Tylose formation in elms after inoculation with an aggressive or a non-aggressive strain of Ophiostoma ulmi or with a nonpathogen to elms

1977 ◽  
Vol 83 (6) ◽  
pp. 241-243 ◽  
Author(s):  
D. M. Elgersma ◽  
H. J. Miller
Keyword(s):  
Ophiostoma Ulmi ◽  
Tylose Formation

Accumulation of mansonones E and F in seedlings of Ulmus americana in response to inoculation with Ophiostoma ulmi

Trees ◽  
1990 ◽  
Vol 4 (4) ◽  
Author(s):  
LucC. Duchesne ◽  
R.S. Jeng ◽  
M. Hubbes ◽  
M.B. Sticklen
Keyword(s):  
Ulmus Americana ◽  
Ophiostoma Ulmi

Ophiostoma ulmi metabolites and elm cell membrane permeability. Possible use in early tests of resistance

1988 ◽  
Vol 18 (2) ◽  
pp. 77-84 ◽  
Author(s):  
B. Mezzetti ◽  
L. Mittempergher ◽  
P. Rosati
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Cell Membrane Permeability ◽  
Ophiostoma Ulmi

Analytical electrofocusing and two-dimensional electrophoresis of proteins extracted from the mycelia of aggressive and nonaggressive strains of Ophiostoma ulmi

1986 ◽  
Vol 64 (9) ◽  
pp. 2073-2081 ◽  
Author(s):  
Robert S. Jeng
Keyword(s):  
Dutch Elm Disease ◽  
Two Dimensional ◽  
Polyacrylamide Gels ◽  
Ophiostoma Ulmi ◽  
Two Dimensional Electrophoresis ◽  
Coomassie Blue ◽  
Isoelectric Points ◽  
Additional Protein ◽  
Dimensional Electrophoresis ◽  
Electrophoresis Of Proteins

Soluble mycelial proteins from Ophiostoma ulmi (Buism.) Nannf., the causal agent of Dutch elm disease, were separated by analytical electrofocusing and two-dimensional electrophoresis in polyacrylamide gels. Results showed the aggressive and nonaggressive strains of this pathogen each had about 60 Coomassie blue stained bands having isoelectric points from 3 to 7. Both strains of this fungus had their own characteristic electrofocusing patterns. Nonaggressive isolate S116, for example, lacked two protein bands, one near the anode and one near the cathode, but it had five additional protein bands distributed from pH 4 to 6. Two-dimensional electrophoresis of total soluble proteins depicted that there were 36 proteins found to be specific for the nonaggressive isolate S116 and 12 proteins for the aggressive isolate RR2.

Analysis of a high frequency transformation system for Ophiostoma ulmi, the causal agent of Dutch elm disease

1991 ◽  
Vol 225 (1) ◽  
pp. 168-176 ◽  
Author(s):  
J. C. Royer ◽  
K. Dewar ◽  
M. Hubbes ◽  
P. A. Horgen
Keyword(s):  
High Frequency ◽  
Causal Agent ◽  
Dutch Elm Disease ◽  
Transformation System ◽  
Ophiostoma Ulmi ◽  
Frequency Transformation ◽  
High Frequency Transformation

scholarly journals The occurrence of endophytic fungus Phomopsis oblonga on elms in the area of southern Bohemia

2012 ◽  
Vol 52 (No. 11) ◽  
pp. 531-535 ◽  
Author(s):  
M. Dvořák ◽  
D. Palovčíková ◽  
L. Jankovský
Keyword(s):  
Endophytic Fungus ◽  
Health Condition ◽  
Dutch Elm Disease ◽  
Mixed Forests ◽  
Ophiostoma Ulmi

The health condition of the population of elms in the region of southern Bohemiawas studied from the viewpoint of their decline, the occurrence of Dutch Elm Disease (DED) and the presence of other diseases. Of the total number of 105 elms in total 33 of them were without any symptoms of the disease or other damage. Elms regenerated quite spontaneously in the neighbourhood of mother trees and their increasing population in mixed forests is hopeful. According to macroscopic symptoms, DED was identified in 10 trees but the presence of pathogens Ophiostoma ulmi and Ophiostoma novo-ulmi was not identified in isolations. A possible reason of this observation is overgrowing the colonies by the Phomopsis oblonga mycelium. This fungus was identified in most isolations. Thus, its role requires further research.

Isolation and ultrastructural localization of a soluble protein from Ophiostoma ulmi

1990 ◽  
Vol 68 (11) ◽  
pp. 2517-2524 ◽  
Author(s):  
R. S. Jeng ◽  
A. M. Svircev
Keyword(s):  
Polyclonal Antibodies ◽  
Polyacrylamide Gel Electrophoresis ◽  
Protein A ◽  
Leading Edge ◽  
Ultrastructural Localization ◽  
Immunogold Labelling ◽  
Two Dimensional ◽  
Thin Sections ◽  
Ophiostoma Ulmi ◽  
Gold Label

Two-dimensional polyacrylamide gel electrophoresis was used to identify and isolate a soluble polypeptide, the QP1 protein, which is characteristic of the vegetative hyphae of nonaggressive isolate Q412 of Ophiostoma ulmi. Individual QP1 spots were excised from 16 two-dimensional gels. Polypeptides were eluted from the gel spots by electroelution and lyophilized. The protein was injected into rabbits for the production of polyclonal antibodies. Antiserum specificity was tested by transferring polypeptides from a two-dimensional gel onto nitrocellulose and treating with QP1 serum. The resulting immunoblot contained a single spot that corresponded in shape and location to that of the QP1 polypeptide. Thin sections of fungal mycelia, from nonaggressive isolate Q412 and the aggressive isolate VA of O. ulmi, were treated with QP1 antibodies and protein A – gold. The gold label was localized in thin sections over conidial and hyphal cell walls of the nonaggressive isolate. The aggressive isolate was nonreactive. Mycelia from nonaggressive isolates Q412 and Q311 and aggressive isolates VA and CESS16K of O. ulmi were grown on solid medium, treated with QP1 antibodies, labelled with protein A – gold, and prepared for scanning electron microscopy. The gold-labelled QP1 polypeptide was detected on the leading edge of a small number of hyphae from nonaggressive isolates Q412 and Q311. Key words: immunogold labelling, Ophiostoma ulmi, soluble proteins.

scholarly journals Contribution to identify the causal gents of Dutch elm disease in the Czech Republic

2008 ◽  
Vol 43 (No. 4) ◽  
pp. 142-145 ◽  
Author(s):  
M. Dvořák ◽  
M. Tomšovský ◽  
L. Jankovský ◽  
D. Novotný
Keyword(s):  
Czech Republic ◽  
North America ◽  
Molecular Biology ◽  
The Other ◽  
Dutch Elm Disease ◽  
The Czech Republic ◽  
Ophiostoma Ulmi ◽  
Other Hand ◽  
Subspecies Level ◽  
First Time

This study provides new data on Dutch elm disease in the Czech Republic. <I>Ophiostoma novo-ulmi</I> is reported for the first time in the area of the Czech Republic, as well as both subspecies ssp. <I>novo-ulmi</I> (indigenous in the area of the Ukraine and Moldavia), and ssp. <I>Americana</I> indigenous in North America. The majority of the recorded strains belonged to <I>O. n.-u.</I> ssp. <I>novo-ulmi</I>, while <I>O. n.-u.</I> ssp. <I>Americana</I> and hybrids of these two subspecies were found less frequently. On the other hand, <I>Ophiostoma ulmi</I> was not found at all in the investigated samples. Identification on the subspecies level was performed by methods of molecular biology, i.e. PCR and RFLP of gene regions<I> cu</I> and <I>col1</I>.

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