Mechanism of transport of vegetative storage proteins to the vacuole of the paraveinal mesophyll of soybean leaf

PROTOPLASMA ◽  
1997 ◽  
Vol 200 (3-4) ◽  
pp. 174-185 ◽  
Author(s):  
Stephen F. Klauer ◽  
Vincent R. Franceschi
Keyword(s):  
Storage Proteins ◽  
Vegetative Storage Proteins ◽  
Paraveinal Mesophyll ◽  
Soybean Leaf

The functional status of paraveinal mesophyll vacuoles changes in response to altered metabolic conditions in soybean leaves

2005 ◽  
Vol 32 (4) ◽  
pp. 335 ◽  
Author(s):  
Kimberly A. Murphy ◽  
Rachel A. Kuhle ◽  
Andreas M. Fischer ◽  
Aldwin M. Anterola ◽  
Howard D. Grimes
Keyword(s):  
Functional Status ◽  
Storage Proteins ◽  
Vegetative Storage Proteins ◽  
Paraveinal Mesophyll ◽  
Lytic Function ◽  
Metabolic Conditions ◽  
Glycine Max L ◽  
Soybean Leaves ◽  
And Storage ◽  
Lytic Compartment

Antibodies raised against tonoplast intrinsic proteins (TIPs) were used to probe the functional status of the soybean [Glycine max (L.) Merr.] paraveinal mesophyll (PVM) vacuole during changes in nitrogen metabolism within the leaf. Young plants grown under standard conditions had PVM vacuoles characterised by the presence of γ-TIP, which is indicative of a lytic function. When plants were then subjected to shoot tip removal for a period of 15 d, forcing a sink-limited physiological condition, the γ-TIP marker diminished while the δ-TIP marker became present in the PVM vacuole, indicating the conversion of the PVM vacuole to a storage function. When the shoot tips were allowed to regrow, the γ-TIP marker again became dominant demonstrating the reversion of these PVM vacuoles back to a lytic compartment. The changes in TIP markers correlated with the accumulation of vegetative storage proteins and vegetative lipoxygenases, proteins implicated in nitrogen storage and assimilate partitioning. This research suggests that the PVM vacuole is able to undergo dynamic conversion between lytic and storage functions and further implicates this cell layer in assimilate storage and mobilisation in soybeans.

Soybean vegetative lipoxygenases are not vacuolar storage proteins

2011 ◽  
Vol 38 (10) ◽  
pp. 778 ◽  
Author(s):  
Glenn W. Turner ◽  
Howard D. Grimes ◽  
B. Markus Lange
Keyword(s):  
Storage Proteins ◽  
Tissue Level ◽  
Protein Bodies ◽  
Specific Antibodies ◽  
Vegetative Storage Proteins ◽  
Paraveinal Mesophyll ◽  
Cast Doubt ◽  
Sink Limitation ◽  
Immuno Cytochemistry

The paraveinal mesophyll (PVM) of soybean is a distinctive uniseriate layer of branched cells situated between the spongy and palisade chlorenchyma of leaves that contains an abundance of putative vegetative storage proteins, Vspα and Vspβ, in its vacuoles. Soybean vegetative lipoxygenases (five isozymes designated as Vlx(A–E)) have been reported to co-localise with Vsp in PVM vacuoles; however, conflicting results regarding the tissue-level and subcellular localisations of specific Vlx isozymes have been reported. We employed immuno-cytochemistry with affinity-purified, isozyme-specific antibodies to reinvestigate the subcellular locations of soybean Vlx isozymes during a sink limitation experiment. VlxB and VlxC were localised to the cytoplasm and nucleoplasm of PVM cells, whereas VlxD was present in the cytoplasm and nucleoplasm of mesophyll chlorenchyma (MC) cells. Label was not associated with storage vacuoles or any evident protein bodies, so our results cast doubt on the hypothesis that Vlx isozymes function as vegetative storage proteins.

Expression of Soybean Vegetative Storage Proteins (VSPα) in Tobacco Leaves

1999 ◽  
pp. 293-296
Author(s):  
D. Guenoune ◽  
R. Amir ◽  
S. Wolf ◽  
S. Wininger ◽  
H. Badani ◽  
...  
Keyword(s):  
Storage Proteins ◽  
Tobacco Leaves ◽  
Vegetative Storage Proteins

Effect of drought, elevated CO2 and temperature on accumulation of N and vegetative storage proteins (VSP) in taproot of nodulated alfalfa before and after cutting

Plant Science ◽  
2007 ◽  
Vol 172 (5) ◽  
pp. 903-912 ◽  
Author(s):  
Gorka Erice ◽  
Juan José Irigoyen ◽  
Manuel Sánchez-Díaz ◽  
Jean-Christophe Avice ◽  
Alain Ourry
Keyword(s):  
Elevated Co2 ◽  
Storage Proteins ◽  
Vegetative Storage Proteins ◽  
Before And After ◽  
Elevated Co2 And Temperature

DISTRIBUTION OF VEGETATIVE STORAGE PROTEINS IN ROSACEAE

IAWA Journal ◽  
2003 ◽  
Vol 24 (4) ◽  
pp. 421-428
Author(s):  
Wei-Min Tian ◽  
Zheng-Hai Hu
Keyword(s):  
Seasonal Changes ◽  
Storage Proteins ◽  
Protein Body ◽  
Diagnostic Feature ◽  
Secondary Phloem ◽  
Apple Trees ◽  
Phloem Parenchyma ◽  
Vegetative Storage Proteins ◽  
Parenchyma Cells ◽  
First Time

The distribution pattern of vegetative storage proteins is reported for the first time for 18 species and 2 varieties of twelve genera of Rosaceae. Vegetative storage proteins were present in all the species studied of Prunoideae and absent in Maloideae. Their occurrence in a genus seemed to be either universal or entirely absent. Rosaceae trees were poor in vegetative storage proteins and the form of vegetative storage proteins was not protein body-like. Granular and floccular forms of vegetative storage proteins could be distinguished exclusively in the secondary phloem parenchyma cells and their distribution was cell-specific. Our results suggest that the distribution of vegetative storage proteins in Rosaceae can be considered as a taxonomically diagnostic feature. The nature of the bark proteins with seasonal changes in apple trees is discussed.

Root storage proteins, with particular reference to taproots

2002 ◽  
Vol 80 (4) ◽  
pp. 321-329 ◽  
Author(s):  
J Derek Bewley
Keyword(s):  
Total Nitrogen ◽  
Storage Proteins ◽  
Minor Component ◽  
Early Summer ◽  
Crop Species ◽  
Nitrogen Pool ◽  
Vegetative Storage Proteins ◽  
Perennial Weeds ◽  
Nitrogen Pools ◽  
Root Proteins

The presence of storage proteins has been reported in roots of several perennial and biennial weed and crop species, and particularly in members of the Compositae, Euphorbiaceae, and Leguminosae. In some species the amount of these root proteins fluctuates seasonally, increasing in the fall and winter months and declining in the spring and early summer. Also, the root proteins may decline during regrowth of decapitated plants. The evidence that these proteins play a role as storage proteins is frequently only circumstantial; moreover, they are usually only a relatively minor component of the total nitrogen pool within the root. Only one root protein, that from the dandelion taproot, has been extensively characterized, and it has no properties in common with known vegetative storage proteins. The literature on root proteins is reviewed, with particular emphasis on those present in taproots. The paucity of definitive data allows few conclusions to be reached, and more research is required to establish the role, nature, and importance of root proteins.Key words: taproots, perennial weeds, root proteins, nitrogen pools, storage proteins.

Biochemical and Immunological Investigations on Vegetative Storage Proteins of Taxodiaceae Species

1994 ◽  
Vol 143 (6) ◽  
pp. 601-608 ◽  
Author(s):  
Ute Harms ◽  
Jörg J. Sauter
Keyword(s):  
Storage Proteins ◽  
Vegetative Storage Proteins
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