In vitro culture ofBellevalia romana (L.) Rchb. I. Plant regeneration through adventitious shoots and somatic embryos

The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.

Download Full-text

In Vitro Culture and Plant Regeneration in Gramineous Crops

Current Issues in Plant Molecular and Cellular Biology - Current Plant Science and Biotechnology in Agriculture ◽

10.1007/978-94-011-0307-7_6 ◽

1995 ◽

pp. 59-68 ◽

Cited By ~ 2

Author(s):

B. V. Conger ◽

A. I. Kuklin

Keyword(s):

Plant Regeneration ◽

In Vitro Culture

Download Full-text

In vitro culture of pointed gourd (Trichosanthes dioica Roxb.)

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v35i1.5874 ◽

1970 ◽

Vol 35 (1) ◽

pp. 135-142 ◽

Cited By ~ 2

Author(s):

MA Malek ◽

D Khanam ◽

M Khatun ◽

MH Molla ◽

MA Mannan

Keyword(s):

Plant Regeneration ◽

In Vitro Culture ◽

Culture Media ◽

Culture Conditions ◽

Root Induction ◽

Ms Medium ◽

Trichosanthes Dioica ◽

Pointed Gourd ◽

Regenerated Plantlets

An experiment was conducted to study the in vitro culture of pointed gourd. Cotyledon rescued from physiologically matured seeds (PMS) and immatured seeds (IMS) of pointed gourd were used as explants. Cotyledon excised from PMS responded very well in all culture conditions. Plant regenerated from cotyledon of PMS ranged from 38 to 96% in different hormonal formulations of culture media. Highest percentage of shoot regeneration was observed in MS + 1.0 mg/l BAP and lowest in MS + 2.5 mg/l BAP. No plant regeneration was observed in cotyledon from immatured seeds. The highest percentage of root induction (99%) was achieved in half MS medium supplemented with 0.5 mg/l NAA. The regenerated plantlets were successfully established in earthen pot. Keywords: Cotyledon; in vitro; pointed gourd. DOI: 10.3329/bjar.v35i1.5874Bangladesh J. Agril. Res. 35(1) : 135-142, March 2010

Download Full-text

In vitro Culture of White Clover: Callus, Suspension, Protoplast Culture, and Plant Regeneration

Botanical Gazette ◽

10.1086/337138 ◽

1980 ◽

Vol 141 (2) ◽

pp. 157-164 ◽

Cited By ~ 72

Author(s):

Peter M. Gresshoff

Keyword(s):

Plant Regeneration ◽

In Vitro Culture ◽

White Clover ◽

Protoplast Culture

Download Full-text

883 PB 279 REGENERATING ADVENTITIOUS PLANTS FROM IN VITRO CULTURE OF LIATRIS SPICATA (L.) WILLD. COTYLEDONS

HortScience ◽

10.21273/hortsci.29.5.560d ◽

1994 ◽

Vol 29 (5) ◽

pp. 560d-560

Author(s):

Dennis P. Stimart ◽

John C. Mather

Keyword(s):

In Vitro Culture ◽

Adventitious Shoots ◽

Adventitious Shoot ◽

Shoot Formation ◽

Ms Medium ◽

Ex Vitro ◽

Adventitious Shoot Formation ◽

Murashige Skoog ◽

Micropropagated Plants

Cotyledons from developing embryos 6 to 8 weeks old of Liatris spicata (blazing star) were cultured on Murashige-Skoog (MS) medium containing 0, 0.4, 4.4, and 44.4 μ M benzyladenine (BA) or 0, 0.2, 2.2, and 22.2 μ M thidiazuron (TDZ) to induce adventitious shoot formation. The highest percent of cotyledons forming shoots with highest shoot counts was on medium containing 2.2 μ M TDZ. Vitreous shoots formed on medium with 22.2 μ M TDZ. Callus derived from cotyledons and cultured on medium containing 4.44 μ M BA or 2.2 μ M TDZ formed adventitious shoots with highest shoot counts on 4.44 μ M BA. Adventitious shoots derived from cotyledons and callus were rooted on MS medium with 5.0 μ Mindole-3-butyric acid, acclimatized and grown ex vitro. All micropropagated plants appeared similar to each other.

Download Full-text

Regenerating Adventitious Shoots from in Vitro Culture of Liatris spicata (L.) Willd. Cotyledons

HortScience ◽

10.21273/hortsci.31.1.154 ◽

1996 ◽

Vol 31 (1) ◽

pp. 154-155

Author(s):

Dennis P. Stimart ◽

John C. Mather

Keyword(s):

In Vitro Culture ◽

Adventitious Shoots ◽

Adventitious Shoot ◽

Shoot Formation ◽

Ms Medium ◽

Adventitious Shoot Formation ◽

Regenerated Plants ◽

Murashige And Skoog Medium ◽

Homogeneous Chemical

Cotyledons from developing 6- to 8-week-old embryos of Liatris spicata (L.) Willd. (blazing star) were cultured on Murashige and Skoog medium containing 0, 0.4, 4.4, or 44.4 μm BA or 0, 0.2, 2.2, or 22.2 μm TDZ to induce adventitious shoot formation. The highest percentage of cotyledons forming the most shoots was on medium containing 2.2 μm TDZ. Cotyledon-derived callus cultured on medium containing 4.4 μm BA formed ≈16 times more adventitious shoots than on 2.2 μm TDZ. Adventitious shoots derived from cotyledons or callus produced roots when placed on MS medium containing 5.0 μm IBA. Regenerated plants that flowered in the field appeared homogeneous. Chemical names used: N6-benzyladenine (BA), thidiazuron (TDZ), indole-3-butyric acid (IBA).

Download Full-text

331 Effects of Explant Type, Sucrose Level and Callus Development Time on In Vitro Plant Regeneration of Sweetpotato

HortScience ◽

10.21273/hortsci.35.3.449a ◽

2000 ◽

Vol 35 (3) ◽

pp. 449A-449

Author(s):

L. Chen ◽

S.O. Park ◽

S. Dhir ◽

A.S. Bhagsari

Keyword(s):

Plant Regeneration ◽

Plant Growth ◽

Growth Regulators ◽

Somatic Embryos ◽

Development Time ◽

Shoot Tip ◽

Explant Type ◽

Callus Initiation ◽

Sucrose Level

Several experiments were conducted to evaluate the influence of explant type, sucrose level, and callus development time on sweetpotato [Ipomoea batatas (L.) Lam] in vitro culture. Shoot tip, petiole, and leaf of Selection 75-96-1 was used as explants in Murashige and Skoog (MS) media with different plant growth regulators. Calli derived from shoot tip and petiole produced 42.1% and 10.3% somatic embryos, respectively, but the leaf failed to produce somatic embryos. The effect of sucrose level was determined using shoot tip as explants. Compared with 3% sucrose in the same plant growth regulators level medium during callus initiation and callus proliferation periods, 5% sucrose level suppressed root growth and improved shoot regeneration. The callus development time was measured by using shoot tips on callus initiation medium containing 1.5 mg/L alpha-Naphthaleneacetic acid (NAA) and 0.25 mg/L Kinetin (KIN) plus 5% sucrose. When explants were cultured for less than 6 weeks during callus initiation, then transferred onto plant regeneration medium, plant regeneration via organogenesis occurred; whereas, maintaining cultures for more than 12 weeks on the same callus initiation/proliferation medium, plant regeneration was favored via embryogenesis. Explant type and other factors affecting plant regeneration noted here could be applied to protoplast culture, somatic hybridization, and transformation in sweetpotato.

Download Full-text