Lectin histochemistry of lipofuscin and certain ceroid pigments

1995 ◽  
Vol 103 (6) ◽  
pp. 435-445 ◽  
Author(s):  
Alberto J. Monserrat ◽  
Sebasti�n H. Benavides ◽  
Alejandro Berra ◽  
Silvia Fari�a ◽  
Silvia C. Vicario ◽  
...  
Keyword(s):  
Lectin Histochemistry

Characterization of the glycoconjugates of boar testis and epididymis

Reproduction ◽  
2000 ◽  
pp. 325-335 ◽  
Author(s):  
A Calvo ◽  
LM Pastor ◽  
S Bonet ◽  
E Pinart ◽  
M Ventura
Keyword(s):  
Ricinus Communis ◽  
Lectin Histochemistry ◽  
Apical Part ◽  
Seminiferous Tubules ◽  
In Situ Characterization ◽  
Intense Staining ◽  
Terminal Galactose ◽  
Boar Spermatozoa

Lectin histochemistry was used to perform in situ characterization of the glycoconjugates present in boar testis and epididymis. Thirteen horseradish peroxidase- or digoxigenin-labelled lectins were used in samples obtained from healthy fertile boars. The acrosomes of the spermatids were stained intensely by lectins with affinity for galactose and N-acetyl-galactosamine residues, these being soybean, peanut and Ricinus communis agglutinins. Sertoli cells were stained selectively by Maackia ammurensis agglutinin. The lamina propria of seminiferous tubules showed the most intense staining with fucose-binding lectins. The Golgi area and the apical part of the principal cells of the epididymis were stained intensely with many lectins and their distribution was similar in the three zones of the epididymis. On the basis of lectin affinity, both testis and epididymis appear to have N- and O-linked glycoconjugates. Spermatozoa from different epididymal regions showed different expression of terminal galactose and N-acetyl-galactosamine. Sialic acid (specifically alpha2,3 neuraminic-5 acid) was probably incorporated into spermatozoa along the extratesticular ducts. These findings indicate that the development and maturation of boar spermatozoa are accompanied by changes in glycoconjugates. As some lectins stain cellular or extracellular compartments specifically, these lectins could be useful markers in histopathological evaluation of diseases of boar testis and epididymis.

Interspecific variation of zona pellucida glycoconjugates in several species of marsupial

Reproduction ◽  
2000 ◽  
pp. 111-120 ◽  
Author(s):  
JA Chapman ◽  
OW Wiebkin ◽  
WG Breed
Keyword(s):  
Glycine Max ◽  
Sialic Acid ◽  
Zona Pellucida ◽  
Fluorescein Isothiocyanate ◽  
Interspecific Variation ◽  
Lectin Histochemistry ◽  
Sialic Acids ◽  
Arachis Hypogea ◽  
Marsupial Species ◽  
Similar Intensity

The zona pellucida glycoconjugate content of several marsupial species was investigated using differential lectin histochemistry. Ovaries from fat-tailed dunnarts, a southern brown bandicoot, grey short-tailed opossums, brushtail possums, ringtail possums, koalas and eastern grey kangaroos were fixed, embedded in paraffin wax, sectioned and stained with ten fluorescein isothiocyanate-conjugated lectins. Sections were also incubated with either neuraminidase or saponified, respectively, before incubation with the lectins to identify saccharide residues masked by sialic acids or O-acetyl groups on sialic acids. The zonae pellucidae surrounding the oocytes of the marsupials demonstrated interspecific variation in glycoconjugate content, with mannose-containing glycoconjugates exhibiting the greatest variation. Some of the zona pellucida glycoconjugates of all species, except those of the opossums, were masked by sialic acid with an increase in fluorescence with lectins from Arachis hypogea (PNA), and Glycine max (SBA), after desialylation. The disaccharide beta-galactose(1-4)N-acetyl-D-glucosamine appeared to be conformationally masked by O-acetyl groups of sialic acids in the zonae pellucidae of all species, with an increase in fluorescence with the lectin from Erythrina cristagalli (ECA), after saponification. Similar intensity and localization of beta-(1-4)-N-acetyl-D-glucosamine, as shown by staining of the lectin from Triticum vulgaris (WGA), to the inner and outer regions of the zona pellucida, were found to those reported in eutherian species. WGA fluorescence became uniform throughout the zonae pellucidae after saponification, indicating differential O-acetylation of sialic acids on the internal compartment of the zonae pellucidae.

scholarly journals Characterization of glycoconjugates in the intestinal mucosa of vertebrates by means of lectin histochemistry.

1989 ◽  
Vol 22 (1) ◽  
pp. 1-14 ◽  
Author(s):  
J. F. MADRID ◽  
J. BALLESTA ◽  
M. T. CASTELLS ◽  
J. A. MARIN ◽  
L. M. PASTOR
Keyword(s):  
Intestinal Mucosa ◽  
Lectin Histochemistry

Human and canine fucosidosis: a comparative lectin histochemistry study

1985 ◽  
Vol 67 (3-4) ◽  
pp. 265-271 ◽  
Author(s):  
J. Alroy ◽  
A. A. Ucci ◽  
C. D. Warren
Keyword(s):  
Lectin Histochemistry

Detection of glycosidic residues in carpal glands of wild and domestic pig revealed by basic and lectin histochemistry

1999 ◽  
Vol 181 (3) ◽  
pp. 269-274 ◽  
Author(s):  
V. Pedini ◽  
P. Scocco ◽  
C. Dall'Aglio ◽  
A.M. Gargiulo
Keyword(s):  
Lectin Histochemistry ◽  
Domestic Pig

scholarly journals Lectin histochemistry of the human testis

1998 ◽  
Vol 21 (6) ◽  
pp. 332-342 ◽  
Author(s):  
ARENAS ◽  
MADRID ◽  
BETHENCOURT ◽  
FRAILE ◽  
PANIAGUA
Keyword(s):  
Lectin Histochemistry ◽  
Human Testis
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