Inhibition of DNA synthesis and cell division by actinomycin D

1966 ◽  
Vol 22 (5) ◽  
pp. 294-295 ◽  
Author(s):  
H. Rothstein ◽  
J. Fortin ◽  
D. Sonneborn
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Actinomycin D ◽  
Inhibition Of Dna Synthesis

Inhibition of DNA synthesis and cell division by a cell surface sialoglycopeptide

1989 ◽  
Vol 139 (2) ◽  
pp. 269-274 ◽  
Author(s):  
Heideh Fattaey ◽  
Terry C. Johnson ◽  
Hsin-Hwei Chou
Keyword(s):  
Cell Division ◽  
Cell Surface ◽  
Dna Synthesis ◽  
A Cell ◽  
Inhibition Of Dna Synthesis

Interferon inhibition of DNA synthesis and cell division

1972 ◽  
Vol 18 (2) ◽  
pp. 145-151 ◽  
Author(s):  
M. V. O'Shaughnessy ◽  
S. H. S. Lee ◽  
K. R. Rozee
Keyword(s):  
Cell Division ◽  
Antiviral Activity ◽  
Dna Synthesis ◽  
S Phase ◽  
Cell Suspensions ◽  
Depression Effect ◽  
Growth Depression ◽  
Synchronized Cells ◽  
Inhibition Of Dna Synthesis ◽  
New Protein

Using monodispersed cell suspensions, interferon preparations were shown to have both a lethal and a growth-depression effect in the same concentration range as that required for antiviral activity. In addition, synchronized cells treated with interferon respond by delaying their normal uptake of thymidine during S phase until after a period during which new protein is synthesized. Puromycin added during this period prevents both the synthesis of this protein and the subsequent synthesis of DNA.

scholarly journals Effect of inhibition of DNA synthesis on u.v. sensitive Bs strains ofEscherichia coli

1969 ◽  
Vol 14 (2) ◽  
pp. 111-119 ◽  
Author(s):  
Michael H. L. Green ◽  
John Donch ◽  
Young S. Chung ◽  
Joseph Greenberg
Keyword(s):  
Escherichia Coli ◽  
Cell Division ◽  
Dna Synthesis ◽  
Nalidixic Acid ◽  
Specific Inhibitor ◽  
Ofescherichia Coli ◽  
Inhibition Of Dna Synthesis ◽  
Division Mechanism

The effect of nalidixic acid, a specific inhibitor of DNA synthesis, onEscherichia colistrain B (lon) and its u.v.-sensitive derivatives is examined. Strain B itself is sensitive to nalidixic acid, whereas its u.v.-resistant derivative B/r is resistant.It is shown that in allexr Astrains, in which u.v.-induced filamentation is suppressed, resistance to nalidixic acid is increased. Amongexr Astrains, Bs4 is exceptionally resistant to nalidixic acid. This is because nalidixic acid kills only growing cells and strain Bs4, atryauxotroph, may grow poorly under the conditions used to test nalidixic acid.Theuvrgenes of the HCR strains Bs1, Bs8 and Bs12 do not suppress u.v.-induced filamentation nor do they affect the response to nalidixic acid. Theuvrgene of strain Bs3 is unusual in increasing the tendency to filament and also sensitivity to nalidixic acid.Strains Bs1, Bs3 and Bs8 are all doubly mutated from strain B, the second mutation (notuvr) being responsible for their increased resistance to nalidixic acid as well as partially or completely suppressing filamentation.It is concluded that the cell division mechanism of (lon) strain B is sensitive to inhibition of DNA synthesis. Mutations which suppress the tendency of strain B to filament reduce its sensitivity to inhibition of DNA synthesis.

Inhibition of DNA synthesis and cell division in Salmonella typhimurium by azide

1974 ◽  
Vol 135 (4) ◽  
pp. 339-348 ◽  
Author(s):  
Z. Cieśla ◽  
Krystyna Mardarowicz ◽  
T. Klopotowski
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Salmonella Typhimurium ◽  
Inhibition Of Dna Synthesis

On the mechanism of 5-bromouracil inhibition of DNA synthesis and cell division

1968 ◽  
Vol 53 (2-3) ◽  
pp. 506-518 ◽  
Author(s):  
A. Toliver ◽  
E.H. Simon ◽  
P.T. Gilham
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Inhibition Of Dna Synthesis

scholarly journals CYTOLOGICAL STUDIES ON THE ANTIMETABOLITE ACTION OF 2,6-DIAMINOPURINE IN VICIA FABA ROOTS

1955 ◽  
Vol 1 (5) ◽  
pp. 399-419 ◽  
Author(s):  
George Setterfield ◽  
Robert E. Duncan
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Vicia Faba ◽  
Dna Content ◽  
Acid Synthesis ◽  
Nucleotide Metabolism ◽  
Cell Enlargement ◽  
Inhibition Of Dna Synthesis ◽  
Direct Incorporation ◽  
Inhibition Index

At a concentration of 9.6 x 10–5 M, 2,6-diaminopurine (DAP) completely inhibited cell enlargement, cell division, and DNA synthesis (determined by microphotometric measurement of Feulgen dye) in Vicia faba roots. Inhibition of cell enlargement was partially reversed by adenine, guanine, xanthine, adenosine, and desoxyadenosine. Guanine and the nucleosides gave the greatest reversal, suggesting that one point of DAP action upon cell enlargement is a disruption of nucleoside or nucleotide metabolism, possibly during pentosenucleic acid synthesis. DAP inhibited cell division by preventing onset of prophase. At the concentrations used it had no significant effect on the rate or appearance of mitoses in progress. Inhibition of entrance into prophase was not directly due to inhibition of DNA synthesis since approximately half of the inhibited nuclei had the doubled (4C) amount of DNA. Adenine competitively reversed DAP inhibition of cell division, giving an inhibition index of about 0.5. Guanine gave a slight reversal while xanthine, hypoxanthine, adenosine, and desoxyadenosine were inactive. A basic need for free adenine for the onset of mitosis was suggested by this reversal pattern. Meristems treated with DAP contained almost no nuclei with intermediate amounts of DNA, indicating that DAP prevented the onset of DNA synthesis while allowing that underway to reach completion. The inhibition of DNA synthesis was reversed by adenine, adenosine, and desoxyadenosine although synthesis appeared to proceed at a slower rate in reversals than in controls. Inhibition of DNA synthesis by DAP is probably through nucleoside or nucleotide metabolism. A small general depression of DNA content of nuclei in the reversal treatments was observed. This deviation from DNA "constancy" cannot be adequately explained at present although it may be a result of direct incorporation of DAP into DNA. The possible purine precursor, 4-amino-5-imidazolecarboxamide gave no reversal of DAP inhibition of cell elongation and cell division and only a slight possible reversal of inhibition of DNA synthesis.

Delayed inhibition of DNA synthesis in mouse jejunum by low doses of Actinomycin D

1966 ◽  
Vol 68 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Renato Baserga ◽  
Richard D. Estensen ◽  
Robert O. Petersen
Keyword(s):  
Dna Synthesis ◽  
Actinomycin D ◽  
Low Doses ◽  
Inhibition Of Dna Synthesis

Inhibition of DNA synthesis by actinomycin D and cycloheximide in synchronized HeLa cells

1968 ◽  
Vol 53 (2-3) ◽  
pp. 478-487 ◽  
Author(s):  
J.H. Kim ◽  
A.S. Gelbard ◽  
A.G. Perez
Keyword(s):  
Dna Synthesis ◽  
Hela Cells ◽  
Actinomycin D ◽  
Inhibition Of Dna Synthesis

scholarly journals RELATIONSHIPS BETWEEN NUCLEIC ACID SYNTHETIC PATTERNS AND ENCYSTMENT IN AGING UNAGITATED CULTURES OF ACANTHAMOEBA CASTELLANII

1971 ◽  
Vol 49 (2) ◽  
pp. 498-506 ◽  
Author(s):  
V. L. Rudick
Keyword(s):  
Nucleic Acid ◽  
Dna Synthesis ◽  
Dna Content ◽  
Rna Synthesis ◽  
Actinomycin D ◽  
Acanthamoeba Castellanii ◽  
Disc Electrophoresis ◽  
Dna And Rna ◽  
Inhibition Of Dna Synthesis ◽  
Growth Deceleration

Changes in the levels of DNA and RNA syntheses have been studied in unagitated cultures of Acanthamoeba castellanii during the phases of logarithmic multiplication (LM) and population growth deceleration (PGD). Pulse-labeling experiments show that the rate of DNA synthesis decreases at the same time that DNA per cell is known to drop by 50%. The drop in DNA content has been explained by demonstrating with hydroxyurea that the majority of LM amebas can replicate once when DNA synthesis is inhibited and, therefore, must be in G2, whereas the PGD amebas cannot multiply in the presence of inhibitor and, therefore, must be in G1. The inhibition of DNA synthesis in LM or PGD cells has been shown to induce encystment. The rate of RNA synthesis, as illustrated by pulse-labeling experiments, increases 25% in late LM-early PGD while RNA per cell increases 75%. The rate of synthesis then decreases 65%. The majority of accumulated RNA has been demonstrated to be ribosomal by disc electrophoresis. By using actinomycin D at different stages during the RNA build-up, the ability of the amebas to encyst has been shown to depend on the presence of this RNA. The observations on DNA and RNA are discussed with respect to the occurrence of cysts in the cultures during PGD.

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