Caprine Pneumonia III. Biochemical Characterization and Serological types of pasteurellae

TWO hundred isolates of Pasteurella haemolytica and 8 isolates of P. multocida were identified by biochemical and serological methods. Among P. haemolytica strains, 6 different serotypes were found while 25 isolates were untypable. The 8 isolates of P. multocida belonged to the same serotype.

Microbiological and cytological characterization of coelomic fluid from three captive endangered amphibian Gastrotheca species with edema syndrome: preliminary analysis

Objective Edema syndrome is highly prevalent but under researched in captive frogs around the world. The objective of the present study was to characterize at a basic microbiological and cytological level of the bacteria of the edema fluid of 20 individuals of the genus Gastrotheca to determine the presence of possible anaerobic and aerobic bacteria. Results Fourteen types of bacteria were identified in the edema fluid, 12 of them at the species level (Pasteurella haemolytica, Hafnia alvei, Enterobacter agglomerans, Aeromonas hydrophila, Pseudomonas fluorescens, Burkholderia pseudomallei, Salmonella arizonae, Enterobacter gergoviae, Enterobacter sakazakii, Yersinia enterocolitica, Klebsiella oxytoca, and Klebsiella ozaenae) and two at the genus level (Enterococcus spp. and Streptococcus spp.). The most frequently identified cells were lymphocytes (37.7% in females and 46.4% in males), erythrocytes (23.5% in females and 17.5% in males) and neutrophils (4.2% in females and 2.8% in males). Finally, no relationship was found between the data obtained and the sex of the individuals studied.

Microbiological and cytological characterization of coelomic fluid from three captive endangered amphibian Gastrotheca species with edema syndrome: Preliminary analysis.

Objective: Edema syndrome is highly prevalent but under researched in captive frogs around the world. The objective of the present study was to characterize at a basic microbiological and cytological level the edema fluid of 20 individuals of the genus Gastrotheca to determine the presence of possible anaerobic and aerobic bacteria. Results: Fourteen types of bacteria were identified in the edema fluid, twelve of them at the species level ( Pasteurella haemolytica , Hafnia alvei , Enterobacter agglomerans , Aeromonas hydrophila , Pseudomonas fluorescens , Burkholderia pseudomallei , Salmonella arizonae , Enterobacter gergoviae , Enterobacter sakazakii , Yersinia enterocolitica , Klebsiella oxytoca, and Klebsiella ozaenae ) and two at the genus level ( Enterococcus spp. and Streptococcus spp.). The most frequently identified cells were lymphocytes (37.7% in females and 46.4% in males), erythrocytes (23.5% in females and 17.5% in males) and neutrophils (4.2% in females and 2.8% in males). Finally, no relationship was found between the data obtained and the sex of the individuals studied.

Channel Formation by LktA of Mannheimia (Pasteurella) haemolytica in Lipid Bilayer Membranes and Comparison of Channel Properties with Other RTX-Cytolysins

Cytolysin LktA is one of the major pathogenicity factors of Mannheimia haemolytica (formerly Pasteurella haemolytica) that is the cause of pasteurellosis, also known as shipping fever pneumonia, causing substantial loss of sheep and cattle during transport. LktA belongs to the family of RTX-toxins (Repeats in ToXins) that are produced as pathogenicity factors by a variety of Gram-negative bacteria. Sublytic concentrations of LktA cause inflammatory responses of ovine leukocytes. Higher concentrations result in formation of transmembrane channels in target cells that may cause cell lysis and apoptosis. In this study we investigated channel formation by LktA in artificial lipid bilayer membranes made of different lipids. LktA purified from culture supernatants by polyethylene glycol 4000 precipitation and lyophilization had to be activated to frequently form channels by solution in 6 M urea. The LktA channels had a single-channel conductance of about 60 pS in 0.1 M KCl, which is about one tenth of the conductance of most RTX-toxins with the exception of adenylate cyclase toxin of Bordetella pertussis. The LktA channels are highly cation-selective caused by negative net charges. The theoretical treatment of the conductance of LktA as a function of the bulk aqueous concentration allowed a rough estimate of the channel diameter, which is around 1.5 nm. The size of the LktA channel is discussed with respect to channels formed by other RTX-toxins. We present here the first investigation of LktA in a reconstituted system.

Microbiological and cytological characterization of coelomic fluid from three captive endangered amphibian Gastrotheca species with edema syndrome: Preliminary analysis.

Objective: Edema syndrome is highly prevalent but under researched in captive frogs around the world. The objective of the present study was to characterize at a basic microbiological and cytological level the edema fluid of 20 individuals of the genus Gastrotheca to determine the presence of possible anaerobic and aerobic bacteria. Results: Fourteen types of bacteria were identified in the edema fluid, twelve of them at the species level ( Pasteurella haemolytica , Hafnia alvei , Enterobacter agglomerans , Aeromonas hydrophila , Pseudomonas fluorescens , Burkholderia pseudomallei , Salmonella arizonae , Enterobacter gergoviae , Enterobacter sakazakii , Yersinia enterocolitica , Klebsiella oxytoca, and Klebsiella ozaenae ) and two at the genus level ( Enterococcus spp. and Streptococcus spp.). The most frequently identified cells were lymphocytes (37.7% in females and 46.4% in males), erythrocytes (23.5% in females and 17.5% in males) and neutrophils (4.2% in females and 2.8% in males). Finally, no relationship was found between the data obtained and the sex of the individuals studied.

Suppression of cellular immunity factors by toxic fraction of supernatant of broth culture Pasteurella haemolytica

In the etiology of many infectious animal diseases, Pasteurella haemolytica belongs to a specific place. An important factor in the pathogenicity of this microorganism, like the Pasteurella multocida serotype D, is the thermostable exotoxin. It can be obtained from bacterial-purified culture fluid. Although the study of toxin formation among microorganisms is quite popular, however, the features of toxin formation in hemolytic pasteurals, depending on the virulence of the pathogen, the nature of the toxic effects of these objects in vivo remain unclear. Materials for research were 16 isolates of Pasteurella haemolytica, isolated from pathological and biological material obtained during the outbreaks of respiratory pathology in farm animals. Initially, the nature of the research concerned the establishment of the potential for toxin formation in the isolates obtained. The method provided for a comparative analysis of the DNA nucleotide sequences of each of the investigated isolates P. haemolytica and information obtained from the international database. Another part of the work concerned the actual allocation of the major groups of toxic components of Pasteurella haemolytica by extraction to determine their biochemical nature. Exotoxin isolation was carried out from the Pasteurella spp. The components of the sediment and supernatant were separated by ion exchange chromatography on TSK gels. In order to detect the harmful effects of toxin hemolytic pasteurals on the body, they used the method of determining the opsonic index (the ratio of the phagocytic number in the mixture without the products of toxic fractions to the mixture with the toxin-containing fraction). It has been established that an important factor of the pathogenetic effect in Pasteurella haemolytica is the toxic fraction. Electrophoregram analysis of the results of DNA amplification in a comparative aspect with the data of standard samples helped to determine the presence of elements of the genome, which indicate the potential for toxin formation in isolated hemolytic pasteurized isolates from the test material. Toxic fractions isolated from Pasteurella haemolytica broth culture supernatant are substances of protein-carbohydrate nature. The isolated peak toxicogenic fractions of dialysate of a bacterial culture sieve contained protein and carbohydrates within the limits of 12.5–20 μg / ml and 0–20 μg/ml, respectively. In the dialysate of the broth culture supernatant, where 5 groups of toxigenic fractions were identified, the content of protein and carbohydrates in them varied, respectively, in the range from 20 to 95 μg/ml and from 3.3 to 26.62 μg/ml. At reproduction of opsono-phagocytic reaction with participation of toxigenic fractions of hemolytic pasteurel, a sufficiently expressed immunosuppressive effect of these complexes on the body of warm-blooded substances with an opsonic index of 3 ± 0.03 was established. During further research it is planned to determine the dermal necrotic and lethal effects of the isolated toxicogenic fractions of hemolytic pasteuride on the body of warm-blooded ones.

Pasteurella haemolytica complex of Pasteurella sensu stricto as new genus Mannheimia: changes in taxonomy

The benefit and usefulness of the new results described by Angen et al. (1999) rest upon the necessity for revising the previous taxonomy of Pasteurellaceae by grouping microorganisms based on their phylogenetic relationships combined with phenotypic characters and the usefulness of molecular biological methods in determining the taxonomic structure based on genomic evidence with supplementary phenotypic data. According to authors cited the phenotypic character of each serotype, species and even genus was well defined by their morphology, growth requirements and metabolic activities. The genomic relationships of serotypes, species and genera were detected by different molecular biological techniques.

Hematogenous pasteurella haemolytica brain abscess

ABSTRACT Pasteurella infections are common in domestic animals and very rare in human. We report a hematogenously acquired Pasteurella haemolytica brain abscess, mimicking brain tumor on magnetic resonance imaging, in an 18-year-old female patient known with cardiac interventricular communication, without recent history of animal contact. The outcome was good after abscess complete removal and antimicrobials therapy for 6 weeks. To the best of our knowledge, this is the first reported case of P. haemolytica brain abscess.