Yeast extract as a supplement to chemically defined medium for axenic culture ofCaenorhabditis briggsae

1969 ◽  
Vol 25 (6) ◽  
pp. 656-656 ◽  
Author(s):  
E. J. Buecher ◽  
E. L. Hansen
Keyword(s):  
Yeast Extract ◽  
Axenic Culture ◽  
Defined Medium ◽  
Chemically Defined Medium

Growth and hemolysin production by Haemophilus pleuropneumoniae cultivated in a chemically defined medium

1986 ◽  
Vol 32 (10) ◽  
pp. 801-805 ◽  
Author(s):  
James R. Maudsley ◽  
Solomon Kadis
Keyword(s):  
Growth Rate ◽  
Yeast Extract ◽  
Hemolytic Activity ◽  
Culture Supernatant ◽  
Iron Concentration ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Sterile Cell ◽  
Stages Of Growth ◽  
Cell Free Culture Supernatant

A chemically defined medium (CDM) has been developed which supports both growth and hemolysin production by Haemophilus pleuropneumoniae. Although the growth rate in stationary cultures was substantially slower in CDM than in trypticase soy broth plus 0.6% yeast extract (TSBYE) and slightly slower than in heart infusion broth (HIB), extracellular hemolysin activity in CDM was slightly higher than in HIB and 16-fold greater than in TSBYE. Maximum hemolytic activity was produced in CDM in early to mid log phase of growth. Hemolytic activity in sterile, cell-free culture supernatant fluids persisted for over 10 days at 4 °C and 3–5 days at 37 °C, but was completely destroyed at 56 °C after 30 min. Total hemolysin inactivation was also achieved in the presence of trypsin or pronase (10 units/mL), but no decrease in hemolytic activity was noted in the presence of DNase or RNase. Iron had little effect on the hemolytic activity in the early stages of growth. However, in the later stages of growth, iron had a pronounced effect with hemolyic activity decreasing as the iron concentration increased from 1 to 500 μM. None of these iron concentrations had any effect on the hemolytic activity when added directly to prepared cell-free culture supernatant fluids. The extracellur hemolysin produced by H. pleuropneumoniae in CDM appears to be a heat-labile protein the activity of which is influenced by iron at certain phases of growth.

Physiological ecology of the bloom-forming alga Chrysochromulina breviturrita (Prymnesiophyceae) from lakes influenced by acid precipitation

1985 ◽  
Vol 63 (12) ◽  
pp. 2231-2239 ◽  
Author(s):  
John D. Wehr ◽  
Lewis M. Brown ◽  
Kathryn O'Grady
Keyword(s):  
Axenic Culture ◽  
Lake Ontario ◽  
Acid Precipitation ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Tolerance Range ◽  
Northeastern North America ◽  
Ph Tolerance ◽  
Average Water ◽  
Nutritional Studies

Many soft-water lakes in northeastern North America subject to acid precipitation have been affected by lakewide odours. These odours are associated with blooms consisting of nearly unialgal populations of the greenish gold alga Chrysochromulina breviturrita Nich. (Prymnesiophyceae). The alga was isolated from Cinder Lake (Ontario) into axenic culture and maintained in a chemically defined medium which corresponds to the chemistry of lakes influenced by acid precipitation. The medium which was developed, Muskoka No. 42, is described in detail and compared with the average water chemistry of affected lakes. The pH tolerance range of C. breviturrita was determined to be 4.0–6.9, with an optimum of 5.5–6.9; the alga was unable to survive above neutrality. Additions of [Formula: see text] at 100 μM or greater completely inhibited growth. Nutritional studies suggested an inability to grow on any nitrogen source other than [Formula: see text]. The alga was also found to require the vitamins B12 and thiamine but not biotin. The present results represent the first published study in which a freshwater member of the Prymnesiophyceae has been maintained axenically in a defined medium.

Axenic Culture of Trypanosoma cruzi in a Chemically Defined Medium

1975 ◽  
Vol 61 (1) ◽  
pp. 144 ◽  
Author(s):  
Scoff J. Anderson ◽  
Stuart M. Krassner
Keyword(s):  
Trypanosoma Cruzi ◽  
Axenic Culture ◽  
Defined Medium ◽  
Chemically Defined Medium

scholarly journals Revealing nutritional requirements of MICP-relevant Sporosarcina pasteurii DSM33 for growth improvement in chemically defined and complex media

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Frédéric M. Lapierre ◽  
Jakob Schmid ◽  
Benjamin Ederer ◽  
Nina Ihling ◽  
Jochen Büchs ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Oxygen Transfer Rate ◽  
Carbon Sources ◽  
Construction Materials ◽  
Defined Medium ◽  
Nutritional Requirements ◽  
Chemically Defined Medium ◽  
Complex Media ◽  
Sporosarcina Pasteurii ◽  
Medium Components

AbstractMicrobial induced calcite precipitation (MICP) based on ureolysis has a high potential for many applications, e.g. restoration of construction materials. The gram-positive bacterium Sporosarcina pasteurii is the most commonly used microorganism for MICP due to its high ureolytic activity. However, Sporosarcina pasteurii is so far cultivated almost exclusively in complex media, which only results in moderate biomass concentrations at the best. Cultivation of Sporosarcina pasteurii must be strongly improved in order to make technological application of MICP economically feasible. The growth of Sporosarcina pasteurii DSM 33 was boosted by detecting auxotrophic deficiencies (L-methionine, L-cysteine, thiamine, nicotinic acid), nutritional requirements (phosphate, trace elements) and useful carbon sources (glucose, maltose, lactose, fructose, sucrose, acetate, L-proline, L-alanine). These were determined by microplate cultivations with online monitoring of biomass in a chemically defined medium and systematically omitting or substituting medium components. Persisting growth limitations were also detected, allowing further improvement of the chemically defined medium by the addition of glutamate group amino acids. Common complex media based on peptone and yeast extract were supplemented based on these findings. Optical density at the end of each cultivation of the improved peptone and yeast extract media roughly increased fivefold respectively. A maximum OD600 of 26.6 ± 0.7 (CDW: 17.1 ± 0.5 g/L) was reached with the improved yeast extract medium. Finally, culture performance and media improvement was analysed by measuring the oxygen transfer rate as well as the backscatter during shake flask cultivation.

Chemically-defined medium for growth and differentiation of mixed epithelial and connective tissues in organ culture

In Vitro ◽  
1976 ◽  
Vol 12 (6) ◽  
pp. 450-459 ◽  
Author(s):  
Gisele M. Hodges ◽  
Anthony H. Melcher
Keyword(s):  
Organ Culture ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Connective Tissues

Improved cryopreservation of domestic cat sperm in a chemically defined medium

2012 ◽  
Vol 78 (9) ◽  
pp. 2120-2128 ◽  
Author(s):  
M.M. Vick ◽  
H.L. Bateman ◽  
C.A. Lambo ◽  
W.F. Swanson
Keyword(s):  
Defined Medium ◽  
Domestic Cat ◽  
Chemically Defined Medium

A chemically defined medium for rearingEpiphyas postvittana(Lepidoptera: Tortricidae)

1974 ◽  
Vol 1 (2) ◽  
pp. 241-243 ◽  
Author(s):  
Pritam Singh
Keyword(s):  
Defined Medium ◽  
Chemically Defined Medium

scholarly journals Helicobacter pylori Growth and Urease Detection in the Chemically Defined Medium Ham's F-12 Nutrient Mixture

2001 ◽  
Vol 39 (11) ◽  
pp. 3842-3850 ◽  
Author(s):  
T. L. Testerman ◽  
D. J. McGee ◽  
H. L. T. Mobley
Keyword(s):  
Helicobacter Pylori ◽  
Defined Medium ◽  
Chemically Defined Medium
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