Relating Logic and Relating Semantics. History, Philosophical Applications and Some of Technical Problems

Here, we discuss historical, philosophical and technical problems associated with relating logic and relating semantics. To do so, we proceed in three steps. First, Section 1 is devoted to providing an introduction to both relating logic and relating semantics. Second, we address the history of relating semantics and some of the main research directions and their philosophical applications. Third, we discuss some technical problems related to relating semantics, particularly whether the direct incorporation of the relation into the language of relating logic is needed. The starting point for our considerations presented here is the 1st Workshop On Relating Logic and the selected papers for this issue.

Shelf-stable electrophilic reagents for the direct incorporation of SCF2CF2H and SCF2CF3 motifs

The introduction of fluoroalkylthioether groups has attracted the attention of the drug discovery community given the special physicochemical and pharmacokinetic features they confer to bioactive compounds. Synthetic advances in the field have been capitalized by methods to incorporate SCF3 and SCF2H motifs, however, longer and synthetically more challenging polyfluoroethyl chains are still underdeveloped. Here, two saccharin-based electrophilic reagents have been disclosed for the efficient incorporation of SCF2CF2H and SCF2CF3 motifs. Their reactivity performance has been thoroughly investigated with a variety of nucleophiles such as thiols, alcohols, amines, alkenes, (hetero)aromatics, and organometallic species, including natural products and pharmaceuticals. Finally, multigram-scale preparation and divergent derivatization has been explored from SCF2CF2H derivatives.

An Ethnographic Study on the Non-Material Impacts of the Educated Youth Program

In a movement that led to over a million civilian deaths and the persecution of millions more, the Chinese Cultural Revolution mobilized all aspects of state in order to upheave traditional hierarchy and maintain a Maoist regime. During this time, under the Educated Youth Program, nearly 17 million students of varying societal backgrounds moved to rural locations to develop “proper” values and skills. Through a qualitative ethnographic case study, twelve narrative interviews conducted with participants of the program, representing multiple regions in China, were examined under the principles of thematic analysis in order to discover overarching themes about the immaterial impacts that occurred as a result of this wide-scale emigration and whether these changes were positive or negative. Data indicated that (1) because of the rural environment and hard-working people around them, many participants gained perseverance, and (2) increased interactions with those of ethnic and economic different backgrounds led to greater acceptance of others. The findings of the study suggest that the Educated Youth Program was ultimately beneficial for its participants in regards to their overall life. These results help build a more comprehensive understanding about the impacts of rural education in China through the direct incorporation of participants’ perspectives.

Incorporating the Relation into the Language?

In this paper we discuss whether the relation between formulas in the relating model can be directly introduced into the language of relating logic, and present some stances on that problem. Other questions in the vicinity, such as what kind of functor would be the incorporated relation, or whether the direct incorporation of the relation into the language of relating logic is really needed, will also be addressed.

A Genetically Encoded Picolyl Azide for Improved Live Cell Copper Click Labeling

Bioorthogonal chemistry allows rapid and highly selective reactivity in biological environments. The copper-catalyzed azide–alkyne cycloaddition (CuAAC) is a classic bioorthogonal reaction routinely used to modify azides or alkynes that have been introduced into biomolecules. Amber suppression is an efficient method for incorporating such chemical handles into proteins on the ribosome, in which noncanonical amino acids (ncAAs) are site specifically introduced into the polypeptide in response to an amber (UAG) stop codon. A variety of ncAA structures containing azides or alkynes have been proven useful for performing CuAAC chemistry on proteins. To improve CuAAC efficiency, biologically incorporated alkyne groups can be reacted with azide substrates that contain copper-chelating groups. However, the direct incorporation of copper-chelating azides into proteins has not been explored. To remedy this, we prepared the ncAA paz-lysine (PazK), which contains a picolyl azide motif. We show that PazK is efficiently incorporated into proteins by amber suppression in mammalian cells. Furthermore, PazK-labeled proteins show improved reactivity with alkyne reagents in CuAAC.

From Headhunting to Head Taxes

This article investigates Dutch colonial practices on the Moluccan island of Seram in the late nineteenth and early twentieth centuries. Seram’s mountainous interior was the domain of ungoverned, peripatetic Alfurs who engaged in headhunting. For a long time, they were rendered untouched by colonialism and administered through coastal intermediaries. After 1900, renewed imperial-civilizational vigour demanded the direct incorporation and ‘civilization’ of Seram’s stateless spaces. A series of expeditions subjected the Alfurs to registration, categorization, and taxation, which this article argues were seen as pivotal, moralizing tools of colonial social-engineering, used to inscribe subjected people into the state and instil compliant and ‘productive’ behaviour. However, rather than a replacement of indigenous orders with European modernity, colonization produced a hybrid fusion of colonial strategies of domination with indigenous cultural practices of state-evasion. This article demonstrates that colonial governance was a site of interaction, in which colonial developmentalism and modernity were actively negotiated and challenged.

Direct C-H-Sulfonylation of 6-Membered Nitrogen-Heteroaromatics

Heterocyclic sulfones and sulfonamides represent important structural motives in medicinal chemistry and drug development. Therefore, efficient and reliable methods for their construction from simple building blocks are in high demand. Herein we report a novel approach for the direct C-H-sulfonylation of N heteroaromatics via N-activation with triflic anhydride (Tf2O), base-mediated addition of a sulfinate salt and subsequent rearomatization through trifluoromethanesulfinate elimination. This operationally simple one-pot protocol enables direct access to various sulfonylated 6-ring N-heterocycles. It is applicable to the late-stage functionalization of complex, drug-like molecules. The direct incorporation of sulfur dioxide with organometallic reagents as well as the utilization of a rongalite-based sulfonylation reagent provide opportunities for a highly modular synthesis of N-heterocyclic sulfones and sulfonamides from three different building blocks.

Yogurt Fortification by the Addition of Microencapsulated Stripped Weakfish (Cynoscion guatucupa) Protein Hydrolysate

The aim of the present work was to fortify yogurt by adding a stripped weakfish (Cynoscion guatucupa) protein hydrolysate obtained with the enzyme Protamex and microencapsulated by spray drying, using maltodextrin (MD) as wall material. The effects on the physicochemical properties, syneresis, texture, viscoelasticity, antioxidant and ACE inhibitory activities of yogurt after 1 and 7 days of storage were evaluated. In addition, microbiological and sensory analyses were performed. Four yogurt formulations were prepared: control yogurt (without additives, YC), yogurt with MD (2.1%, YMD), with the free hydrolysate (1.4%, YH) and the microencapsulated hydrolysate (3.5%, YHEn). Yogurts to which free and microencapsulated hydrolysates were added presented similar characteristics, such as a slight reduction in pH and increased acidity, with a greater tendency to present a yellow color compared with the control yogurt. Moreover, they showed less syneresis, the lowest value being that of YHEn, which also showed a slight increase in cohesiveness and greater rheological stability after one week of storage. All yogurts showed high counts of the microorganisms used as starters. The hydrolysate presence in both forms resulted in yogurts with antioxidant activity and potent ACE-inhibitory activity, which were maintained after 7 days of storage. The incorporation of the hydrolysate in the microencapsulated form presented greater advantages than the direct incorporation, since encapsulation masked the fishy flavor of the hydrolysate, resulting in stable and sensorily acceptable yogurts with antioxidant and ACE inhibitory activities.

Using a Quadruplet Codon to Expand the Genetic Code of an Animal

Genetic code expansion in multicellular organisms is currently limited to the use of repurposed amber stop codons. Here we introduce a system for the use of quadruplet codons to direct incorporation of non-canonical amino acids in vivo in an animal, the nematode worm Caenorhabditis elegans. We develop hybrid pyrrolysyl tRNA variants to incorporate non-canonical amino acids in response to the quadruplet codon UAGA. We demonstrate the efficiency of the quadruplet decoding system by incorporating photocaged amino acids into two proteins widely used as genetic tools. We use photocaged lysine to express photocaged Cre recombinase for the optical control of gene expression and photocaged cysteine to express photo-activatable caspase for light inducible cell ablation. Our approach will facilitate the routine adoption of quadruplet decoding for genetic code expansion in eukaryotic cells and multicellular organisms.