TRANSPLANTATION OF GOLD NANOPARTICLES WITH OR WITHOUT BONE MARROW STEM CELLS FOR REGENERATION OF SURGICALLY INDUCED TONGUE DEFECT IN RATS

The ultimate goal of tongue reconstruction is restoring the tongue integrity while preserve its critical functions(i.e., articulation, mastication, and deglutition) and minimize the morbidity profile of a selected reconstructive technique. Mesenchymal stem cells (MSCs) used in tissue engineering became a therapeutic selectiondue to numerous advantagesas regeneration of damaged tissues withhigh quality without the formation of fibrous tissue, minimal donor site morbidity in comparison withautografts and a low risk ofdisease transmition and autoimmune rejection. Bone marrow stem cells (BMSCs) areexpandable stem, self-renewing,embedded at the site of injury and motivate tissue regeneration and wound healing. In oral wounds, they revealgreater re-epithelialization, intracellular matrix formation,cellularity, and neoangiogenesis, thusspeed up wound healing.Gold nanoparticles (GNPs) application in medical fields is growingdue to the chemical and physical properties like biocompatibility, optical properties,facile surface modification and stability.GNP usage in regenerative medicine is safe if the implanted tissue is replacing a tissue/organresectedbecause of tumor.

Simultaneous Volar Pulp and Nail Bed Reconstruction with the Second Toe Onychocutaneous Free Flap

Fingertip injury is one of the most common hand injuries. Although several types of advancement and cross-finger flaps exist, they would not be essential for nail bed defects. The authors present a simultaneous volar pulp and nail bed reconstructive technique that uses a second toe onychocutaneous free flap. Four patients without amputees underwent fingertip amputation reconstruction between 2011 and 2019. After thorough debridement, the defect size was estimated, and the digital arteries, nerves, and veins of the recipient were evaluated. The flap, composed of pulp tissue and nail bed, was harvested with continuity from the second toe. Additional split-thickness skin grafts were performed in two cases. All flaps survived without considerable complications. We evaluated the scar and contour, and nail growth was reported over Zook’s criteria grade B. The second toe onychocutaneous free flap provides a reliable option for fingertip defects that involve pulp tissue and nail bed without further amputation.

146 Ligamentization and Remnant Integration: Implications for Scapholunate Reconstruction

Introduction Scapholunate interosseous ligament (SLIL) injuries are common but remain a therapeutic challenge. Current treatment modalities prioritize restoration of normal anatomy with reconstruction where appropriate. To date, no reconstructive technique has been described that discusses the potential benefit of the preservation of the scapholunate ligament remnant. Little is known about the ‘ligamentization’ of grafts within the wrist. However, a growing body of knee literature suggests that remnant sparing may confer some benefit. In the absence of wrist specific studies, this literature must guide areas for potential augmentation of current surgical practices. Method We conducted a systematic review of the literature using Pubmed, Embase and Medline. Keywords were ‘Ligament’ AND ‘Reconstruction’ AND ‘Remnant’. We identified 366 original studies for title and abstract review. A total of 5 studies were included in the final analysis. Results In 5 animal studies, remnant stump integration resulted in enhanced ligamentization of grafted tissue as measured by vascularity, biomechanics, and proprioception. Conclusions Our review demonstrates a trend towards enhanced early ligamentization in grafts that incorporate the remnant stump. Remnant preservation is not currently performed in SLIL reconstruction and may provide an avenue for enhanced therapies. As such, it is a promising area for further, wrist specific, research.

Algorithm of rational approach to reconstruction in Fournier’s disease

Background Fournier’s gangrene is a rare form of necrotizing fasciitis that affects the genital area up to the perineal region and sometimes the abdominal wall. Objectives Our article aims to show that in the treatment of extensive forms of Fournier’s gangrene, correct use of flap and skin grafts and a quick reconstruction of the exposed tissues avoid scarring retraction of the testicles and deformation of the penis. Materials and methods We retrospectively reviewed the clinical and photographic data of Fournier’s gangrene cases treated at our Institute. The data were evaluated to obtain an estimate of the results of the reconstructive technique used, in terms of percentage of occurred healings and eventual complications. Results A total of 34 patients underwent surgery for Fournier’s gangrene. In nine cases (26.5%), we had minor complications: in four patients, suffering from diabetes and obesity, a retard in attachment of graft occurred, while in five patients with perianal problems there was a delay in healing due to the onset of local infection. Conclusion The reconstruction approach described here may reduce surgical times. In Fournier’s gangrene, the exposed tissues must be reconstructed as quickly as possible.