Awareness of ORAC assay among dental students

The Oxygen Radical Absorption Capacity (ORAC) assay estimates the suppression of radical-mediated oxidation by molecules of concern. This calculates the quality as equivalent and provides both repression duration and the degree of oxidation suppression. This same test could be used to calculate the antioxidant capacity of nutrition. The survey was performed for assessing awareness of ORAC assay amongst dental students. This was a questionnaire implemented cross-sectional type of survey comprising 100 dental college students in Chennai. A self-designed questionnaire with 10 queries eliciting the knowledge and awareness about applications of the ORAC assay technique was distributed among dental college students. Questionnaires were circulated through an online website survey planet. The questions explored the awareness of ORAC assay technique, diagnostic indications ofORAC assay technique, limitations ofORAC assay technique and mechanism of ORAC assay technique. After the responses were received from 100 participants, data were collected and . 67% of the respondents were aware of the ORAC assay technique .52% were aware of diagnostic indications of the ORAC assay technique. 45%% were aware of the limitations of the ORAC assay technique. 35% were aware of the mechanism of the ORAC assay technique. The awareness about the ORAC assay technique and its applications were less among dental students. Increased awareness and educational programs should be initiated to spread knowledge about the ORAC assay technique among all students and clinicians.

Polymorphism in GSTM1 and GSTT1 genes influence DNA damage in personnel occupationally exposed to volatile anaesthetics (VA), from Peshawar, Pakistan

ObjectivesThe objective of this study was to assess the influence of antioxidant gene GSTM1 and GSTT1 on DNA damage in personnel occupationally exposed to volatile anaesthetics (VA).MethodsThe study groups were composed of 50 exposed subjects (anaesthesia workers) and 49 controls. Blood samples were collected from both subjects. DNA damage was analysed through the comet assay technique. Biomarker genes GSTM1 and GSTT1 were inspected through PCR technique for polymorphism.ResultsThe comet assay technique showed that the Total Comet Score (TCS) in exposed subjects was significantly higher (p=0.0001) than the control. Age and smoking had significant effects on TCS in the study groups (p<0.05). Duration of occupational exposure had significant positive correlation (r=0.755, p<0.001) with DNA damage. The null polymorphism in GSTM1 and GSTT1 gene showed a significant effect (p<0.001 and p<0.000) on the DNA damage.ConclusionsThe polymorphism in GSTM1 and GSTT1 gene significantly damage DNA in personnel occupationally exposed to VA.