scholarly journals Population Genetics of the “Aeromonas hydrophila Species Complex”

10.5772/35584 ◽  
2012 ◽  
Author(s):  
M Carmen ◽  
Maribel Farfn ◽  
David Miana-Galbis ◽  
Vicenta Albarral ◽  
Ariadna Sanglas ◽  
...  

2016 ◽  
Vol 18 (3) ◽  
pp. 553-572 ◽  
Author(s):  
Michelle A. Duennes ◽  
Chris Petranek ◽  
Esteban Pineda Diez de Bonilla ◽  
Jorge Mérida-Rivas ◽  
Oscar Martinez-López ◽  
...  


2013 ◽  
Vol 14 (5) ◽  
pp. 1019-1028 ◽  
Author(s):  
Jan Christian Habel ◽  
Siobhan Cox ◽  
Franz Gassert ◽  
Ronald K. Mulwa ◽  
Jobst Meyer ◽  
...  


1990 ◽  
Vol 105 (3) ◽  
pp. 477-489 ◽  
Author(s):  
K. E. Kwast ◽  
D. W. Foltz ◽  
W. B. Stickle


2008 ◽  
pp. 7-11
Author(s):  
László Ardó ◽  
János Bakos ◽  
Zoltán Tamás Nagy ◽  
András Rónyai ◽  
Dénes Gál ◽  
...  

Septicemia disease caused by the bacterium Aeromonas hydrophila is a major problem for fish farmers around the world. In our experiment resistance to the disease was investigated on carp variants with diferent genetical backgrounds. 96 different genetic variants(„families”) were created by crossing various individuals of 4 carp strains (2 wild and 2 cultured ones). Families were reared until reaching the size of 10 gramms and 100 fish of each families were marked with an individual electronic identifier. Marked fish were divided into 5 groups. Fish from one group were infected with A. hydrophila and mortality were registered during 2 weeks. Based on mortality data, the most resistant and most susceptible families will be selected using population genetics methods.



2016 ◽  
Vol 83 (5) ◽  
Author(s):  
Jérémy Guinard ◽  
Anne Latreille ◽  
Fabien Guérin ◽  
Stéphane Poussier ◽  
Emmanuel Wicker

ABSTRACT Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) is considered one of the most harmful plant diseases in the world. Special attention should be paid to R. pseudosolanacearum phylotype I due to its large host range, its worldwide distribution, and its high evolutionary potential. So far, the molecular epidemiology and population genetics of this bacterium are poorly understood. Until now, the genetic structure of the RSSC has been analyzed on the worldwide and regional scales. Emerging questions regarding evolutionary forces in RSSC adaptation to hosts now require genetic markers that are able to monitor RSSC field populations. In this study, we aimed to evaluate the multilocus variable-number tandem-repeat analysis (MLVA) approach for its ability to discriminate genetically close phylotype I strains and for population genetics studies. We developed a new MLVA scheme (MLVA-7) allowing us to genotype 580 R. pseudosolanacearum phylotype I strains extracted from susceptible and resistant hosts and from different habitats (stem, soil, and rhizosphere). Based on specificity, polymorphism, and the amplification success rate, we selected seven fast-evolving variable-number tandem-repeat (VNTR) markers. The newly developed MLVA-7 scheme showed higher discriminatory power than the previously published MLVA-13 scheme when applied to collections sampled from the same location on different dates and to collections from different locations on very small scales. Our study provides a valuable tool for fine-scale monitoring and microevolution-related study of R. pseudosolanacearum phylotype I populations. IMPORTANCE Understanding the evolutionary dynamics of adaptation of plant pathogens to new hosts or ecological niches has become a key point for the development of innovative disease management strategies, including durable resistance. Whereas the molecular mechanisms underlying virulence or pathogenicity changes have been studied thoroughly, the population genetics of plant pathogen adaptation remains an open, unexplored field, especially for plant-pathogenic bacteria. MLVA has become increasingly popular for epidemiosurveillance and molecular epidemiology studies of plant pathogens. However, this method has been used mostly for genotyping and identification on a regional or global scale. In this study, we developed a new MLVA scheme, targeting phylotype I of the soilborne Ralstonia solanacearum species complex (RSSC), specifically to address the bacterial population genetics on the field scale. Such a MLVA scheme, based on fast-evolving loci, may be a tool of choice for field experimental evolution and spatial genetics studies.



Author(s):  
Norio Kobayashi ◽  
Momoko Kumagai ◽  
Daisuke Minegishi ◽  
Koichiro Tamura ◽  
Tadashi Aotsuka ◽  
...  


1981 ◽  
Vol 117 (4) ◽  
pp. 244-244 ◽  
Author(s):  
D. F. Young
Keyword(s):  




Sign in / Sign up

Export Citation Format

Share Document