Population genetics and systematics of theLeptasterias hexactis (Echinodermata: Asteroidea) species complex

1990 ◽  
Vol 105 (3) ◽  
pp. 477-489 ◽  
Author(s):  
K. E. Kwast ◽  
D. W. Foltz ◽  
W. B. Stickle

10.5772/35584 ◽  
2012 ◽  
Author(s):  
M Carmen ◽  
Maribel Farfn ◽  
David Miana-Galbis ◽  
Vicenta Albarral ◽  
Ariadna Sanglas ◽  
...  


2016 ◽  
Vol 18 (3) ◽  
pp. 553-572 ◽  
Author(s):  
Michelle A. Duennes ◽  
Chris Petranek ◽  
Esteban Pineda Diez de Bonilla ◽  
Jorge Mérida-Rivas ◽  
Oscar Martinez-López ◽  
...  


2013 ◽  
Vol 14 (5) ◽  
pp. 1019-1028 ◽  
Author(s):  
Jan Christian Habel ◽  
Siobhan Cox ◽  
Franz Gassert ◽  
Ronald K. Mulwa ◽  
Jobst Meyer ◽  
...  


2016 ◽  
Vol 83 (5) ◽  
Author(s):  
Jérémy Guinard ◽  
Anne Latreille ◽  
Fabien Guérin ◽  
Stéphane Poussier ◽  
Emmanuel Wicker

ABSTRACT Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) is considered one of the most harmful plant diseases in the world. Special attention should be paid to R. pseudosolanacearum phylotype I due to its large host range, its worldwide distribution, and its high evolutionary potential. So far, the molecular epidemiology and population genetics of this bacterium are poorly understood. Until now, the genetic structure of the RSSC has been analyzed on the worldwide and regional scales. Emerging questions regarding evolutionary forces in RSSC adaptation to hosts now require genetic markers that are able to monitor RSSC field populations. In this study, we aimed to evaluate the multilocus variable-number tandem-repeat analysis (MLVA) approach for its ability to discriminate genetically close phylotype I strains and for population genetics studies. We developed a new MLVA scheme (MLVA-7) allowing us to genotype 580 R. pseudosolanacearum phylotype I strains extracted from susceptible and resistant hosts and from different habitats (stem, soil, and rhizosphere). Based on specificity, polymorphism, and the amplification success rate, we selected seven fast-evolving variable-number tandem-repeat (VNTR) markers. The newly developed MLVA-7 scheme showed higher discriminatory power than the previously published MLVA-13 scheme when applied to collections sampled from the same location on different dates and to collections from different locations on very small scales. Our study provides a valuable tool for fine-scale monitoring and microevolution-related study of R. pseudosolanacearum phylotype I populations. IMPORTANCE Understanding the evolutionary dynamics of adaptation of plant pathogens to new hosts or ecological niches has become a key point for the development of innovative disease management strategies, including durable resistance. Whereas the molecular mechanisms underlying virulence or pathogenicity changes have been studied thoroughly, the population genetics of plant pathogen adaptation remains an open, unexplored field, especially for plant-pathogenic bacteria. MLVA has become increasingly popular for epidemiosurveillance and molecular epidemiology studies of plant pathogens. However, this method has been used mostly for genotyping and identification on a regional or global scale. In this study, we developed a new MLVA scheme, targeting phylotype I of the soilborne Ralstonia solanacearum species complex (RSSC), specifically to address the bacterial population genetics on the field scale. Such a MLVA scheme, based on fast-evolving loci, may be a tool of choice for field experimental evolution and spatial genetics studies.



Author(s):  
Norio Kobayashi ◽  
Momoko Kumagai ◽  
Daisuke Minegishi ◽  
Koichiro Tamura ◽  
Tadashi Aotsuka ◽  
...  


2014 ◽  
Vol 25 (1-2) ◽  
pp. 61-68 ◽  
Author(s):  
V. I. Monchenko ◽  
L. P. Gaponova ◽  
V. R. Alekseev

Crossbreeding experiments were used to estimate cryptic species in water bodies of Ukraine and Russia because the most useful criterion in species independence is reproductive isolation. The problem of cryptic species in the genus Eucyclops was examined using interpopulation crosses of populations collected from Baltic Sea basin (pond of Strelka river basin) and Black Sea basin (water-reservoires of Dnieper, Dniester and Danube rivers basins). The results of reciprocal crosses in Eucyclops serrulatus-group are shown that E. serrulatus from different populations but from water bodies belonging to the same river basin crossed each others successfully. The interpopulation crosses of E. serrulatus populations collected from different river basins (Dnipro, Danube and Dniester river basins) were sterile. In this group of experiments we assigned evidence of sterility to four categories: 1) incomplete copulation or absence of copulation; 2) nonviable eggs; 3) absence of egg membranes or egg sacs 4) empty egg membranes. These crossbreeding studies suggest the presence of cryptic species in the E. serrulatus inhabiting ecologically different populations in many parts of its range. The same crossbreeding experiments were carries out between Eucyclops serrulatus and morphological similar species – Eucyclops macruroides from Baltic and Black Sea basins. The reciprocal crossings between these two species were sterile. Thus taxonomic heterogeneity among species of genus Eucyclops lower in E. macruroides than in E. serrulatus. The interpopulation crosses of E. macruroides populations collected from distant part of range were fertile. These crossbreeding studies suggest that E. macruroides species complex was evaluated as more stable than E. serrulatus species complex.



Sign in / Sign up

Export Citation Format

Share Document