Effect of tumor promoting stimuli on gap junction permeability and connexin 43 expression in ARL 18 rat liver cell line

1993 ◽  
Vol 67 (8) ◽  
pp. 565-572 ◽  
Author(s):  
Irina V. Budunova ◽  
Gary M. Williams ◽  
David C. Spray
Keyword(s):  
Gap Junction ◽  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Connexin 43 ◽  
Liver Cell Line

scholarly journals Pancreatic Transdifferentiation and Glucose-Regulated Production of Human Insulin in the H4IIE Rat Liver Cell Line

2016 ◽  
Vol 17 (4) ◽  
pp. 534 ◽  
Author(s):  
Binhai Ren ◽  
Chang Tao ◽  
Margaret Swan ◽  
Nichole Joachim ◽  
Rosetta Martiniello-Wilks ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Insulin ◽  
Rat Liver ◽  
Liver Cell ◽  
Liver Cell Line

Multiplication stimulating activity (MSA) from the BRL 3A rat liver cell line: Relation to human somatomedins and insulin

1981 ◽  
Vol 15 (3) ◽  
pp. 253-286 ◽  
Author(s):  
Matthew M. Rechler ◽  
S. Peter Nissley ◽  
George L. King ◽  
Alan C. Moses ◽  
Ellen E. Van Obberghen-Schilling ◽  
...  
Keyword(s):  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Liver Cell Line

scholarly journals Establishment of a fetal rat liver cell line that retains differentiated liver functions.

1980 ◽  
Vol 77 (5) ◽  
pp. 2731-2734 ◽  
Author(s):  
S. E. Schlegel-Haueter ◽  
W. Schlegel ◽  
J. Y. Chou
Keyword(s):  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Liver Cell Line ◽  
Fetal Rat ◽  
Liver Functions ◽  
Fetal Rat Liver

Distinctive gap junction channel types connect WB cells, a clonal cell line derived from rat liver

1991 ◽  
Vol 260 (3) ◽  
pp. C513-C527 ◽  
Author(s):  
D. C. Spray ◽  
M. Chanson ◽  
A. P. Moreno ◽  
R. Dermietzel ◽  
P. Meda
Keyword(s):  
Gap Junction ◽  
Cell Line ◽  
Rat Liver ◽  
Connexin 43 ◽  
Single Channel ◽  
Freeze Fracture ◽  
Particle Sizes ◽  
Frequency Distributions ◽  
Gap Junction Channel ◽  
Junctional Conductance

Gap junctions, dye coupling, and junctional conductance were studied in a cell line (WB) that is derived from rat liver and displays a phenotype similar to “oval” cells. In freeze-fracture replicas, two distinctive particle sizes were detected in gap junctional plaques. Immunocytochemical studies indicated punctate staining at membrane appositions using antibodies to connexin 43 and to a brain gap junction-associated antigen (34 kDa). No staining was observed using antibodies prepared against rat liver gap junction proteins (connexins 32 and 26). Pairs of WB cells were electrically and dye coupled. Junctional conductance (gj) between cell pairs averaged approximately 10 nS; occasionally, gj was low enough that unitary junctional conductances (gamma j) could be detected. Using a CsCl-containing electrode solution, distinctive gamma j values were recorded: approximately 20-30 pS, approximately 80-90 pS, and the sum of the other sizes. The largest gamma j events were apparently due to random coincident openings or closures of the smaller channels. Several treatments reduced gj. Frequency distributions of gamma j were unaltered by 2 mM halothane or 3.5 heptanol, but the sizes of intermediate and largest events were reduced slightly by 100 nM phorbol ester, and the relative frequency of the largest events was increased by 10 microM glutaraldehyde. We conclude that the distinctive gamma j values represent openings and closures of two distinct types of gap junction channels rather than substates of a single channel type; these unitary conductances may correspond to the dual immunoreactivity and to the two particle sizes seen in freeze fracture.

Thyroid hormone induction of Na(+)-K(+)-ATPase and its mRNAs in a rat liver cell line

1990 ◽  
Vol 258 (3) ◽  
pp. C544-C551 ◽  
Author(s):  
G. G. Gick ◽  
F. Ismail-Beigi
Keyword(s):  
Atpase Activity ◽  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Northern Blot Analysis ◽  
Fold Increase ◽  
Beta Subunits ◽  
Inhibit Protein Synthesis ◽  
Liver Cell Line ◽  
Stimulation Of

The expression of mRNAs encoding the alpha- and beta-subunits of Na(+)-K(+)-ATPase (Na(+)-K+ pump) was examined in a rat liver cell line, Clone 9, in various thyroidal states. Northern blot analysis of total RNA isolated from cells incubated in hypothyroid serum-containing medium revealed the expression of mRNAs encoding Na(+)-K(+)-ATPase alpha 1-(mRNA alpha 1) and beta- (mRNA beta) subunits; mRNAs encoding the alpha 2- and alpha 3-subunits were undetectable. There was a discrepancy in the abundance of mRNA alpha 1 relative to mRNA beta such that mRNA alpha 1 exceeded the sum of the multiple mRNA beta bands by approximately 35-fold. 3,3',5-Triiodothyronine (T3) produced a coordinate augmentation of mRNA alpha 1 and mRNA beta contents that was demonstrable within 2 h and preceded the stimulation of Na(+)-K(+)-ATPase activity. After incubation of cells with T3 for 48 h, Na(+)-K(+)-ATPase activity was stimulated by 1.32-fold, whereas mRNA alpha 1 and mRNA beta abundances were increased 1.46- and 2.87-fold, respectively. Treatment of cells for 6 h with 10 micrograms/ml cycloheximide, a concentration sufficient to inhibit protein synthesis by 95%, elicited a 3.5- and 5.1-fold increase in mRNA alpha 1 and mRNA beta content, respectively. Cycloheximide abrogated the stimulatory effect of T3 on mRNA beta abundance, whereas the T3-induced increase in mRNA alpha 1 content was not prevented.(ABSTRACT TRUNCATED AT 250 WORDS)

Stimulation of Active Na+and K+Transport by Thyroid Hormone in a Rat Liver Cell Line: Role of Enhanced Na+Entry*

Endocrinology ◽  
1986 ◽  
Vol 119 (6) ◽  
pp. 2527-2536 ◽  
Author(s):  
FARAMARZ ISMAIL-BEIGI ◽  
RICHARD S. HABER ◽  
JOHN N. LOEB
Keyword(s):  
Thyroid Hormone ◽  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Liver Cell Line ◽  
K Transport ◽  
Stimulation Of

Inhibitory Effects of Capsaicinoids on Fatty Acid Desaturation in a Rat Liver Cell Line

2001 ◽  
Vol 65 (8) ◽  
pp. 1859-1863 ◽  
Author(s):  
Nobuhiro NAKANO ◽  
Norifumi SHIRASAKA ◽  
Kazuki MASUOKA ◽  
Tetsuo MURAKAMI ◽  
Tatsuo WATANABE ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Fatty Acid Desaturation ◽  
Inhibitory Effects ◽  
Liver Cell Line

scholarly journals A new member of therasgene superfamily identified in a rat liver cell line

1988 ◽  
Vol 16 (21) ◽  
pp. 9979-9993 ◽  
Author(s):  
Cecilia Bucci ◽  
Rodolfo Frunzio ◽  
Lorenzo Chiariotti ◽  
Alexandra L. Brown ◽  
Matthew M. Rechler ◽  
...  
Keyword(s):  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Liver Cell Line
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