Microwave irradiation improvements in the silver staining of the nucleolar organizer (Ag-NOR) technique

1995 ◽  
Vol 103 (6) ◽  
pp. 403-413 ◽  
Author(s):  
Francisco-Javier Medina ◽  
Antonio Cerdido ◽  
Roberto Marco
Keyword(s):  
Microwave Irradiation ◽  
Silver Staining ◽  
Nucleolar Organizer

Sequential silver staining and in situ hybridization reveal a direct association between rDNA levels and the expression of homologous nucleolar organizing regions: a hypothesis for NOR structure and function

1998 ◽  
Vol 111 (10) ◽  
pp. 1433-1439
Author(s):  
F. Zurita ◽  
R. Jimenez ◽  
M. Burgos ◽  
R.D. de la Guardia
Keyword(s):  
Transcription Factors ◽  
In Situ Hybridization ◽  
Silver Staining ◽  
Organizer Region ◽  
Nucleolar Organizer ◽  
Interindividual Variation ◽  
Nucleolar Organizer Regions ◽  
Single Pair ◽  
Ribosomal Cistrons

We have developed a procedure for sequential silver staining and in situ hybridization to analyze the relationship between the amount of rDNA present in nucleolar organizer regions, as estimated by in situ hybridization, and their level of expression, as estimated by the silver signal. For simplicity we used cells from the insectivorous mole Talpa occidentalis, which have a single pair of nucleolar organizer regions in chromosome pair 3. The relative content of ribosomal cistrons was also related to the hierarchy of activation of the nucleolar organizer regions present in this chromosomal pair. Statistical analyses demonstrated that both the relative level of expression and the activation hierarchy depended mainly on the number of ribosomal cistrons in nucleolar organizer regions. We propose a functional two-step hypothesis, which is consistent with most known data concerning interchromosomal, intercellular and interindividual variation in a number of plant and animal species, including Talpa occidentalis. In step one, the first available transcription factors bind randomly to the ribosomal promoters, such that larger nucleolar organizer regions are more likely to recruit them. In the second step the remaining transcription factors are recruited in a cooperative way, thus completing activation of one nucleolar organizer region, before the next one becomes active.

A rapid immunogold-silver staining for detection of bromodeoxyuridine in large numbers of plastic sections, using microwave irradiation

1990 ◽  
Vol 22 (6-7) ◽  
pp. 321-326 ◽  
Author(s):  
H. J. G. Van De Kant ◽  
A. M. M. Van Pelt ◽  
R. P. F. A. Vergouwen ◽  
D. G. De Rooij
Keyword(s):  
Microwave Irradiation ◽  
Silver Staining ◽  
Large Numbers

Immunogold – Silver Staining Using Microwave Irradiation

1991 ◽  
pp. 347-367
Author(s):  
MATHILDE E. BOON ◽  
H.J.G. VAN DE KANT ◽  
L.P. KOK
Keyword(s):  
Microwave Irradiation ◽  
Silver Staining

Nucleolar organizer activity in wheat, rye and derivatives analyzed by a silver-staining procedure

Chromosoma ◽  
1984 ◽  
Vol 89 (5) ◽  
pp. 370-376 ◽  
Author(s):  
M. C. Cerme�o ◽  
J. Orellana ◽  
J. L. Santos ◽  
J. R. Lacadena
Keyword(s):  
Silver Staining ◽  
Nucleolar Organizer ◽  
Staining Procedure ◽  
Nucleolar Organizer Activity ◽  
Organizer Activity

NUCLEOLAR ORGANIZER REGIONS IN THE RABBIT (ORYCTOLAGUS CUNICULUS) AS SHOWN BY SILVER STAINING

1978 ◽  
Vol 20 (3) ◽  
pp. 377-382 ◽  
Author(s):  
Patricia A. Martin-Deleon ◽  
Dorene L. Petrosky ◽  
M. Eileen Fleming
Keyword(s):  
New Zealand ◽  
Silver Staining ◽  
Oryctolagus Cuniculus ◽  
Nucleolar Organizer ◽  
Nucleolar Organizer Regions ◽  
Telomeric Region ◽  
Metaphase Chromosomes ◽  
Domestic Rabbit ◽  
Silver Precipitation ◽  
Secondary Constrictions

Nucleolar organizer regions (NOR's) were demonstrated in metaphase chromosomes of the domestic rabbit, Oryctolagus cuniculus (L.) (New Zealand white strain) using silver staining. Sequential quinacrine banding and a modification of the Ag-AS silver precipitation technique with duplicate photography allowed identification of silver staining NOR's on the short arms of chromosomes 13, 16, and 20, as well as the telomeric region of the long arms of number 21 in some cells. Chromosomes 13, 16 and 20 all have subterminal to terminal centromeres, often showed satellites and secondary constrictions, and were sometimes involved in associations.

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