Characterization of ferrioxamine E as the principal siderophore ofErwinia herbicola (Enterobacter agglomerans)

1988 ◽  
Vol 1 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Ingrid Berner ◽  
Silvia Konetschny-Rapp ◽  
G�nther Jung ◽  
G�nther Winkelmann
2015 ◽  
Vol 4 (1) ◽  
pp. 67 ◽  
Author(s):  
Alessandra Karisa Costa Lima do Nascimento ◽  
Lucivana Prata Souza Mourão ◽  
Enedina Nogueira de Assunção ◽  
Carlos Gustavo Nunes-Silva ◽  
Edmar Vaz de Andrade ◽  
...  

2003 ◽  
Vol 413 (1) ◽  
pp. 139-146 ◽  
Author(s):  
Adnane Moutaouakkil ◽  
Youssef Zeroual ◽  
Fatima Zohra Dzayri ◽  
Mohamed Talbi ◽  
Kangmin Lee ◽  
...  

2000 ◽  
Vol 46 (9) ◽  
pp. 856-859 ◽  
Author(s):  
Tong Li ◽  
Pierre Juteau ◽  
Réjean Beaudet ◽  
François Lépine ◽  
Richard Villemur ◽  
...  

The oxygen-sensitive 4-hydroxybenzoate decarboxylase (4OHB-DC) activity from a phenol-carboxylating coculture, consisting of Clostridium-like strain 6 and an unidentified strain 7, was studied. Assays done with cell extracts showed that the optimal pH was 5.0-6.5 and the Kmwas 5.4 mM. The activity decreased by 50% in the presence of 5 mM EDTA, and it was restored and even enhanced by the addition of Mg++, Mn++, Zn++, or Ca++. After purification, the molecular mass of the enzyme was estimated as 420 kDa by gel chromatography, and as 119 kDa by SDS-PAGE, suggesting a homotetrameric structure. Its pI was 5.6. The N-terminal amino acid sequence showed 95% and 76% homology with the pyruvate-flavodoxin oxidoreductase (nifJ gene product) from Enterobacter agglomerans and Klebsiella pneumoniae, respectively. The purified enzyme also slowly catalyzed the reverse reaction, that is the phenol carboxylation. These characteristics suggest that this enzyme is different from other known decarboxylases. This includes the 4OHB-DC from Clostridium hydroxybenzoicum, which is the only one that had been purified before.Key words: purification, 4-hydroxybenzoate decarboxylase, coculture, phenol carboxylation, anaerobic conditions.


1995 ◽  
Vol 79 (5) ◽  
pp. 496-498 ◽  
Author(s):  
Syed Muniruzzaman ◽  
Yasutaka Kunihisa ◽  
Kenji Ichiraku ◽  
Ken Izumori

2019 ◽  
Author(s):  
Anahí Oleas-Paz ◽  
Ana Cecilia Santamaría-Naranjo ◽  
Maira Rojas-Carrillo ◽  
Andrés Merino-Viteri ◽  
Alexander Genoy-Puerto

Abstract Objective: Edema syndrome is highly prevalent but under researched in captive frogs around the world. The objective of the present study was to characterize at a basic microbiological and cytological level the edema fluid of 20 individuals of the genus Gastrotheca to determine the presence of possible anaerobic and aerobic bacteria. Results: Fourteen types of bacteria were identified in the edema fluid, twelve of them at the species level ( Pasteurella haemolytica , Hafnia alvei , Enterobacter agglomerans , Aeromonas hydrophila , Pseudomonas fluorescens , Burkholderia pseudomallei , Salmonella arizonae , Enterobacter gergoviae , Enterobacter sakazakii , Yersinia enterocolitica , Klebsiella oxytoca, and Klebsiella ozaenae ) and two at the genus level ( Enterococcus spp. and Streptococcus spp.). The most frequently identified cells were lymphocytes (37.7% in females and 46.4% in males), erythrocytes (23.5% in females and 17.5% in males) and neutrophils (4.2% in females and 2.8% in males). Finally, no relationship was found between the data obtained and the sex of the individuals studied.


2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Anahí Oleas-Paz ◽  
Ana Cecilia Santamaría-Naranjo ◽  
Maira Rojas-Carrillo ◽  
Andrés Merino-Viteri ◽  
Alexander Genoy-Puerto

Abstract Objective Edema syndrome is highly prevalent but under researched in captive frogs around the world. The objective of the present study was to characterize at a basic microbiological and cytological level of the bacteria of the edema fluid of 20 individuals of the genus Gastrotheca to determine the presence of possible anaerobic and aerobic bacteria. Results Fourteen types of bacteria were identified in the edema fluid, 12 of them at the species level (Pasteurella haemolytica, Hafnia alvei, Enterobacter agglomerans, Aeromonas hydrophila, Pseudomonas fluorescens, Burkholderia pseudomallei, Salmonella arizonae, Enterobacter gergoviae, Enterobacter sakazakii, Yersinia enterocolitica, Klebsiella oxytoca, and Klebsiella ozaenae) and two at the genus level (Enterococcus spp. and Streptococcus spp.). The most frequently identified cells were lymphocytes (37.7% in females and 46.4% in males), erythrocytes (23.5% in females and 17.5% in males) and neutrophils (4.2% in females and 2.8% in males). Finally, no relationship was found between the data obtained and the sex of the individuals studied.


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