A rapid magnetizable solid phase radioimmunoassay kit for human placental lactogen

Abstract We report a solid-phase radioimmunoassay for human placental lactogen, in which antibody-coated polystyrene tubes and polyurethane sponges are used. Incubation time and temperature, ionic strength of buffer, and nonspecific adsorption have been evaluated for optimum conditions required for routine clinical assays. The validity of the assay technique has been ascertained by its specificity, reproducibility, and recovery. The sensitivity of this method is 0.4 ng of placental lactogen per assay tube.

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Observations on the use of solid-phase-coupled antibodies in the radioimmunoassay of human placental lactogen

Biochemical Journal ◽

10.1042/bj1370469 ◽

1974 ◽

Vol 137 (3) ◽

pp. 469-476 ◽

Cited By ~ 7

Author(s):

Jacqueline Gardner ◽

Gordon Bailey ◽

Timothy Chard

Keyword(s):

Solid Phase ◽

Coupling Reaction ◽

High Sensitivity ◽

Comparative Assessment ◽

Placental Lactogen ◽

Affinity Constant ◽

Considerable Proportion ◽

Lower Sensitivity ◽

Human Placental Lactogen ◽

Free Antigen

A detailed comparative assessment was made of the use of solid-phase-coupled antibodies in radioimmunoassay, by using an assay for human placental lactogen as a model system. The major advantages of the solid-phase technique are: (1) in common with the use of a second antibody, it is universally applicable; (2) separation can be carried out rapidly; (3) in contrast with some other techniques, the separation of antibody-bound and free antigen is virtually complete. The disadvantages when compared with other procedures are: (1) a considerable proportion of the antibody may be lost during the initial coupling reaction; (2) the tubes must be continuously mixed during incubation, and much effort is expended in removing and replacing the caps; (3) there is a decrease in the apparent affinity constant of the antibody after coupling, which is reflected in a lower sensitivity of the assay system. It is concluded that solid-phase antibodies are of greatest value in those systems in which the supply of antiserum is abundant, and in which the achievement of high sensitivity is not a requirement.

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Radioimmunoassay of Fibrinogen and Its Proteolysis Products

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651243 ◽

1968 ◽

Vol 20 (01/02) ◽

pp. 001-006 ◽

Cited By ~ 5

Author(s):

K. J Catt ◽

J Hirsh ◽

D. J Castelan ◽

H. D Niall ◽

G. W Tregear

Keyword(s):

Solid Phase ◽

Solid Phase Radioimmunoassay ◽

Radioimmunoassay Method

SummaryThe solid-phase radioimmunoassay method has been applied to the measurement of fibrinogen. The method is extremely sensitive, being able to detect fibrinogen concentrations as low as 10 ng/ml. The immunoreactivity of fibrinogen proteolysis products differs from that of native fibrinogen, early proteolysis products showing enhanced immunoreactivity which decreases progressively with further digestion.

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