Comparison of human and porcine group C rotaviruses by Northern blot hybridization analysis

1991 ◽  
Vol 118 (3-4) ◽  
pp. 269-277 ◽  
Author(s):  
Y. Qian ◽  
L. J. Saif ◽  
A. Z. Kapikian ◽  
S. Y. Kang ◽  
B. Jiang ◽  
...  
Keyword(s):  
Northern Blot ◽  
Blot Hybridization ◽  
Hybridization Analysis ◽  
Northern Blot Hybridization

Analysis of RNA by Northern-Blot Hybridization

2003 ◽  
pp. 205-213 ◽  
Author(s):  
Patricia E. Gallagher ◽  
Debra I. Diz
Keyword(s):  
Northern Blot ◽  
Blot Hybridization ◽  
Northern Blot Hybridization

scholarly journals Induction of hepatic metallothioneins determined at isoprotein and messenger RNA levels in glucocorticoid-treated rats

1988 ◽  
Vol 249 (2) ◽  
pp. 429-433 ◽  
Author(s):  
L D Lehman-McKeeman ◽  
G K Andrews ◽  
C D Klaassen
Keyword(s):  
Detection Limit ◽  
Northern Blot ◽  
Blot Analysis ◽  
Messenger Rna ◽  
Time Course ◽  
Northern Blot Analysis ◽  
Blot Hybridization ◽  
Northern Blot Hybridization ◽  
Single Dosage ◽  
Rna Levels

Induction of metallothionein-I (MT-I) and metallothionein-II (MT-II) by glucocorticoids was determined by h.p.l.c. analysis of proteins and Northern-blot analysis of MT mRNAs. Rats were injected with dexamethasone (0.03-10 mumol/kg) and hepatic concentrations of MTs were determined 24 h later. In control rats, only MT-II was detected (9.4 +/- 2.5 micrograms/g of liver), whereas the hepatic concentration of MT-I was below the detection limit (5 micrograms of MT/g). Dexamethasone did not increase MT-I above the detection limit at any dosage tested, but MT-II increased to 2.5 times control values at dosages of 0.30 mumol/kg and higher. Time-course experiments indicated that MT-II reached a maximum at 24 h after a single dosage of dexamethasone and returned to control values by 48 h. To determine whether dexamethasone increased MT-I in liver, samples were saturated with 109Cd, after which the amount of 109Cd in MT-I and MT-II was determined. Results indicated that, by this approach, MT-I and MT-II could be detected in control rats, and there was approx. 1.8 times more 109Cd in MT-II than in MT-I. At 24 h after administration of dexamethasone (1 mumol/kg), there was a small increase in the amount of 109Cd bound to MT-I, whereas the amount of 109Cd bound to MT-II increased to more than 2 times control values. Northern-blot hybridization with mouse cRNA probes indicated that MT-I and MT-II mRNAs increased co-ordinately after administration of dexamethasone. Thus, although glucocorticoids increase both MT-I and MT-II mRNAs, MT-II preferentially accumulates after administration of dexamethasone.

Fgf-4 expression during gastrulation, myogenesis, limb and tooth development in the mouse

Development ◽  
1992 ◽  
Vol 114 (3) ◽  
pp. 755-768 ◽  
Author(s):  
L. Niswander ◽  
G.R. Martin
Keyword(s):  
Tooth Development ◽  
Inner Cell Mass ◽  
Expression Patterns ◽  
Blot Hybridization ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Hybridization Analysis ◽  
Limb Bud ◽  
Northern Blot Hybridization ◽  
Tail Bud

Fgf-4, initially isolated as a transforming gene from human tumors, is a member of the Fibroblast Growth Factor (FGF) family. It has previously been shown by northern blot hybridization analysis to be expressed in teratocarcinoma and embryonic stem cells, suggesting that it plays a role in embryonic development. We have carried out an RNA in situ hybridization analysis of Fgf-4 expression in the developing mouse embryo, from fertilization through the 14th day of gestation (E14.5). Our results show that Fgf-4 RNA is first detected at the late blastocyst stage in cells that give rise to all of the embryonic lineages (inner cell mass cells). During the early stages of gastrulation, expression becomes restricted to the primitive streak where mesoderm and definitive endoderm are formed. Expression continues in the distal (rostral) two-thirds of the streak through approx. E10, and then is detected in the tail bud, which replaces the streak as the primary source of mesoderm. Additional sites of expression are found after the three primary germ layers are established and organogenesis begins. Fgf-4 RNA is detected transiently in the branchial arch units, the somitic myotome, the apical ectodermal ridge of the developing limb bud and the tooth bud, suggesting that the gene has multiple roles during embryogenesis. These results are compared with the expression patterns of other FGF genes. Taken together, the data suggest that individual members of the gene family are expressed sequentially in developmental pathways such as mesoderm formation and myogenesis, and play a role in specific epithelial-mesenchymal interactions.

Modulable Southern and Northern blot hybridization apparatus for routine screening of gene amplification and expression

1990 ◽  
Vol 20 (4) ◽  
pp. 293-302
Author(s):  
Christophe Bignon ◽  
Magali Roux-Dosseto ◽  
Jean-Claude Lissitzky ◽  
Pierre-Marie Martin
Keyword(s):  
Gene Amplification ◽  
Northern Blot ◽  
Blot Hybridization ◽  
Routine Screening ◽  
Northern Blot Hybridization
Sign in / Sign up

Export Citation Format

Share Document