The Advantage of the Supercooling Storage Method for Transplantable Sources: Human Umbilical Vessel Endothelial Cells and Mouse Skin Grafts

2021 ◽  
Author(s):  
Mu-Young Kim ◽  
Hun-Young Yoon ◽  
Soojung Lee
Keyword(s):  
Endothelial Cells ◽  
Mouse Skin ◽  
Skin Grafts ◽  
Storage Method ◽  
Umbilical Vessel

The cell type mediating hyperacute rejection of allogeneic mouse skin grafts

1977 ◽  
Vol 33 (1) ◽  
pp. 219-223 ◽  
Author(s):  
E.J. Eichwald ◽  
C.M. Craven ◽  
C. Spellman ◽  
M.L. Dolberg
Keyword(s):  
Mouse Skin ◽  
Hyperacute Rejection ◽  
Skin Grafts ◽  
Cell Type

Hemodynamic factor influencing morphology and stress fiber expression in rat umbilical vessel endothelial cells

Placenta ◽  
1996 ◽  
Vol 17 (8) ◽  
pp. A8-A8
Author(s):  
H ISHIKAWA ◽  
R SAWA ◽  
Y YONEYAMA ◽  
S SHIN ◽  
S KEIJI ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stress Fiber ◽  
Umbilical Vessel

A method of isolation and culture of microvascular endothelial cells from mouse skin

2005 ◽  
Vol 70 (3) ◽  
pp. 198-204 ◽  
Author(s):  
Sung Tae Cha ◽  
Dodanim Talavera ◽  
Erhan Demir ◽  
Anjali K. Nath ◽  
M. Rocio Sierra-Honigmann
Keyword(s):  
Endothelial Cells ◽  
Mouse Skin ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial

scholarly journals Pilot Study of the Biological Properties and Vascularization of 3D Printed Bilayer Skin Grafts

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Yige Huyan ◽  
Qin Lian ◽  
Tingze Zhao ◽  
Dichen Li ◽  
Jiankang He
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Double Layer ◽  
Dermal Fibroblasts ◽  
Microvascular Endothelial Cells ◽  
Human Dermal Fibroblasts ◽  
Skin Grafts ◽  
Full Thickness ◽  
3D Printed ◽  
Microvascular Endothelial

The skin is the largest human organ, and defects in the skin with a diameter greater than 4 cm do not heal without treatment. Allogeneic skin transplantation has been used to allow wound healing, but many grafts do not survive after implantation, due to multiple complications in the procedure. In the present study, the vascularization of three-dimensional (3D) printed full-thickness skin grafts was investigated. Dermal-epithelial grafts were transplanted into a nude mouse model to evaluate integration with the host tissue and the extent of wound healing. To create microvessels in the skin grafts, a bilayer structure consisting of human dermal fibroblasts, keratinocytes, and microvascular endothelial cells was designed and fabricated using an extruded 3D printer. Human dermal fibroblasts and human microvascular endothelial cells were mixed with gelatin-sodium alginate composite hydrogel as the dermis, and human keratinocytes were mixed with gel as the epithelium. Confocal imaging allowed visualization of the location of the cells in the double-layer skin grafts. A full-thickness wound was created on the backs of nude mice and then covered with a double-layer skin graft. Various groups of mice were tested. Animals were euthanized and tissue samples collected after specified time points. Compared with the control group, wound contraction improved by approximately 10%. Histological analysis demonstrated that the new skin had an appearance similar to that of normal skin and with a significant degree of angiogenesis. The results of the immunohistochemical analysis demonstrated that the transplanted cells survived and participated in the healing process.

scholarly journals Cartilage Oligometric Matrix Protein-Angiopoietin-1 Promotes Revascularization Through Increased Survivin Expression in Dermal Endothelial Cells of Skin Grafts in Mice

2007 ◽  
Vol 171 (5) ◽  
pp. 1682-1690 ◽  
Author(s):  
Seung-Jae Byun ◽  
Kyu-Sil Choi ◽  
Sung Hoon Park ◽  
Nam Woo Cho ◽  
Chang Hyun Yoo ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Matrix Protein ◽  
Survivin Expression ◽  
Skin Grafts ◽  
Angiopoietin 1

scholarly journals Expression of the CD34 gene in vascular endothelial cells

Blood ◽  
1990 ◽  
Vol 75 (12) ◽  
pp. 2417-2426 ◽  
Author(s):  
L Fina ◽  
HV Molgaard ◽  
D Robertson ◽  
NJ Bradley ◽  
P Monaghan ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelium ◽  
Umbilical Artery ◽  
Vascular Endothelial Cells ◽  
Umbilical Vein ◽  
Vascular Endothelial ◽  
Umbilical Vessel ◽  
Cd34 Antigen ◽  
Large Vessels ◽  
Hemopoietic Progenitor

Abstract All seven of a set of CD34 monoclonal antibodies that recognize epitopes on an approximately 110 Kd glycoprotein on human hemopoietic progenitor cells also bind to vascular endothelium. Capillaries of most tissues are CD34 positive, as are umbilical artery and, to a lesser extent, vein, but the endothelium of most large vessels and the endothelium of placental sinuses are not. Angioblastoma cells and parafollicular mesenchymal cells in fetal skin are also CD34 positive, as are some stromal elements. An approximately 110 Kd protein can be identified by Western blot analysis with CD34 antibodies in detergent extracts of freshly isolated umbilical vessel endothelial cells, and CD34 mRNA is present in cultured umbilical vein cells as well as other tissues rich in vascular endothelium (breast, placenta). These data indicate that the binding of CD34 antibodies to vascular endothelium is to the CD34 gene product, and not to crossreactive epitopes. Despite the presence of CD34 mRNA in cultured, proliferating endothelial cells, the latter do not bind CD34 antibodies. In addition, CD34 antigen cannot be upregulated by growth factors. We conclude that under these conditions, CD34 protein is downregulated or processed into another form that is unreactive with CD34 antibodies. Electron microscopy of umbilical artery, breast, and kidney capillary vessels reveals that in all three sites, CD34 molecules are concentrated on membrane processes, many of which interdigitate between adjacent endothelial cells. However, well-established endothelial cell contacts with tight junctions are CD34 negative. CD34 may function as an adhesion molecule on both endothelial cells and hematopoietic progenitors.

scholarly journals Expression of the CD34 gene in vascular endothelial cells

Blood ◽  
1990 ◽  
Vol 75 (12) ◽  
pp. 2417-2426 ◽  
Author(s):  
L Fina ◽  
HV Molgaard ◽  
D Robertson ◽  
NJ Bradley ◽  
P Monaghan ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelium ◽  
Umbilical Artery ◽  
Vascular Endothelial Cells ◽  
Umbilical Vein ◽  
Vascular Endothelial ◽  
Umbilical Vessel ◽  
Cd34 Antigen ◽  
Large Vessels ◽  
Hemopoietic Progenitor

All seven of a set of CD34 monoclonal antibodies that recognize epitopes on an approximately 110 Kd glycoprotein on human hemopoietic progenitor cells also bind to vascular endothelium. Capillaries of most tissues are CD34 positive, as are umbilical artery and, to a lesser extent, vein, but the endothelium of most large vessels and the endothelium of placental sinuses are not. Angioblastoma cells and parafollicular mesenchymal cells in fetal skin are also CD34 positive, as are some stromal elements. An approximately 110 Kd protein can be identified by Western blot analysis with CD34 antibodies in detergent extracts of freshly isolated umbilical vessel endothelial cells, and CD34 mRNA is present in cultured umbilical vein cells as well as other tissues rich in vascular endothelium (breast, placenta). These data indicate that the binding of CD34 antibodies to vascular endothelium is to the CD34 gene product, and not to crossreactive epitopes. Despite the presence of CD34 mRNA in cultured, proliferating endothelial cells, the latter do not bind CD34 antibodies. In addition, CD34 antigen cannot be upregulated by growth factors. We conclude that under these conditions, CD34 protein is downregulated or processed into another form that is unreactive with CD34 antibodies. Electron microscopy of umbilical artery, breast, and kidney capillary vessels reveals that in all three sites, CD34 molecules are concentrated on membrane processes, many of which interdigitate between adjacent endothelial cells. However, well-established endothelial cell contacts with tight junctions are CD34 negative. CD34 may function as an adhesion molecule on both endothelial cells and hematopoietic progenitors.

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