Microbiological and cytological characterization of coelomic fluid from three captive endangered amphibian Gastrotheca species with edema syndrome: preliminary analysis

Abstract Objective Edema syndrome is highly prevalent but under researched in captive frogs around the world. The objective of the present study was to characterize at a basic microbiological and cytological level of the bacteria of the edema fluid of 20 individuals of the genus Gastrotheca to determine the presence of possible anaerobic and aerobic bacteria. Results Fourteen types of bacteria were identified in the edema fluid, 12 of them at the species level (Pasteurella haemolytica, Hafnia alvei, Enterobacter agglomerans, Aeromonas hydrophila, Pseudomonas fluorescens, Burkholderia pseudomallei, Salmonella arizonae, Enterobacter gergoviae, Enterobacter sakazakii, Yersinia enterocolitica, Klebsiella oxytoca, and Klebsiella ozaenae) and two at the genus level (Enterococcus spp. and Streptococcus spp.). The most frequently identified cells were lymphocytes (37.7% in females and 46.4% in males), erythrocytes (23.5% in females and 17.5% in males) and neutrophils (4.2% in females and 2.8% in males). Finally, no relationship was found between the data obtained and the sex of the individuals studied.

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Microbiological and cytological characterization of coelomic fluid from three captive endangered amphibian Gastrotheca species with edema syndrome: Preliminary analysis.

10.21203/rs.2.14842/v2 ◽

2019 ◽

Author(s):

Anahí Oleas-Paz ◽

Ana Cecilia Santamaría-Naranjo ◽

Maira Rojas-Carrillo ◽

Andrés Merino-Viteri ◽

Alexander Genoy-Puerto

Keyword(s):

Burkholderia Pseudomallei ◽

Klebsiella Oxytoca ◽

Pasteurella Haemolytica ◽

Aerobic Bacteria ◽

Coelomic Fluid ◽

Enterobacter Sakazakii ◽

Enterobacter Agglomerans ◽

Edema Fluid ◽

Enterococcus Spp

Abstract Objective: Edema syndrome is highly prevalent but under researched in captive frogs around the world. The objective of the present study was to characterize at a basic microbiological and cytological level the edema fluid of 20 individuals of the genus Gastrotheca to determine the presence of possible anaerobic and aerobic bacteria. Results: Fourteen types of bacteria were identified in the edema fluid, twelve of them at the species level ( Pasteurella haemolytica , Hafnia alvei , Enterobacter agglomerans , Aeromonas hydrophila , Pseudomonas fluorescens , Burkholderia pseudomallei , Salmonella arizonae , Enterobacter gergoviae , Enterobacter sakazakii , Yersinia enterocolitica , Klebsiella oxytoca, and Klebsiella ozaenae ) and two at the genus level ( Enterococcus spp. and Streptococcus spp.). The most frequently identified cells were lymphocytes (37.7% in females and 46.4% in males), erythrocytes (23.5% in females and 17.5% in males) and neutrophils (4.2% in females and 2.8% in males). Finally, no relationship was found between the data obtained and the sex of the individuals studied.

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Microbiological and cytological characterization of coelomic fluid from three captive endangered amphibian Gastrotheca species with edema syndrome: Preliminary analysis.

10.21203/rs.2.14842/v1 ◽

2019 ◽

Author(s):

Anahí Oleas-Paz ◽

Ana Cecilia Santamaría-Naranjo ◽

Maira Rojas-Carrillo ◽

Andrés Merino-Viteri ◽

Alexander Genoy-Puerto

Keyword(s):

Burkholderia Pseudomallei ◽

Klebsiella Oxytoca ◽

Pasteurella Haemolytica ◽

Aerobic Bacteria ◽

Coelomic Fluid ◽

Enterobacter Sakazakii ◽

Enterobacter Agglomerans ◽

Edema Fluid ◽

Enterococcus Spp

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TOTAL AND FECAL COLIFORMS CONTAMINATION IN FAUCETS AND FLUSH BOTTOMS IN PUBLIC WASHROOMS SITED IN SHOPPING MALLS OF CURITIBA, STATE OF PARANA, BRAZIL

Estudos de Biologia ◽

10.7213/reb.v29i68/69.22784 ◽

2007 ◽

Vol 29 (68/69) ◽

Author(s):

Janaína Capelli Peixoto ◽

Sérgio Eduardo Fontoura-da-Silva

Keyword(s):

Klebsiella Oxytoca ◽

Fecal Contamination ◽

Fecal Coliforms ◽

Significant Other ◽

Enterobacter Sakazakii ◽

Enterobacter Agglomerans ◽

Shopping Malls ◽

Providencia Rettgeri ◽

Gram Negative ◽

Serratia Liquefaciens

A study was performed regarding the presence of fecal coliforms in faucets and flush bottoms in public restrooms in 10 shopping malls of Curitiba, Paraná, Brazil. The presence of these bacteria shows fecal contamination. Escherichia coli is the prototype of fecal coliforms that was found in 13,1% of the samples, being more common on faucets (21.9%) than on flush bottoms (4.6%), this difference was significant. Other enterobacteria have been found: Enterobacter agglomerans, Enterobacter sakazakii, Klebsiella oxytoca, Providencia alcafaciens, Providencia rettgeri and Serratia liquefaciens. These bacteria do not necessarily indicate fecal contamination. Nonfermentative gram negative bacilli (NFGNB) were found in 25 samples (43,9%), being more common over the flush bottoms (39,5%). The presence of fecal coliforms may indicate imperfection in the hygienic cleaning in these places, compromising the health of its users.

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Microbiological Examination of Vegetable Seed Sprouts in Korea

Journal of Food Protection ◽

10.4315/0362-028x-72.4.856 ◽

2009 ◽

Vol 72 (4) ◽

pp. 856-859 ◽

Cited By ~ 19

Author(s):

HOIKYUNG KIM ◽

YOUNGJUN LEE ◽

LARRY R. BEUCHAT ◽

BONG-JUNE YOON ◽

JEE-HOON RYU

Keyword(s):

Microbiological Quality ◽

Aerobic Bacteria ◽

Escherichia Coli O157 ◽

Enterobacter Sakazakii ◽

Department Stores ◽

E Coli ◽

Traditional Markets ◽

Radish Sprouts ◽

Online Stores

Sprouted vegetable seeds used as food have been implicated as sources of outbreaks of Salmonella and Escherichia coli O157:H7 infections. We profiled the microbiological quality of sprouts and seeds sold at retail shops in Seoul, Korea. Ninety samples of radish sprouts and mixed sprouts purchased at department stores, supermarkets, and traditional markets and 96 samples of radish, alfalfa, and turnip seeds purchased from online stores were analyzed to determine the number of total aerobic bacteria (TAB) and molds or yeasts (MY) and the incidence of Salmonella, E. coli O157:H7, and Enterobacter sakazakii. Significantly higher numbers of TAB (7.52 log CFU/g) and MY (7.36 log CFU/g) were present on mixed sprouts than on radish sprouts (6.97 and 6.50 CFU/g, respectively). Populations of TAB and MY on the sprouts were not significantly affected by location of purchase. Radish seeds contained TAB and MY populations of 4.08 and 2.42 log CFU/g, respectively, whereas populations of TAB were only 2.54 to 2.84 log CFU/g and populations of MY were 0.82 to 1.69 log CFU/g on alfalfa and turnip seeds, respectively. Salmonella and E. coli O157:H7 were not detected on any of the sprout and seed samples tested. E. sakazakii was not found on seeds, but 13.3% of the mixed sprout samples contained this potentially pathogenic bacterium.

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Microscopic Image Segmentation and Morphological Characterization of Novel Chitosan/Silica Nanoparticle/Nisin Films Using Antimicrobial Technique for Blueberry Preservation

Membranes ◽

10.3390/membranes11050303 ◽

2021 ◽

Vol 11 (5) ◽

pp. 303

Author(s):

Rokayya Sami ◽

Schahrazad Soltane ◽

Mahmoud Helal

Keyword(s):

Ph Value ◽

Silica Nanoparticle ◽

Morphological Characterization ◽

Aerobic Bacteria ◽

Nano Materials ◽

Elongation At Break ◽

Stable Suspension ◽

Average Size ◽

Material Ductility

In the current work, the characterization of novel chitosan/silica nanoparticle/nisin films with the addition of nisin as an antimicrobial technique for blueberry preservation during storage is investigated. Chitosan/Silica Nanoparticle/N (CH-SN-N) films presented a stable suspension as the surface loads (45.9 mV) and the distribution was considered broad (0.62). The result shows that the pH value was increased gradually with the addition of nisin to 4.12, while the turbidity was the highest at 0.39. The content of the insoluble matter and contact angle were the highest for the Chitosan/Silica Nanoparticle (CH-SN) film at 5.68%. The use of nano-materials in chitosan films decreased the material ductility, reduced the tensile strength and elongation-at-break of the membrane. The coated blueberries with Chitosan/Silica Nanoparticle/N films reported the lowest microbial contamination counts at 2.82 log CFU/g followed by Chitosan/Silica Nanoparticle at 3.73 and 3.58 log CFU/g for the aerobic bacteria, molds, and yeasts population, respectively. It was observed that (CH) film extracted 94 regions with an average size of 449.10, at the same time (CH-SN) film extracted 169 regions with an average size of 130.53. The (CH-SN-N) film presented the best result at 5.19%. It could be observed that the size of the total region of the fruit for the (CH) case was the smallest (1663 pixels), which implied that the fruit lost moisture content. As a conclusion, (CH-SN-N) film is recommended for blueberry preservation to prolong the shelf-life during storage.

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CSMD: a computational subtraction-based microbiome discovery pipeline for species-level characterization of clinical metagenomic samples

Bioinformatics ◽

10.1093/bioinformatics/btz790 ◽

2019 ◽

Author(s):

Yu Liu ◽

Paul W Bible ◽

Bin Zou ◽

Qiaoxing Liang ◽

Cong Dong ◽

...

Keyword(s):

Species Level ◽

Supplementary Information ◽

Clinical Samples ◽

Plasma Dna ◽

Bioinformatics Pipeline ◽

Microbial Identification ◽

Reference Databases ◽

Microbial Dna ◽

Higher Sensitivity

Abstract Motivation Microbiome analyses of clinical samples with low microbial biomass are challenging because of the very small quantities of microbial DNA relative to the human host, ubiquitous contaminating DNA in sequencing experiments and the large and rapidly growing microbial reference databases. Results We present computational subtraction-based microbiome discovery (CSMD), a bioinformatics pipeline specifically developed to generate accurate species-level microbiome profiles for clinical samples with low microbial loads. CSMD applies strategies for the maximal elimination of host sequences with minimal loss of microbial signal and effectively detects microorganisms present in the sample with minimal false positives using a stepwise convergent solution. CSMD was benchmarked in a comparative evaluation with other classic tools on previously published well-characterized datasets. It showed higher sensitivity and specificity in host sequence removal and higher specificity in microbial identification, which led to more accurate abundance estimation. All these features are integrated into a free and easy-to-use tool. Additionally, CSMD applied to cell-free plasma DNA showed that microbial diversity within these samples is substantially broader than previously believed. Availability and implementation CSMD is freely available at https://github.com/liuyu8721/csmd. Supplementary information Supplementary data are available at Bioinformatics online.

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Demonstration of the indolepyruvate decarboxylase gene homologue in different auxin-producing species of the Enterobacteriaceae

Canadian Journal of Microbiology ◽

10.1139/m94-170 ◽

1994 ◽

Vol 40 (12) ◽

pp. 1072-1076 ◽

Cited By ~ 17

Author(s):

Wolfgang Zimmer ◽

Barbara Hundeshagen ◽

Edith Niederau

Keyword(s):

Polymerase Chain Reaction ◽

Klebsiella Oxytoca ◽

Pyruvate Decarboxylase ◽

Enterobacter Agglomerans ◽

Chain Reaction ◽

Acetic Acid Production ◽

Conserved Regions ◽

Polymerase Chain ◽

Gene Homologue ◽

Indole 3 Acetic Acid

Different Enterobacteriaceae were assayed for their ability to produce the plant hormone indole-3-acetate with the aim to study the distribution of the indole-3-pyruvate pathway, which is known to be involved in the production of indole-3-acetate in a root-associated Enterobacter cloacae strain. Other E. cloacae strains, and also Enterobacter agglomerans strains, Pantoea agglomerans, Klebsiella aerogenes, and Klebsiella oxytoca were found to convert tryptophan into indole-3-acetate. As it was also intended to identify the conserved regions of the indole-3-pyruvate decarboxylase, which is involved in producing indole-3-acetate in the E. cloacae strain, oligonucleotide primers were synthesized for different regions of the corresponding gene. One pair of these primers allowed us to amplify a segment of the predicted size by the polymerase chain reaction with DNA of the seven different Enterobacteriaceae that produce indole-3-acetate. Segments of five strains were cloned and sequenced. All sequences showed significant homology to the indole-3-pyruvate decarboxylase gene. As in addition a positive DNA–DNA hybridization signal was detected in the seven strains using the E. cloacae or E. agglomerans segments as a probe, indole-3-acetate biosynthesis is suggested to be catalyzed via the indole-3-pyruvate pathway not only in E. cloacae but also in the other soil-living Enterobacteriaceae. Conserved regions were detected in the indole-3-decarboxylase by alignment of the now-available five different partial sequences. These regions should enable identification of the gene in other bacterial families or even in plants.Key words: indole-3-pyruvate decarboxylase, indole-3-acetic acid production, auxin, polymerase chain reaction, Enterobacteriaceae.

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Structural and Biophysical Characterization of the Burkholderia pseudomallei IspF Inhibitor L-Tryptophan Hydroxamate

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2021.128273 ◽

2021 ◽

pp. 128273

Author(s):

Joy M. Blain ◽

Dakota L. Grote ◽

Sydney M. Watkins ◽

Gashaw M. Goshu ◽

Chanté Muller ◽

...

Keyword(s):

Burkholderia Pseudomallei ◽

Biophysical Characterization

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Characterization of lesion formation in marmosets following inhalational challenge with different strains of Burkholderia pseudomallei

International Journal of Experimental Pathology ◽

10.1111/iep.12161 ◽

2015 ◽

Vol 96 (6) ◽

pp. 414-426 ◽

Cited By ~ 9

Author(s):

Michelle Nelson ◽

Alejandro Nunez ◽

Sarah A. Ngugi ◽

Adam Sinclair ◽

Timothy P. Atkins

Keyword(s):

Burkholderia Pseudomallei ◽

Lesion Formation ◽

Different Strains

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Molecular Identification and Morphological Characterization of Ficus sp. (Moraceae) in Bogor Botanic Gardens

Jurnal Biodjati ◽

10.15575/biodjati.v6i1.10852 ◽

2021 ◽

Vol 6 (1) ◽

pp. 36-44

Author(s):

Muhammad Rifqi Hariri ◽

Peniwidiyanti Peniwidiyanti ◽

Arifin Surya Dwipa Irsyam ◽

Rina Ratnasih Irwanto ◽

Irfan Martiansyah ◽

...

Keyword(s):

Molecular Identification ◽

Morphological Characterization ◽

Species Level ◽

Herbarium Specimen ◽

Morphological Observation ◽

Its Sequence ◽

Botanic Gardens ◽

Molecular Approaches ◽

Vegetative Characters

Ficus spp. belongs to the tribe Ficeae in the Moraceae family. Many members of this genus have been collected and grown in Bogor Botanic Gardens. There are 519 living collections of Ficus conserved since 1817, and 13 of them have not been identified until the species level. This research aimed to identify the Ficus sp. originated from Kaur Selatan (Bengkulu) using morphological and molecular approaches. Morphological characterization and herbarium specimen observation have been carried out to identify the Ficus sp. The molecular approach was conducted through DNA barcoding using ITS primer. The molecular identification using ITS sequence showed that Ficus sp. is Ficus crassiramea with 99.87% similarity to the sequence in NCBI. Morphological observation through herbarium specimen showed that there are 9 vegetative characters specific to Ficus crassiramea.

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