Genetic mechanisms of antigenic variation in infectious bursal disease virus: Analysis of a naturally occurring variant virus

Virus Genes ◽  
1992 ◽  
Vol 6 (3) ◽  
pp. 247-259 ◽  
Author(s):  
Dolores P. Lana ◽  
Christopher E. Beisel ◽  
Robert F. Silva
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Antigenic Variation ◽  
Infectious Bursal Disease ◽  
Naturally Occurring ◽  
Variant Virus ◽  
Bursal Disease ◽  
Genetic Mechanisms

Molecular basis of antigenic variation in infectious bursal disease virus

1994 ◽  
Vol 31 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Vikram N. Vakharia ◽  
Junkun He ◽  
Basheer Ahamed ◽  
David B. Snyder
Keyword(s):  
Disease Virus ◽  
Molecular Basis ◽  
Infectious Bursal Disease Virus ◽  
Antigenic Variation ◽  
Infectious Bursal Disease ◽  
Bursal Disease

scholarly journals Very virulent infectious bursal disease virus: reduced pathogenicity in a rare natural segment-B-reassorted isolate

2006 ◽  
Vol 87 (1) ◽  
pp. 209-216 ◽  
Author(s):  
Cyril Le Nouën ◽  
Gaëlle Rivallan ◽  
Didier Toquin ◽  
Pierre Darlu ◽  
Yannick Morin ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Virus Production ◽  
Infectious Bursal Disease ◽  
Naturally Occurring ◽  
Significant Difference ◽  
Bursal Disease ◽  
Difference Test ◽  
Post Infectious

The purpose of this study was to compare the molecular epidemiology of infectious bursal disease virus (IBDV) segments A and B of 50 natural or vaccine IBDV strains that were isolated or produced between 1972 and 2002 in 17 countries from four continents, with phenotypes ranging from attenuated to very virulent (vv). These strains were subjected to sequence and phylogenetic analysis based on partial sequences of genome segments A and B. Although there is co-evolution of the two genome segments (70 % of strains kept the same genetic relatives in the segment A- and B-defined consensus trees), several strains (26 %) were identified with the incongruence length difference test as exhibiting a significantly different phylogenetic relationship depending on which segment was analysed. This suggested that natural reassortment could have occurred. One of the possible naturally occurring reassortant strains, which exhibited a segment A related to the vvIBDV cluster whereas its segment B was not, was thoroughly sequenced (coding sequence of both segments) and submitted to a standardized experimental characterization of its acute pathogenicity. This strain induced significantly less mortality than typical vvIBDVs; however, the mechanisms for this reduced pathogenicity remain unknown, as no significant difference in the bursal lesions, post-infectious antibody response or virus production in the bursa was observed in challenged chickens.

Naturally occurring cell-adapted classic strain of infectious bursal disease virus

2020 ◽  
Vol 243 ◽  
pp. 108620 ◽  
Author(s):  
Yulong Wang ◽  
Linjin Fan ◽  
Nan Jiang ◽  
Li Gao ◽  
Kai Li ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Naturally Occurring ◽  
Bursal Disease

Naturally occurring reassortant infectious bursal disease virus in northern China

2015 ◽  
Vol 203 ◽  
pp. 92-95 ◽  
Author(s):  
Zhen Lu ◽  
Lizhou Zhang ◽  
Nian Wang ◽  
Yuming Chen ◽  
Li Gao ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Northern China ◽  
Infectious Bursal Disease ◽  
Naturally Occurring ◽  
Bursal Disease

scholarly journals Development of a Viral-Like Particle Candidate Vaccine Against Novel Variant Infectious Bursal Disease Virus

Vaccines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 142
Author(s):  
Yulong Wang ◽  
Nan Jiang ◽  
Linjin Fan ◽  
Li Gao ◽  
Kai Li ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Protective Efficacy ◽  
Expression System ◽  
Size Exclusion ◽  
Candidate Vaccine ◽  
Infectious Bursal Disease ◽  
Immune Protection ◽  
Bursal Disease ◽  
Novel Variant

Infectious bursal disease (IBD), an immunosuppressive disease of young chickens, is caused by infectious bursal disease virus (IBDV). Novel variant IBDV (nVarIBDV), a virus that can evade immune protection against very virulent IBDV (vvIBDV), is becoming a threat to the poultry industry. Therefore, nVarIBDV-specific vaccine is much needed for nVarIBDV control. In this study, the VP2 protein of SHG19 (a representative strain of nVarIBDV) was successfully expressed using an Escherichia coli expression system and further purified via ammonium sulfate precipitation and size-exclusion chromatography. The purified protein SHG19-VP2-466 could self-assemble into 25-nm virus-like particle (VLP). Subsequently, the immunogenicity and protective effect of the SHG19-VLP vaccine were evaluated using animal experiments, which indicated that the SHG19-VLP vaccine elicited neutralization antibodies and provided 100% protection against the nVarIBDV. Furthermore, the protective efficacy of the SHG19-VLP vaccine against the vvIBDV was evaluated. Although the SHG19-VLP vaccine induced a comparatively lower vvIBDV-specific neutralization antibody titer, it provided good protection against the lethal vvIBDV. In summary, the SHG19-VLP candidate vaccine could provide complete immune protection against the homologous nVarIBDV as well as the heterologous vvIBDV. This study is of significance to the comprehensive prevention and control of the recent atypical IBD epidemic.

scholarly journals Chicken Heat Shock Protein 70 Is an Essential Host Protein for Infectious Bursal Disease Virus Infection In Vitro

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 664
Author(s):  
Yufang Meng ◽  
Xiaoxue Yu ◽  
Chunxue You ◽  
Wenjuan Zhang ◽  
Yingfeng Sun ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Disease Virus ◽  
Heat Shock Protein 70 ◽  
Infectious Bursal Disease Virus ◽  
Host Protein ◽  
Infectious Bursal Disease ◽  
Economic Losses ◽  
Small Interfering Rnas ◽  
Bursal Disease

Infectious bursal disease virus (IBDV) infection causes pathogenicity and mortality in chickens, leading to huge economic losses in the poultry industry worldwide. Studies of host-virus interaction can help us to better understand the viral pathogenicity. As a highly conservative host factor, heat shock protein 70 (Hsp70) is observed to be involved in numerous viral infections. However, there is little information about the role of chicken Hsp70 (cHsp70) in IBDV infection. In the present study, the increased expression of cHsp70 was observed during IBDV-infected DF-1 cells. Further studies revealed that Hsp70 had similar locations with the viral double-stranded RNA (dsRNA), and the result of pull-down assay showed the direct interaction between cHsp70 with dsRNA, viral proteins (vp)2 and 3, indicating that maybe cHsp70 participates in the formation of the replication and transcription complex. Furthermore, overexpression of cHsp70 promoted IBDV production and knockdown of cHsp70 using small interfering RNAs (siRNA) and reducedviral production, implying the necessity of cHsp70 in IBDV infection. These results reveal that cHsp70 is essential for IBDV infection in DF-1 cells, suggesting that targeting cHsp70 may be applied as an antiviral strategy.

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