueous leaf extracts against Macrotermes species are scanty. Current management of termites with synthetic insecticides is being discouraged due to human and environmental hazards. Therefore, the insecticidal effectiveness of aqueous leaf extract C. odorata against Macrotermes species was investigated. Five concentrations (0, 2.5, 5.0, 7.5 and 10.0% (w/v)) of the aqueous extract of C. odorata plant were evaluated for repellency and toxicity on the worker caste of Macrotermes species following standard procedures. The filter paper impregnation technique was used for the bioassay. Percentage repellency was monitored for 30 minutes and mortality recorded at 12, 24 and 36 hours post exposure. The leaf extract of C. odorata significantly repelled 95% of Macrotermes species at the highest concentration of 10% (w/v) after 30 minutes post treatment exposure. Mortality of Macrotermes species was independent of treatment concentration, but dependent on duration of exposure. All treatment concentrations of aqueous leaf extract of C. odorata caused significant mortality against Macrotermes species ranging between 94% and 98% compared to the control; indicating very great potential for adoption and use in the management of Macrotermes species.

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Green Synthesis of Silver Nanoparticles by Allamanda cathartica L. Leaf Extract and Evaluation for Antimicrobial Activity

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2013.6.4.9 ◽

2013 ◽

Vol 6 (4) ◽

pp. 2260-2268

Author(s):

M. Linga Rao ◽

Bhumi G ◽

Savithramma N

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Leaf Extract ◽

Fusarium Species ◽

Inhibition Zone ◽

Fungal Species ◽

Green Technology ◽

Metallic Silver ◽

Synthesis Of Nanoparticles ◽

Aqueous Leaf Extract

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent. The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals. In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica. The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM). The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size. These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species. These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.

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