Effect of epidermal growth factor on growth and maturation of fetal and neonatal rat small intestine in organ culture

1986 ◽  
Vol 42 (8) ◽  
pp. 950-952 ◽  
Author(s):  
C. N. Conteas ◽  
J. M. DeMorrow ◽  
A. P. N. Majumdar
Keyword(s):  
Small Intestine ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Organ Culture ◽  
Neonatal Rat ◽  
Rat Small Intestine ◽  
Epidermal Growth

Specific receptors for epidermal growth factor in rat intestinal microvillus membranes

1988 ◽  
Vol 254 (3) ◽  
pp. G429-G435 ◽  
Author(s):  
J. F. Thompson
Keyword(s):  
Small Intestine ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Sodium Dodecyl ◽  
Intestinal Lumen ◽  
Scatchard Analysis ◽  
Rat Small Intestine ◽  
Binding Studies ◽  
Epidermal Growth ◽  
Specific Receptors

Epidermal growth factor (EGF) is present in high concentrations in milk, salivary, and pancreaticobiliary secretions. EGF, delivered to the intestinal lumen by these fluids, appears to influence intestinal proliferation. Because EGF exerts its mitogenic effect through binding to specific membrane-bound receptors, binding studies of 125I-labeled EGF to purified microvillus membrane (MVM) preparations from fetal, newborn, and adult rat small intestine were performed. Using the membrane filter technique, binding of 125I-EGF to adult MVM was specific, saturable, and reversible. Adult and fetal MVM binding was rapid and reached a plateau after 30 min at both 20 and 37 degrees C. No binding was detected at 4 degrees C. Specific binding increased linearly from 0 to 75 micrograms MVM protein. Scatchard analysis revealed a single class of receptors in fetal and adult MVM with an association constant of 1.0 +/- 0.35 X 10(9) and 2.3 +/- 1.6 X 10(9) M-1, respectively. Binding capacity was 435.0 +/- 89 and 97.7 +/- 41.3 fmol 125I-EGF bound/mg MVM protein for fetal and adult MVM, respectively. Newborn MVM binding was negligible. After binding, cross-linking utilizing disuccinimidyl suberate, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, autoradiography revealed a 170-kDa receptor. These data demonstrate specific receptors for EGF on MVM of rat small intestine and, thus, suggest a mechanism for the intraluminal regulation of enterocyte proliferation by EGF.

Epidermal growth factor reduces ischemia-reperfusion injury in rat small intestine

2002 ◽  
Vol 30 (7) ◽  
pp. 1576-1580 ◽  
Author(s):  
Xavier Villa ◽  
John W. Kuluz ◽  
Charles L. Schleien ◽  
John F. Thompson
Keyword(s):  
Small Intestine ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Rat Small Intestine ◽  
Epidermal Growth

The effect of epidermal growth factor on the incremental Young’s moduli in the rat small intestine

2003 ◽  
Vol 25 (5) ◽  
pp. 413-418 ◽  
Author(s):  
D. Liao ◽  
J. Yang ◽  
J. Zhao ◽  
Y. Zeng ◽  
L. Vinter-Jensen ◽  
...  
Keyword(s):  
Small Intestine ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Rat Small Intestine ◽  
Young’S Moduli ◽  
Epidermal Growth

scholarly journals The expression of epidermal growth factor and transforming growth factor-α mRNA in the small intestine of suckling rats: organ culture study

FEBS Letters ◽  
1998 ◽  
Vol 435 (1) ◽  
pp. 119-124 ◽  
Author(s):  
Bohuslav Dvořák ◽  
Jiřina Kolı́nská ◽  
Debra L McWilliam ◽  
Catherine S Williams ◽  
Travis Higdon ◽  
...  
Keyword(s):  
Small Intestine ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Organ Culture ◽  
Transforming Growth Factor ◽  
Culture Study ◽  
Suckling Rats ◽  
Transforming Growth Factor Α ◽  
Epidermal Growth ◽  
Factor Α

Epidermal Growth Factor as a Regulatory Hormone Maintaining a Low pH Microclimate in the Rat Small Intestine

1989 ◽  
Vol 78 (6) ◽  
pp. 457-459 ◽  
Author(s):  
Takafumi Iwatsubo ◽  
Masayo Yamazaki ◽  
Yuichi Sugiyama ◽  
Hiroshi Suzuki ◽  
Shigeo Yanai ◽  
...  
Keyword(s):  
Small Intestine ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Low Ph ◽  
Rat Small Intestine ◽  
Epidermal Growth

Is polyamine synthesis involved in the proliferative response of the intestinal epithelium to urogastrone-epidermal growth factor?

1989 ◽  
Vol 76 (6) ◽  
pp. 595-598 ◽  
Author(s):  
R. A. Goodlad ◽  
H. Gregory ◽  
N. A. Wright
Keyword(s):  
Cell Proliferation ◽  
Small Intestine ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Proliferative Response ◽  
Intestinal Cell ◽  
Intestinal Epithelial ◽  
Polyamine Synthesis ◽  
Proliferative Effect ◽  
Epidermal Growth

1. Intestinal epithelial cell proliferation was measured in rats maintained on total parenteral nutriton (TPN), in TPN rats given 300 μg of recombinant human epidermal growth factor (urogastrone-epidermal growth factor, URO-EGF) day−1 kg−1, and in further groups given URO-EGF and difluoromethylornithine (DFMO), an inhibitor of the enzyme ornithine decarboxylase (ODC). 2. URO-EGF significantly increased intestinal cell proliferation throughout the gastrointestinal tract. The proliferative response of the colon was particularly pronounced. 3. DFMO reduced the proliferative effect of urogastrone in the stomach and small intestine. DFMO also reduced URO-EGF-stimulated intestinal cell proliferation in the colon, but to a lesser extent. 4. It is concluded that ODC is essential for effecting the proliferative response of the stomach and small intestine to URO-EGF, but this role may be less important in the colon.

Epidermal growth factor reduces the development of necrotizing enterocolitis in a neonatal rat model

2002 ◽  
Vol 282 (1) ◽  
pp. G156-G164 ◽  
Author(s):  
Bohuslav Dvorak ◽  
Melissa D. Halpern ◽  
Hana Holubec ◽  
Catherine S. Williams ◽  
Debra L. McWilliam ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Mrna Expression ◽  
Necrotizing Enterocolitis ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
Gastrointestinal Disease ◽  
Neonatal Rat ◽  
Epidermal Growth ◽  
Neonatal Rat Model

Necrotizing enterocolitis (NEC) is the most common gastrointestinal disease of prematurely born infants. Maternal milk plays an important protective role against NEC development and is the major source of epidermal growth factor (EGF) for neonates. The aim of this study was to examine the effect of orally administered EGF on the incidence of NEC in a neonatal rat model. Newborn rats were artificially fed either with growth factor-free rat milk substitute (RMS) or RMS supplemented with 500 ng/ml of EGF (RMS+EGF). Experimental NEC was induced by exposure to asphyxia and cold stress. Development of NEC was evaluated by gross and histological scoring of damage in the ileum. Ileal EGF receptor (EGF-R), EGF, and transforming growth factor-α mRNA expression was assessed by RT competitive-PCR, and the EGF-R was localized by immunohistochemistry. EGF supplementation of formula reduced the incidence and severity of NEC in rats (13/16 RMS vs. 4/13 RMS+EGF). Ileal EGF-R mRNA expression was markedly increased in the RMS group compared with RMS+EGF. Enhanced EGF-R expression in the RMS group was localized predominantly in the epithelial cells of injured ileum. These data suggest a new potential therapeutic approach for the prevention and treatment of NEC.

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