Qualitative and quantitative detection of alkaline phosphatase coupled to an oligonucleotide probe for somatostatin mRNA after in situ hybridization using unfixed rat brain tissue

1995 ◽  
Vol 103 (6) ◽  
pp. 463-471 ◽  
Author(s):  
Esther Asan ◽  
Peter Kugler
Keyword(s):  
Alkaline Phosphatase ◽  
In Situ Hybridization ◽  
Rat Brain ◽  
Brain Tissue ◽  
Oligonucleotide Probe ◽  
Quantitative Detection ◽  
Qualitative And Quantitative ◽  
Rat Brain Tissue

In situ hybridization histochemistry: localisation of vasopressin mRNA in rat brain using a biotinylated oligonucleotide probe

1988 ◽  
Vol 4 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Heii Arai ◽  
Piers C. Emson ◽  
Sudhir Agrawal ◽  
Chris Christodoulou ◽  
Michael J. Gait
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Oligonucleotide Probe ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry

scholarly journals MALDI Imaging and in Situ Identification of Integral Membrane Proteins from Rat Brain Tissue Sections

2013 ◽  
Vol 85 (15) ◽  
pp. 7191-7196 ◽  
Author(s):  
Joshua J. Nicklay ◽  
Glenn A. Harris ◽  
Kevin L. Schey ◽  
Richard M. Caprioli
Keyword(s):  
Membrane Proteins ◽  
Rat Brain ◽  
Brain Tissue ◽  
Integral Membrane Proteins ◽  
Maldi Imaging ◽  
Tissue Sections ◽  
Rat Brain Tissue

Liquid Chromatography Analysis of Clozapine in Rat Brain Tissue, Using its Molecularly Imprinted Polymer after Administration of Toxic Dose of Drug and Lipid Emulsion Therapy

2019 ◽  
Vol 15 (3) ◽  
pp. 251-257
Author(s):  
Bahareh Sadat Yousefsani ◽  
Seyed Ahmad Mohajeri ◽  
Mohammad Moshiri ◽  
Hossein Hosseinzadeh
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Molecularly Imprinted Polymer ◽  
Lipid Emulsion ◽  
Limit Of Detection ◽  
Imprinted Polymer ◽  
Toxic Dose ◽  
Molecularly Imprinted ◽  
The Brain ◽  
Rat Brain Tissue

Background:Molecularly imprinted polymers (MIPs) are synthetic polymers that have a selective site for a given analyte, or a group of structurally related compounds, that make them ideal polymers to be used in separation processes.Objective:An optimized molecularly imprinted polymer was selected and applied for selective extraction and analysis of clozapine in rat brain tissue.Methods:A molecularly imprinted solid-phase extraction (MISPE) method was developed for preconcentration and cleanup of clozapine in rat brain samples before HPLC-UV analysis. The extraction and analytical process was calibrated in the range of 0.025-100 ppm. Clozapine recovery in this MISPE process was calculated between 99.40 and 102.96%. The limit of detection (LOD) and the limit of quantification (LOQ) of the assay were 0.003 and 0.025 ppm, respectively. Intra-day precision values for clozapine concentrations of 0.125 and 0.025 ppm were 5.30 and 3.55%, whereas inter-day precision values of these concentrations were 9.23 and 6.15%, respectively. In this study, the effect of lipid emulsion infusion in reducing the brain concentration of drug was also evaluated.Results:The data indicated that calibrated method was successfully applied for the analysis of clozapine in the real rat brain samples after administration of a toxic dose to animal. Finally, the efficacy of lipid emulsion therapy in reducing the brain tissue concentration of clozapine after toxic administration of drug was determined.Conclusion:The proposed MISPE method could be applied in the extraction and preconcentration before HPLC-UV analysis of clozapine in rat brain tissue.

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