Prolongation of the survival of skin grafts in mice by PUVA treatment

ABSTRACT The degree of genetic divergence which has occurred between a number of inbred strains of mice and between two sublines of inbred rats was assessed by determining the fate of inter-subline skin grafts. Sublines which had been separated for 29 and 42 generations possessed no detectable incompatibility, while three combinations of sublines judged to have been maintained apart for from 123 to 129 generations showed slight degrees of histoincompatibility. One pair of sublines which had been separated for 119 generations demonstrated a marked degree of incompatibility, and an F2 test suggested that mutations had occurred at four or five histocompatibility loci.

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Skin Grafts

Otolaryngologic Clinics of North America ◽

10.1016/s0030-6665(20)30714-3 ◽

1994 ◽

Vol 27 (1) ◽

pp. 25-37 ◽

Cited By ~ 8

Author(s):

Guy J. Petruzzelli ◽

Jonas T. Johnson

Keyword(s):

Skin Grafts

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The Advantage of the Supercooling Storage Method for Transplantable Sources: Human Umbilical Vessel Endothelial Cells and Mouse Skin Grafts

Transplantation Proceedings ◽

10.1016/j.transproceed.2021.03.042 ◽

2021 ◽

Author(s):

Mu-Young Kim ◽

Hun-Young Yoon ◽

Soojung Lee

Keyword(s):

Endothelial Cells ◽

Mouse Skin ◽

Skin Grafts ◽

Storage Method ◽

Umbilical Vessel

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Reducing alcohol and/or cocaine-induced reward and toxicity via an epidermal stem cell-based gene delivery platform

Molecular Psychiatry ◽

10.1038/s41380-021-01043-y ◽

2021 ◽

Author(s):

Qingyao Kong ◽

Yuanyuan Li ◽

Jiping Yue ◽

Xiaoyang Wu ◽

Ming Xu

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Gene Delivery ◽

Unmet Need ◽

Skin Grafts ◽

Epidermal Stem Cell ◽

Epidermal Stem Cells ◽

Concurrent Use ◽

Delivery Platform ◽

Glucagon Like Peptide 1

AbstractAlcohol use disorder (AUD) is one of the foremost public health problems. Alcohol is also frequently co-abused with cocaine. There is a huge unmet need for the treatment of AUD and/or cocaine co-abuse. We recently demonstrated that skin grafts generated from mouse epidermal stem cells that had been engineered by CRISPR-mediated genome editing could be transplanted onto mice as a gene delivery platform. Here, we show that expression of the glucagon-like peptide-1 (GLP1) gene delivered by epidermal stem cells attenuated development and reinstatement of alcohol-induced drug-taking and seeking as well as voluntary oral alcohol consumption. GLP1 derived from the skin grafts decreased alcohol-induced increase in dopamine levels in the nucleus accumbens. In exploring the potential of this platform in reducing concurrent use of drugs, we developed a novel co-grafting procedure for both modified human butyrylcholinesterase (hBChE)- and GLP1-expressing cells. Epidermal stem cell-derived hBChE and GLP1 reduced acquisition of drug-taking and toxicity induced by alcohol and cocaine co-administration. These results imply that cutaneous gene delivery through skin transplants may add a new option to treat drug abuse and co-abuse.

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Algorithm of rational approach to reconstruction in Fournier’s disease

Open Medicine ◽

10.1515/med-2021-0294 ◽

2021 ◽

Vol 16 (1) ◽

pp. 1028-1037

Author(s):

Domenico Tripodi ◽

Maria Ida Amabile ◽

Federica Gagliardi ◽

Federico Frusone ◽

Marzia Varanese ◽

...

Keyword(s):

Necrotizing Fasciitis ◽

Fournier’S Gangrene ◽

Rational Approach ◽

Skin Grafts ◽

Local Infection ◽

Fournier's Gangrene ◽

Rare Form ◽

Perineal Region ◽

Reconstructive Technique ◽

Diabetes And Obesity

Abstract Background Fournier’s gangrene is a rare form of necrotizing fasciitis that affects the genital area up to the perineal region and sometimes the abdominal wall. Objectives Our article aims to show that in the treatment of extensive forms of Fournier’s gangrene, correct use of flap and skin grafts and a quick reconstruction of the exposed tissues avoid scarring retraction of the testicles and deformation of the penis. Materials and methods We retrospectively reviewed the clinical and photographic data of Fournier’s gangrene cases treated at our Institute. The data were evaluated to obtain an estimate of the results of the reconstructive technique used, in terms of percentage of occurred healings and eventual complications. Results A total of 34 patients underwent surgery for Fournier’s gangrene. In nine cases (26.5%), we had minor complications: in four patients, suffering from diabetes and obesity, a retard in attachment of graft occurred, while in five patients with perianal problems there was a delay in healing due to the onset of local infection. Conclusion The reconstruction approach described here may reduce surgical times. In Fournier’s gangrene, the exposed tissues must be reconstructed as quickly as possible.

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